7 research outputs found
Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe
The Replicative Consequences of Papillomavirus E2 Protein Binding to the Origin Replication Factor ORC2
Genome-wide analysis of replication timing by next-generation sequencing with E/L Repli-seq
A journey through the microscopic ages of DNA replication
Scientific discoveries and technological advancements are inseparable but not always take place in a coherent chronological manner. In the next, we will provide a seemingly unconnected and serendipitous series of scientific facts that, in the whole, converged to unveil DNA and its duplication. We will not cover here the many and fundamental contributions from microbial genetics and in vitro biochemistry. Rather, in this journey, we will emphasize the interplay between microscopy development culminating on super resolution fluorescence microscopy (i.e., nanoscopy) and digital image analysis and its impact on our understanding of DNA duplication. We will interlace the journey with landmark concepts and experiments that have brought the cellular DNA replication field to its present state