Five Decades of Research on Mitochondrial NADH-quinone Oxidoreductase (complex I)

NADH-quinone oxidoreductase (complex I) is the largest and most complicated enzyme complex of the mitochondrial respiratory chain. It is the entry site into the respiratory chain for most of the reducing equivalents generated during metabolism, coupling electron transfer from NADH to quinone to proton translocation, which in turn drives ATP synthesis. Dysfunction of complex I is associated with neurodegenerative diseases such as Parkinson’s and Alzheimer’s, and it is proposed to be involved in aging. Complex I has one non-covalently bound FMN, eight to 10 iron-sulfur clusters, and protein-associated quinone molecules as electron transport components. Electron paramagnetic resonance (EPR) has previously been the most informative technique, especially in membrane in situ analysis. The structure of complex 1 has now been resolved from a number of species, but the mechanisms by which electron transfer is coupled to transmembrane proton pumping remains unresolved. Ubiquinone-10, the terminal electron acceptor of complex I, is detectable by EPR in its one electron reduced, semiquinone (SQ) state. In the aerobic steady state of respiration the semi-ubiquinone anion has been observed and studied in detail. Two distinct protein-associated fast and slow relaxing, SQ signals have been resolved which were designated SQNf and SQNs. This review covers a five decade personal journey through the field leading to a focus on the unresolved questions of the role of the SQ radicals and their possible part in proton pumping

Studying Diquark Structure of Heavy Baryons in Relativistic Heavy Ion Collisions

We propose the enhancement of $\Lambda_c$ yield in heavy ion collisions at RHIC and LHC as a novel signal for the existence of diquarks in the strongly coupled quark-gluon plasma produced in these collisions as well as in the $\Lambda_c$. Assuming that stable bound diquarks can exist in the quark-gluon plasma, we argue that the yield of $\Lambda_c$ would be increased by two-body collisions between $ud$ diquarks and $c$ quarks, in addition to normal three-body collisions among $u$, $d$ and $c$ quarks. A quantitative study of this effect based on the coalescence model shows that including the contribution of diquarks to $\Lambda_c$ production indeed leads to a substantial enhancement of the $\Lambda_c/D$ ratio in heavy ion collisions.Comment: Prepared for Chiral Symmetry in Hadron and Nuclear Physics (Chiral07), Nov. 13-16, 2007, Osaka, Japa

Tables of Hyperonic Matter Equation of State for Core-Collapse Supernovae

We present sets of equation of state (EOS) of nuclear matter including hyperons using an SU_f(3) extended relativistic mean field (RMF) model with a wide coverage of density, temperature, and charge fraction for numerical simulations of core collapse supernovae. Coupling constants of Sigma and Xi hyperons with the sigma meson are determined to fit the hyperon potential depths in nuclear matter, U_Sigma(rho_0) ~ +30 MeV and U_Xi(rho_0) ~ -15 MeV, which are suggested from recent analyses of hyperon production reactions. At low densities, the EOS of uniform matter is connected with the EOS by Shen et al., in which formation of finite nuclei is included in the Thomas-Fermi approximation. In the present EOS, the maximum mass of neutron stars decreases from 2.17 M_sun (Ne mu) to 1.63 M_sun (NYe mu) when hyperons are included. In a spherical, adiabatic collapse of a 15$M_\odot$ star by the hydrodynamics without neutrino transfer, hyperon effects are found to be small, since the temperature and density do not reach the region of hyperon mixture, where the hyperon fraction is above 1 % (T > 40 MeV or rho_B > 0.4 fm^{-3}).Comment: 23 pages, 6 figures (Fig.3 and related comments on pion potential are corrected in v3.

SuprimeCam Observation of Sporadic Meteors during Perseids 2004

We report the serendipitous findings of 13 faint meteors and 44 artificial space objects by Subaru SuprimeCam imaging observations during 11-16 August 2004. The meteors, at about 100km altitude, and artificial satellites/debris in orbit, at 500km altitude or higher, were clearly discriminated by their apparent defocused image sizes. CCD photometry of the 13 meteors, including 1 Perseid, 1 Aquarid, and 11 sporadic meteors, was performed. We defined a peak video-rate magnitude by comparing the integrated photon counts from the brightest portion of the track traversed within 33ms to those from a 0-mag star during the same time duration. This definition gives magnitudes in the range 4.0< V_{vr} <6.4 and 4.1< I_{vr}<5.9 for these 13 meteors. The corresponding magnitude for virtual naked-eye observers could be somewhat fainter especially for the V-band observation, in which the [OI] 5577 line lasting about 1 sec as an afterglow could contribute to the integrated flux of the present 5-10 min CCD exposures. Although the spatial resolution is insufficient to resolve the source size of anything smaller than about 1 m, we developed a new estimate of the collisionally excited column diameter of these meteors. A diameter as small as a few mm was derived from their collisionally excited photon rates, meteor speed, and the volume density of the oxygen atoms at the 100km altitude. The actual column diameter of the radiating zone, however, could be as large as few 100m because the excited atoms travel that distance before they emit forbidden lines in 0.7 sec of its average lifetime. Among the 44 artificial space objects, we confirmed that 17 were cataloged satellites/space debris.Comment: 14 pages, 13 figures, 5 tables, submitted to PAS

The Dichotomy of the Halo of the Milky Way

We summarize evidence that the halo of the Milky Way comprises two different, and broadly overlapping, stellar components. The two structures exhibit different chemical compositions, spatial distributions, and kinematics. These results were obtained through an analysis of more than 20,000 calibration stars from the Sloan Digital Sky Survey (SDSS). The duality of the stellar halo directly impacts galaxy formation models, for the Milky Way and other large spirals.Comment: 6 pages, 4 figures, to appear in Proceedings of the OMEG07 Conference, held in December 200

Pathophysiology of acute experimental pancreatitis: Lessons from genetically engineered animal models and new molecular approaches

The incidence of acute pancreatitis is growing and worldwide population-based studies report a doubling or tripling since the 1970s. 25% of acute pancreatitis are severe and associated with histological changes of necrotizing pancreatitis. There is still no specific medical treatment for acute pancreatitis. The average mortality resides around 10%. In order to develop new specific medical treatment strategies for acute pancreatitis, a better understanding of the pathophysiology during the onset of acute pancreatitis is necessary. Since it is difficult to study the early acinar events in human pancreatitis, several animal models of acute pancreatitis have been developed. By this, it is hoped that clues into human pathophysiology become possible. In the last decade, while employing molecular biology techniques, a major progress has been made. The genome of the mouse was recently sequenced. Various strategies are possible to prove a causal effect of a single gene or protein, using either gain-of-function (i.e., overexpression of the protein of interest) or loss-of-function studies (i.e., genetic deletion of the gene of interest). The availability of transgenic mouse models and gene deletion studies has clearly increased our knowledge about the pathophysiology of acute pancreatitis and enables us to study and confirm in vitro findings in animal models. In addition, transgenic models with specific genetic deletion or overexpression of genes help in understanding the role of one specific protein in a cascade of inflammatory processes such as pancreatitis where different proteins interact and co-react. This review summarizes the recent progress in this field. Copyright (c) 2005 S. Karger AG, Basel

Universality in metallic nanocohesion: a quantum chaos approach

Convergent semiclassical trace formulae for the density of states and cohesive force of a narrow constriction in an electron gas, whose classical motion is either chaotic or integrable, are derived. It is shown that mode quantization in a metallic point contact or nanowire leads to universal oscillations in its cohesive force: the amplitude of the oscillations depends only on a dimensionless quantum parameter describing the crossover from chaotic to integrable motion, and is of order 1 nano-Newton, in agreement with recent experiments. Interestingly, quantum tunneling is shown to be described quantitatively in terms of the instability of the classical periodic orbits.Comment: corrects spelling of one author name on abstract page (paper is unchanged