Understanding the performance of water supply systems during mild to extreme droughts

This project assessed the performance of different types of public water supply systems in England and Wales in a range of droughts, including those that are more severe than the worst droughts in the historical record

Hybridization of seedless grapes

Complete seedlessness was not achieved in any seedless x seedless families produced. This clearly shows the seedless trait is not controlled by a single recessive gene.The percentage of seedlessness obtained was not the same for all families. This indicates the importance of parental combinations and the need for progeny testing to increase the efficiency of producing seedless offspring.All seedless genotypes used except Thompson Seedless have a seeded female parent, indicating heterogenity for seeds/aborted seeds. It will be interesting now to use seedlings from the seedless x seedless families to see if 100 % seedless offspring can be obtained.Three seedless x seedless families compared to their complementary families from seeded x seedless were significantly different for percent seedless. In ovulo embryo rescue of seedless x seedless hybrids is also advantageous as it allows direct hybridization without seeded genotypes. This eliminates the introduction of genes from seeded genotypes and one generation - a savings of 5 years. The ability to achieve complimentary crosses between seedless genotypes directly can be achieved

Long-lived heteronuclear spin-singlet states

We report observation of long-lived spin-singlet states in a 13C-1H spin pair at zero magnetic field. In 13C-labeled formic acid, we observe spin-singlet lifetimes as long as 37 seconds, about a factor of three longer than the T1 lifetime of dipole polarization in the triplet state. We also observe that the lifetime of the singlet-triplet coherence, T2, is longer than T1. Moreover, we demonstrate that this singlet states formed by spins of a heteronucleus and a 1H nucleus, can exhibit longer lifetimes than the respective triplet states in systems consisting of more than two nuclear spins. Although long-lived homonuclear spin-singlet states have been extensively studied, this is the first experimental observation of analogous spin-singlets consisting of a heteronucleus and a proton.Comment: 5 pages, 4 figure

Event Order in the Biathlon Does Not Have an Effect on Metabolic Response

The purpose of this study was to examine the effects of event order on a cycling(C)/running(R) or R/C biathlon. Eight experienced male biathlete/triathletes with a mean age of 24.9 + 4.6 yr formed the sample of the study. Results show no significant interaction effects on oxygen consumption peak, oxygen consumption during steady-state, ventilation, and heart rate when C/R or R/C are performed at 70% oxygen consumption peak for subsequent R and C respectively. These results seem to indicate that the biathlete/triathlete is efficient in both C and R to the extent that event order does not significantly interact with metabolic response in submaximal cycling and running

Engaging stakeholders in research to address water-energy-food (WEF) nexus challenges

The water–energy–food (WEF) nexus has become a popular, and potentially powerful, frame through which to analyse interactions and interdependencies between these three systems. Though the case for transdisciplinary research in this space has been made, the extent of stakeholder engagement in research remains limited with stakeholders most commonly incorporated in research as end-users. Yet, stakeholders interact with nexus issues in a variety of ways, consequently there is much that collaboration might offer to develop nexus research and enhance its application. This paper outlines four aspects of nexus research and considers the value and potential challenges for transdisciplinary research in each. We focus on assessing and visualising nexus systems; understanding governance and capacity building; the importance of scale; and the implications of future change. The paper then proceeds to describe a novel mixed-method study that deeply integrates stakeholder knowledge with insights from multiple disciplines. We argue that mixed-method research designs—in this case orientated around a number of cases studies—are best suited to understanding and addressing real-world nexus challenges, with their inevitable complex, non-linear system characteristics. Moreover, integrating multiple forms of knowledge in the manner described in this paper enables research to assess the potential for, and processes of, scaling-up innovations in the nexus space, to contribute insights to policy and decision making

A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones

The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ hybridization (FISH) with whole chromosome painting probes derived from chromosome-specific DNA libraries and Alu-polymerase chain reaction (PCR) products of various region-specific yeast artificial chromosome (YAC) clones. To demonstrate the usefulness of this strategy for the characterization of chromosome rearrangements unidentifiable by banding techniques, an 8p+ marker chromosome with two extra bands present in the karyotype of a child with multiple anomalies, malformations, and severe mental retardation was investigated. A series of seven-color FISH experiments with sets of fluorochrome-labeled DNA library probes from flow-sorted chromosomes demonstrated that the additional segment on 8p+ was derived from chromosome 6. For a more detailed characterization of the marker chromosome, three-color FISH experiments with library probes specific to chromosomes 6 and 8 were performed in combination with newly established telomeric and subtelomeric YAC clones from 6q25, 6p23, and 8p23. These experiments demonstrated a trisomy 6pter6p22 and a monosomy 8pter8p23 in the patient. The present limitations for a broad application of this strategy and its possible improvements are discusse