INTRODUCTION : THE EU-NETWORK CRAYNET- IMPACTS ON FUNDAMENTAL QUESTIONS

The European network CRAYNET “European crayfish as keystone species-linking science, management and economics with sustainable environmental quality” emphasises knowledge-based management strategies. The CRAYNET meetings concentrate on certain topics: monitoring in conservation and management of natives; interaction between natives and aliens; control of aliens; habitat restoration; reintroduction and restocking; legislation; education. The Irish meeting (Kilkenny, 2003) “The endangered native crayfish Austropotamobius pallipes: bioindicator and heritage species” initiated debates about (1) what is a bioindicator, a flagship species or a heritage species?; (2) the problem of species complexes and the need to clarify and resolve taxonomy before management; and (3) what is meant by biodiversity in the context of European crayfish? The Norwegian meeting (Halden, 2003) “European native, crayfish with a special focus on Astacus astacus: linking socioeconomics and conservation ” involved roundtables about (1) threats to indigenous population of crayfish at a landscape level; (2) exploitation, conservation, legislation; (3) reintroduction of indigenous crayfish, habitat restoration and monitoring; and (4) the urgent need for joint research about pathology. The present meeting (Innsbruck, 2004) “European native crayfish in relation to land-use and habitat deterioration with a special focus on Austropotamobius torrentim” aims to identify the species protection programs, to examine if legislation is working well in protecting vulnerable species, the state of advances about conservation genetics and how to make progress in education of the public. A permanent connection between the 12 core members provides opportunities to check the needed lines of research, to prepare recommendations, and to gather information for an atlas on the distribution of crayfish species in Europe as well as to prepare leaflets for the public

Statins are underused in recent-onset Parkinson's disease with increased vascular risk : findings from the UK Tracking Parkinson's and Oxford Parkinson's Disease Centre (OPDC) discovery cohorts

Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.Peer reviewedPublisher PD

Bacterial diversity on stainless steel surfaces of egg processing companies and potential of selected isolates to spoil liquid whole egg products

AimsTo assess the bacterial diversity in the French egg processing industry and to explore the adhesion and spoilage potential of selected bacteria.Methods and ResultsSterile stainless steel chips were suspended for 2 months inside the pipelines of seven egg processing companies, before and after the pasteurizer, at warm and cold seasons. After exposure, the bacterial diversity was assessed by 16S rDNA sequencing. The 231 collected isolates were mainly facultative anaerobic Gram positive bacteria, such as Streptococcus, Staphylococcus, Bacillus and Kocuria. Sixty‐five representative isolates were further characterized in vitro regarding the potential for adhesion and egg product spoilage. A high diversity was observed from one genus to another. Kocuria and Rothia isolates showed significantly higher adhesion than the isolates of the other genera. Only the isolates belonging to the genera Bacillus and Lysinibacillus, associated with high enzymatic activities on a solid egg‐based medium, were able to induce spoilage of liquid whole egg.ConclusionsBacteria collected on stainless steel surfaces placed in egg processing industries could be associated to liquid egg product spoilage.Significance and Impact of the StudyThis study provides new insights on the bacterial contamination in egg processing companies and represents a first step for the effective control of undesirable bacteria in liquid egg products

Conserving indigenous crayfish: stock assessment and habitat requirements in the threatened Austropotamobius italicus.

International audienceAs part of the Austropotamobius pallipes species complex, the crayfish Austropotamobius italicus is a species of community interest whose preservation requires the designation of Special Areas of Conservation (SACs) (Annex II, EU Habitats Directive). This study aimed at (1) assessing the conservation status of this threatened indigenous species by stock assessment in central Italy and (2) identifying some aspects of its elective habitat

Tolerance of three European native species of crayfish to hypoxia.

Species that can act as indicators of ecosystem health offer a valuable tool in the management of natural resources. Crayfish have been suggested as bioindicators of water quality in Europe and at least one species (Austropotamobius pallipes) has been studied to determine its tolerance to pollution and its potential as a bioindicator. The genus Austropotamobius includes three crayfish species native to western Europe: A. pallipes, A. italicus and A. torrentium. It was hypothesised that because of their geographical and habitat distribution, the three Austropotamobius species might vary in their value as a bioindicator of water quality. Crayfish of species A. pallipes and A. italicus were subjected to three different treatments: hypoxia (treatment 3, approx 3 mg 1(-1) O-2), light hypoxia (treatment 2, approx 5.5 mg 1(-1) O-2) and normoxia (treatment 1, control, approx 8.5 mg 1(-1) O-2). A. torrentium crayfish were only subjected to treatment 1 (control) and 3. Variations in haemolymph sodium, calcium and chloride were used as a biomarker and concentrations were measured before and after treatment to evaluate hypoxia-induced stress. Significant differences in the concentrations of sodium between the control groups (treatment 1, normoxia) and the experimental groups (treatment 3, 3 mg 1(-1) O-2) were found in the species A. pallipes and A. torrentium. Groups of A. italicus did not show any significant difference between treatments in sodium concentrations but in chloride concentrations. Crayfish of all three species demonstrated a disruption in the ion exchange process in hypoxia, but all tolerated very low oxygen concentration for an extended period of time

ROUNDTABLE SESSION 4A: MANAGEMENT: REINTRODUCTIONS AND RESTOCKING.

The present paper summarizes the outcome of a roundtable discussion on reintroductions or restocking as a management strategy for native European crayfish, which took place during the European Crayfish conference in Poitiers, France in September 2001. Aspects related to the suitability of the target habitat, the stocking material and the stocking procedure itself are important to consider during any reintroduction measure: Apart from general water quality and structural parameters, a suitable habitat is ideally geographically isolated from other surface waters and human activities such as intensive fishing pressure. However, it is of striking importance to make sure that the target habitat is free of crayfish plague, e.g. by experimental in situ exposure of native specimens. Overexploitation should be avoided if the stocking material originates from other surface waters and genetic aspects should be considered in stocking material originating from both natural surface waters and crayfish farms. Information for the stocking procedure itself are summarized from experiences gathered in various European countries. As an overall result of the discussion, the following three key questions related to reintroductions of native crayfish arised: 1. Why are the native crayfish not present (anymore) in the target habitat? 2. Do native crayfish survive in situ exposure as a test for crayfish plague? 3. Is the reintroduction or restocking measure successful in establishing a self-sustaining stock of native crayfish