Far-field mapping of the longitudinal magnetic and electric optical fields

In this letter, we demonstrate the experimental mapping of the longitudinal magnetic and electric optical fields with a standard scanning microscope that involves a high numerical aperture far-field objective. The imaging concept relies upon the insertion of an azimuthal or a radial polarizer within the detection path of the microscope which acts as an optical electromagnetic filter aimed at transmitting selectively to the detector the signal from the magnetic or electric longitudinal fields present in the detection volume, respectively. The resulting system is thus versatile, non invasive, of high resolution, and shows high detection efficiencies. Magnetic optical properties of physical and biological micro and nano-structures may thus be revealed with a far-field microscope

Micro Balloon Actuators for Aerodynamic Control

A robust, large-force, large-deflection micro balloon actuator for aerodynamic (manoeuvring) control of transonic aircraft has been developed. Using a novel process, high yield linear arrays of silicone balloons on a robust silicon substrate have been fabricated that can deflect vertically in excess of one mm. Balloon actuators have been tested under cyclic conditions to assess reliability. The actuators have been characterized in a wind tunnel to assess their suitability as aerodynamic control surfaces and flight-tested on a jet fighter to assess their resistance to varied temperatures and pressures at high velocity

Scanning reflectance spectroscopy (380-730nm): a novel method for quantitative high-resolution climate reconstructions from minerogenic lake sediments

High-resolution (annual to sub-decadal) quantitative reconstructions of climate variables are needed from a variety of paleoclimate archives across the world to place current climate change in the context of long-term natural climate variability. Rapid, high-resolution, non-destructive scanning techniques are required to produce such high-resolution records from lake sediments. In this study we explored the potential of scanning reflectance spectroscopy (VIS-RS; 380-730nm) to produce quantitative summer temperature reconstructions from minerogenic sediments of proglacial, annually laminated Lake Silvaplana, in the eastern Swiss Alps. The scanning resolution was 2mm, which corresponded to sediment deposition over 1-2years. We found correlations up to r=0.84 (p<0.05) for the calibration period 1864-1950, between six reflectance-dependent variables and summer (JJAS) temperature. These reflectance-dependent variables (e.g. slope of the reflectance 570/630nm, indicative of illite, biotite and chlorite; minimum reflectance at 690nm indicative of chlorite) indicate the mineralogical composition of the clastic sediments, which is, in turn, related to climate in the catchment of this particular proglacial lake. We used multiple linear regression (MLR) to establish a calibration model that explains 84% of the variance of summer (JJAS) temperature during the calibration period 1864-1950. We then applied the calibration model downcore to develop a quantitative summer temperature reconstruction extending back to AD 1177. This temperature reconstruction is in good agreement with two independent temperature reconstructions based on documentary data that extend back to AD 1500 and tree ring data that extend back to AD 1177. This study confirms the great potential of in situ scanning reflectance spectroscopy as a novel non-destructive technique to rapidly acquire high-resolution quantitative paleoclimate information from minerogenic lake sediment

Land-based, closed-cycle echiniculture of <i>Paracentrotus lividus</i> (Lamarck) (Echinoldea: Echinodermata): a long-term experiment at a pilot scale

Today, most sea urchins fisheries worldwide must deal with overexploitation. Better management of exploited field populations and/or aquaculture is increasingly considered necessary to sustain sea urchin production in the future. In this context, we evaluate the potential of land-based, closed-cycle echiniculture. A long-term experiment with the edible sea urchin Paracentrotus lividus has been done on a pilot scale. The process allows total independence from natural resources, because the entire biological cycle of the echinoids is under control (closed-cycle echiniculture), and all activities are performed on land. Furthermore, a method has been set up to control the reproductive cycle with the aim to produce marketable individuals all year long. Performances obtained on each stage of the rearing process are quantified and analyzed. Overall, the results of this experiment are promising; however, some problems remain to be solved before we can claim profitability. The most important finding is that land-based, closed-cycle echiniculture is a potential viable supplement to fisheries to sustain worldwide sea urchin roe production

Consecutive episodes of heart and kidney failure in an "otherwise" healthy young man

Background: Acute renal failure is a rare occurrence in a patient with an unremarkable past medical history and should always lead to an in depth clinical study. The occurrence in the same healthy young subject, of consecutive episodes of heart failure and of acute renal failure is an even rarer event and should prompt diagnostic tests and restrict the diagnostic hypotheses. Case presentation: We present the case of a 28 year-old man who, while waiting to undergo assessment for a mild chronic kidney disease, was diagnosed with decompensated dilated cardiomyopathy and placed on diuretics and β-blockers. After few weeks he developed a non oligoanuric acute renal failure with a slight elevation of serum calcium. Renal biopsy proved suggestive for renal sarcoidosis; thus the hypothesis of systemic sarcoidosis with cardiac and renal involvement was possible avoiding further delay in initiation of therapy. Conclusions: Cardiac sarcoidosis is usually silent but the majority of cases are diagnosed when cardiac symptoms are present in a patient with systemic sarcoidosis. Renal involvement with granulomatous interstitial nephritis is also quite rare and can be an unexpected finding at kidney biopsy. This case highlights the need to evaluate thoroughly clinical problems that do not fit in a specific scenario and emphasizes the importance of performing a kidney biopsy in case of kidney failure of unknown etiology

Improvement of the size estimation of 3D tracked droplets using digital in-line holography with joint estimation reconstruction

International audienceDigital holography is a valuable tool for three-dimensional information extraction. Among existing configurations, the originally proposed setup (i.e. Gabor, or in-line holography), is reasonably immune to variations in the experimental environment making it a method of choice for studies of fluid dynamics. Nevertheless, standard hologram reconstruction techniques, based on numerical light back-propagation are prone to artifacts such as twin images or aliases that limit both the quality and quantity of information extracted from the acquired holograms. To get round this issue, the hologram reconstruction as a parametric inverse problem has been shown to accurately estimate 3D positions and the size of seeding particles directly from the hologram. To push the bounds of accuracy on size estimation still further, we propose to fully exploit the information redundancy of a hologram video sequence using joint estimation reconstruction. Applying this approach in a bench-top experiment, we show that it led to a relative accuracy of 0.13 % (for a 30 µm diameter droplet) for droplet size estimation, and a tracking accuracy of σ x × σ y × σ z = 0.15 × 0.15 × 1 pixels

High-speed Photometric Observations of ZZ Ceti White Dwarf Candidates

We present high-speed photometric observations of ZZ Ceti white dwarf candidates drawn from the spectroscopic survey of bright DA stars from the Villanova White Dwarf Catalog by Gianninas et al., and from the recent spectroscopic survey of white dwarfs within 40 parsecs of the Sun by Limoges et al. We report the discovery of six new ZZ Ceti pulsators from these surveys, and several photometrically constant DA white dwarfs, which we then use to refine the location of the ZZ Ceti instability strip.Comment: 4 pages, 1 table, 2 figures, to appear in "19th European White Dwarf Workshop" in the ASP Conference Serie

MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast

Faithful segregation of chromosomes during cell division relies on multiple processes such as chromosome attachment and correct spindle positioning. Yet mitotic progression is defined by multiple parameters, which need to be quantitatively evaluated. To study the spatiotemporal control of mitotic progression, we developed a high-content analysis (HCA) approach that combines automated fluorescence microscopy with real-time quantitative image analysis and allows the unbiased acquisition of multiparametric data at the single-cell level for hundreds of cells simultaneously. The Mitotic Analysis and Recording System (MAARS) provides automatic and quantitative single-cell analysis of mitotic progression on an open-source platform. It can be used to analyze specific characteristics such as cell shape, cell size, metaphase/anaphase delays, and mitotic abnormalities including spindle mispositioning, spindle elongation defects, and chromosome segregation defects. Using this HCA approach, we were able to visualize rare and unexpected events of error correction during anaphase in wild-type or mutant cells. Our study illustrates that such an expert system of mitotic progression is able to highlight the complexity of the mechanisms required to prevent chromosome loss during cell division

A rapid microwave-assisted procedure for easy access to Nx polydentate ligands for potential application in α-RIT

International audienceHeterocycles bearing a hydrazine moiety react with bisaldehydes or bisketones to afford new Nx polydentate ligands suitable for α-radioimmunotherapy. We developed a fast and efficient method using microwave-assisted technology to obtain chelators with variable size and number of coordination centres which were fully characterized. The complexation efficiency with astatine will be assessed