672 research outputs found
Physical activity and late effects in childhood acute lymphoblastic leukemia long-term survivors
Glutathione limits Ero1-dependent oxidation in the endoplasmic reticulum
Many proteins of the secretory pathway contain disulfide bonds that are essential for structure and function. In the endoplasmic reticulum (ER), Ero1alpha and Ero1beta oxidize protein disulfide isomerase (PDI), which in turn transfers oxidative equivalents to newly synthesized cargo proteins. However, oxidation must be limited, as some reduced PDI is necessary for disulfide isomerization and ER-associated degradation. Here we show that in semipermeable cells, PDI is more oxidized, disulfide bonds are formed faster, and high molecular mass covalent protein aggregates accumulate in the absence of cytosol. Addition of reduced glutathione (GSH) reduces PDI and restores normal disulfide formation rates. A higher GSH concentration is needed to balance oxidative folding in semipermeable cells overexpressing Ero1alpha, indicating that cytosolic GSH and lumenal Ero1alpha play antagonistic roles in controlling the ER redox. Moreover, the overexpression of Ero1alpha significantly increases the GSH content in HeLa cells. Our data demonstrate tight connections between ER and cytosol to guarantee redox exchange across compartments: a reducing cytosol is important to ensure disulfide isomerization in secretory proteins
Successful Hematopoietic Stem Cell Transplantation in a Patient with Complete IFN-γ Receptor 2 Deficiency: a Case Report and Literature Review
Continuous antibiotic infusion for salvage therapy of partially implanted central venous catheter tunnel infections due to staphylococci
Multipotent mesenchymal stem cells from amniotic fluid originate neural precursors with functional voltage-gated sodium channels
Avaliação de genótipos de trigo irrigado para panificação e macarrão, em Minas Gerais, no ano de 2007.
bitstream/CNPT-2010/40335/1/p-bp62.pd
Long-term effect of anticancer therapy on dentition of Italian children in remission from malignant disease: A cross-sectional study
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