Transcripcional, functional and virulence analysis of a Pseudomonas Savastanoi pv. savastanoi genomic region shared with other pathogens of woody hosts

Comunicación al XVI congreso de la Sociedad Internacional de Interacciones moleculares Planta-PatógenoThe genome of the olive tree pathogen Pseudomonas savastanoi pv. savastanoi (Psv) NCPPB3335 (58.1% G+C) encodes a region of about 15 kb, named VR8 (60.4% G+C), which is absent in all sequenced Pseudomonas syringae strains infecting herbaceous plants, but shared with P. syringae pathovars infecting woody hosts. RT-PCR analysis of the VR8 genes revealed the existence of 4 possible operons, of which the antABC and catBCA operons are involved in the degradation of anthranilate and catechol, respectively. The antABC cluster is homologous to the anthranilate degradation genes found on plasmid pCAR1 of Pseudomonas resinovorans. The other two operons, here called AER-1901/2/3 and AER-1904/5, also show homology to genes related with the degradation of aromatic compounds. RT-qPCR and ß-galactosidase assays of a LacZ fusions showed that both anthranilate and 6-chloro-antrhanilate induce the antABC operon. In addition, anthranilate also induces the catB gene. To analyse the role in virulence of the VR8, we constructed several knockout mutants of this region. The volume of the knots induced in non lignified olive plants by Psv mutants affected in the antABC or catBCA operons resulted to be similar to those induced by the wild-type strain. However, the severity of the symptoms generated by the antABC mutant in lignified olive plants was significantly lower than that induced by the wildtype strain, suggesting a possible role of this operon in the degradation of lignin-derived compounds. At present, we are analysing the role in virulence of the other three operons and of the AER-1900 gene (a putative aerotaxis receptor).Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Psychometric properties of the Liebowitz Social Anxiety Scale in a large cross-cultural Spanish and Portuguese speaking sample

[EN]Two groups of participants were included: a non-clinical sample involving 31,243 community subjects and a clinical sample comprising 529 patients with a diagnosis of social anxiety disorder (SAD). Exploratory factor analysis (EFA), confirmatory factor analysis (CFA) and exploratory structural equation modeling (ESEM) were used in order to determine the psychometric properties of the LSAS-SR. EFA identified five factors with eigenvalues greater than 1.00 explaining 50.78% of the cumulative variance. CFA and ESEM supported this 5-factor structure of the LSAS-SR. The factors included: 1) speaking in public; 2) eating/drinking in front of other people; 3) assertive behaviors; 4) working/writing while being observed; and 5) interactions with strangers. Other psychometric properties such as inter-factor correlations, invariance, reliability, and validity of the scale were also foun

Aldehyde Dehydrogenase 3 Is an Expanded Gene Family with Potential Adaptive Roles in Chickpea

Legumes play an important role in ensuring food security, improving nutrition and enhancing ecosystem resilience. Chickpea is a globally important grain legume adapted to semi-arid regions under rain-fed conditions. A growing body of research shows that aldehyde dehydrogenases (ALDHs) represent a gene class with promising potential for plant adaptation improvement. Aldehyde dehydrogenases constitute a superfamily of proteins with important functions as ‘aldehyde scavengers’ by detoxifying aldehydes molecules, and thus play important roles in stress responses. We performed a comprehensive study of the ALDH superfamily in the chickpea genome and identified 27 unique ALDH loci. Most chickpea ALDHs originated from duplication events and the ALDH3 gene family was noticeably expanded. Based on the physical locations of genes and sequence similarities, our results suggest that segmental duplication is a major driving force in the expansion of the ALDH family. Supported by expression data, the findings of this study offer new potential target genes for improving stress tolerance in chickpea that will be useful for breeding programs

Disolventes nanoestructurados para la microextracción de astaxantina y cantaxantina en productos de piscifactoría

III Encuentro sobre Nanociencia y Nanotecnología de Investigadores y Tecnólogos Andaluce

La autoestima y su relación con la ansiedad social y las habilidades sociales

Este trabajo examina la autoestima y sus dimensiones utilizando la “Escala Janis-Field de sentimientos de inadecuación-Revisada” (RJFFIS) y la “Escala Rosenberg de autoestima” (RSES) en una muestra mayoritariamente universitaria (n= 826). Analizamos su relación con la ansiedad social (AS) y las habilidades sociales (HHSS), evaluadas con el “Cuestionario de ansiedad social para adultos” (CASO), el “Cuestionario de habilidades sociales” (CHASO) y el “Inventario de asertividad de Rathus” (RAS). La solución heptafactorial de la RJFFIS explica el 59, 65% de la varianza común y apoya un concepto multifactorial de la autoestima, incluyendo facetas que tienen que ver con la apariencia y habilidades físicas, relaciones sociales y habilidades académicas o laborales. Las correlaciones moderadas con la RSES apoyan la validez convergente de la RJFFIS. Por otra parte, la autoestima se relaciona de forma moderada con la AS y las HHSS. Los sujetos con alta AS muestran una autoestima significativamente más baja que aquellos con baja AS y lo opuesto ocurre respecto a las HHSS. Existen diferencias de sexo en cuanto a la autoestima, mostrando los hombres puntuaciones más altas que las mujeres. Estos resultados sustentan la naturaleza multidimensional de la autoestima y la necesidad de investigar su papel en las áreas tanto de la AS como de las HHSS. This paper examines self-esteem and its dimensions using the Revised Janis-Field Feelings of Inadequacy Scale (RJFFIS) and the Rosenberg Self-Esteem Scale (RSES) in a sample comprising mostly university undergraduates (n= 826). We analyzed their relationship with social anxiety (SA) and social skills (SS), assessed with the Social Anxiety Questionnaire for adults (SAQ), the Social Skills Questionnaire (CHASO) and the Rathus Assertiveness Schedule (RAS). The hepta-factorial solution for the RJFFIS explains 59.65% of the common variance and supports a multifactorial concept of self-esteem, including facets that have to do with physical appearance and abilities, social relationships, and academic or work skills. The moderate correlations with the RSES support the convergent validity of the RJFFIS. On the other hand, self-esteem is moderately related to SA and SS. Subjects with high SA show significantly lower self-esteem than those with a low SA and the opposite occurs regarding SS. There are gender differences in terms of self-esteem, with men showing higher scores than women. These results support the multidimensional nature of self-esteem and the need to investigate its role in the areas of both SA and SS

The Northern ROSAT All-Sky (NORAS) Galaxy Cluster Survey I: X-ray Properties of Clusters Detected as Extended X-ray Sources

In the construction of an X-ray selected sample of galaxy clusters for cosmological studies, we have assembled a sample of 495 X-ray sources found to show extended X-ray emission in the first processing of the ROSAT All-Sky Survey. The sample covers the celestial region with declination $\delta \ge 0\deg$ and galactic latitude $|b_{II}| \ge 20\deg$ and comprises sources with a count rate $\ge 0.06$ counts s$^{-1}$ and a source extent likelihood of 7. In an optical follow-up identification program we find 378 (76%) of these sources to be clusters of galaxies. ...Comment: 61 pages; ApJS in press; fixed bug in table file; also available at (better image quality) http://www.xray.mpe.mpg.de/theorie/NORAS