Living Bacterial Sacrificial Porogens to Engineer Decellularized Porous Scaffolds

Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques suffer from limited control over pore interconnectivity, density and size, which leads to inefficient nutrient and oxygen transport to cells embedded in the scaffolds. Here, we demonstrated an innovative approach to develop a new platform for tissue engineered constructs using live bacteria as sacrificial porogens. E.coli were patterned and cultured in an interconnected three-dimensional (3D) hydrogel network. The growing bacteria created interconnected micropores and microchannels. Then, the scafold was decellularized, and bacteria were eliminated from the scaffold through lysing and washing steps. This 3D porous network method combined with bioprinting has the potential to be broadly applicable and compatible with tissue specific applications allowing seeding of stem cells and other cell types

Face To Face Proximity Estimation Using Bluetooth on Smartphones

ABSTRACT The availability of "always-on" communications has tremendous implications for how people interact socially. In particular, individuals within a certain distance? Moreover, the problem of proximity estimation is complicated by the fact that the measurement must be quite precise (1-1.5 m) and can cover a wide variety of environments. Existing approaches such as GPS and Wi-Fi triangulation are insufficient to meet the requirements of accuracy and flexibility. In contrast, Bluetooth, which is commonly available on most smartphones, provides a compelling alternative for proximity estimation. In this paper, we demonstrate through experimental studies the efficacy of Bluetooth for this exact purpose. We propose a proximity estimation model to determine the distance based on the RSSI values of Bluetooth and light sensor data in different environments. We present several real world scenarios and explore Bluetooth proximity estimation on Android with respect to accuracy and power consumption