Micropropagation and Assessment of Genetic Stability of Acclimatized Streptocarpus x hybridus Voss Plantlets Using RAPD Markers

A micropropagation protocol via direct shoot organogenesis from Streptocarpus x hybridus Voss. leaf explants was established in this study. The shoot induction of three Streptocarpus cultivars (‘Snow White’, ‘Black Panther’ and ‘Slumber Song’) was successfully achieved on Murashige and Skoog (MS) medium supplemented with 0.2 mg L-1 -indole-3-acetic acid (IAA) and 0.2 mg L-1 thidiazuron (TDZ). In proliferation stage, the effects of two combinations of plant growth regulators -PGR- (V1-0.2 mg/L-1 IAA + 0.5 mg/L-1 BAP and V2-1.0 mg L-1 NAA + 0.2 mg L-1 TDZ) on shoot number and length were examined. The results suggest that PGRs combinations significantly influenced shoot proliferation and root induction in all Streptocarpus cultivars. Among the treatments, 0.2 mg L-1 (IAA) in combination with 0.5 mg L-1 6-benzylaminopurine (BAP) were the most effective for in vitro shoot multiplication and rooting. The in vitro rooting percentage was also determined before subjecting the plantlets to the acclimatization process. Due to acclimatization, Streptocarpus plantlets showed a very high rate of survival (90%). The generated PCR-RAPD profiles for the selected in vitro-raised plants and donor plants were similar which indicates the clonal or true-to-type nature of the progenies

SINUS LIFT USING A-PRF AND CERABONE AND SIMULTANEOUS INSERTION OF IMPLANTS – CASE REPORT

Sinus floor elevation with autogenous bone grafts and/or bone substitutes is a generally accepted procedure that allows the insertion of implants. Recent studies have shown good results of the use of the PRF in stimulating bone regeneration, especially when it is used in combination with other grafting materials. The purpose of this case report is to present the clinical results of sinus lift procedure through the lateral window antrostomy in the right sinus using A-PRF and bone substituents (Cerabone) and simultaneous insertion of implants as well as the evaluation of healing time. The results of this case report shows that A PRF can be used successfully in combination with bone substitutes in lateral sinus lift technique with immediate insertion of implants. All implants that were inserted immediately, simultaneously with sinus lift procedure were osteointegrated correctly and it was possible to proceed to the stage of final prosthesis. Conclusions: The use of the combination of A-PRF and Cerabone in sinus lift technique speeded healing time by approximately 50%, thus favoring implant osseointegration, went without postoperative complications and showed good acceptance by the patient

THE MANAGEMENT OF PERIAPICAL MAXILLARY CYST BY USING THE A-PRF (PLATELET RICH ADVANCED FIBRIN) A CASE REPORT

Periapical or radicula cysts are the most common inflammatory cysts of the jaw. The surgical intervention aims to remove periapical pathology to obtain bone regeneration and healing of periapical tissues. Improving the regeneration of the human body by using the the patient's own blood is a unique concept in dentistry. The purpose of this case report is to illustrate the effectiveness of advanced platelet-rich fibrin (A-PRF) inserted into the bone defect resulting from a periapical cyst enucleation. The physiological time of healing of the cystic cavity is from 6 months to 1 year, but when the cystic cavity is filled with A-PRF, this phenomenon of physiological healing is accelerated, the healing period decreasing to three months. The results of this case report shows that APRF can be used successfully as monotherapy for obtaining periapical regeneration

TREATMENT OF MULTIPLE ADJACENT GINGIVAL RECESSIONS THROUGH THE MODIFIED TUNNEL TECHNIQUE USING CONNECTIVE TISSUE GRAFT

Aim of the study One of the most commonly used esthetic periodontal procedures is the coverage of denuded roots. Correction of gingival recession is an important issue in the field of periodontal plastic surgery. The purpose of this study is to evaluate the efficacy and clinical results of the use of connective tissue grafts harvested from the palate in the treatment of Miller Class I and II localized gingival recession through modified tunnel technique. Material and methods The study population consisted of 17 patients with multiple adjacent gingival recession that required gingival augmentation. It was recommended the modified tunnel technique that uses tissue graft harvested from the palate. The etiologic factor of the lesions was aggressive tooth brushing. Results The healing process was uneventful and much more quickly due to lack of incisions. The average coverage of the recessions was 2.3 ± 0.5 mm. The color of tissues was nearly homogeneous at 2 weeks. Conclusions Due to its minimally invasive approach, this technique speeds up the healing process and generate more aesthetic results, it is more easily accepted by patients, ensuring their satisfaction

PROSTHETIC REHABILITATION USING IMPLANTS AFTER ENUCLEATION OF AN EXTENSIVE MANDIBULAR MULTILOCULAR DENTIGEROUS CYST: A CASE REPORT

Dentigerous cysts are the most common odontogenic developmental cyst being associated to an impacted tooth. They are usually asymptomatic and are discovered on routine radiographic examinations performed in order to identify the absence of tooth on the arch. The purpose of this case report is to present the spontaneous recovery of a bone defect without the need for bone addition material, as a result of enucleation of large mandibular multilocular dentigerous cyst together with the impacted tooth and the subsequent prosthetic rehabilitation of partial edentulism with dental implants. After 6 months after cystectomy, at the radiographic examination (OPG and CT) was noted the disappearance of almost all of the radiolucency and the formation of a sufficient quantity of bone for the insertion of the implants.They had good primary stability. By loading the implants and the fixed partial denture, functional, physiognomic and psychological needs of the patient have been fulfilled successfully, not being detected the cyst recurrence. Prosthetic rehabilitation with dental implants was the best solution in order to replace the missing teeth in case of an adequate bone regeneration

Studies on mobile robots for all types of terrain

In the first part of the paper, the authors present the characteristics of the robots for all types of terrain. In the second part, two categories of robots are proposed: a robot with hybrid locomotion system and a modular robot. For the last category, if different modules are combined, a family of modular robots adaptable to different types of terrain can be obtained. The solutions proposed by the authors allow the study of the mobility and adaptability of robots to different types of terrain

THE PHYSIOGNOMY RESTORATION IN UPPER ANTERIOR MAXILLARY REGION USING A SINGLE IMPLANT BREDENT

The purpose of this study was to evaluate the features, results and complications of the surgical procedure in a single step of immediate insertion of a single implant after atraumatic extraction of a single tooth in the maxillary anterior region. Material and methods In 21 patient was made the insertion of an immediate postextraction implant, an immediate temporary reconstruction of the breach and subsequent realization of a metalceramic crowns on implant. Results A single implant was not integrated. Clinical and radiographic parameters were good. One provisional crown was fractured. At the postoperative control carried out at 6 months it was found that implants were stable without bone loss at radiological exam, the interdental papillae were present. Conclusions Single implant placement after extraction in the maxillary aesthetic zone proved to be a procedure with a high acceptability and satisfaction from the patients. Uploading a single implant in the socket immediately after extraction shortens the total treatment time, fewer surgical interventions, preserves the structure of adjacent teeth