Importance of microscopic testing of honey and pollen samples in the prophylaxis of major bacterial diseases in apis mellifera carpathica bees

The purpose of the study was to monitor the presence of bacilli in the honey and pollen samples in correlation to the positive diagnosis of these major bacterial diseases in bees. The study took 3 years, and approximately 156 samples of honey and bee bread from reserve honeycombs and 156 live bee intestine samples were processed. To identify the bacilli in honey, bee bread (pollen) reserve and live bees intestine , we used our own method, and the confirmation of their presence was done through methodology OIE/2008. Of the total tested samples, the bacilli were found present in 63 samples from reserve honeycombs and in 67 samples from live bees’ intestine. The bee colonies that did not test bacilli in the samples examined for the duration of the monitoring, did not present a disease episode and did not register mortality of pathologic nature. The mortality registered in the apiaries under study throughout the 3 year-period was 30-100 % for the apiares from which samples testing positive for bacilli had been received. The study confirms that a correlation exists between the presence of bacilli in samples of honey and bee bread from reserve honeycombs, and their presence in adult bees’ intestine. The microscopic testing of honey and pollen samples, as well as of bee intestine, may constitute an important prophylactic method in the management of major bacterial diseases in bees (American and European foulbrood)

The prophylaxis of major bacterial infections in the Apis mellifera carpathica bee through honey, pollen and bee bread control

For the purpose of controlling the evolution of major bacterial diseases in bees, which decimate bee colonies in Europe and Romania, respectively, we examined samples (honey, pollen and honeycombs) in the apicultural year 2016, from all over Romania. Sample collection and testing were done with the purpose to prevent the contamination of bee colonies with the etiological agents of major bacterial diseases, considering that worker bees and the food entering the hive (honey, pollen) represent the main contamination ways. The diagnosis method observed OIE regulations (2008) and was adapted in an original way in the Bee Pathology Laboratory in Bucharest. A total of 73 samples were examined, representing honey (51), honeycombs (6) and pollen/bee bread (16), from private apiaries all over the country, that presented depopulation without clinical evolution of contagious diseases in bees, and in which we diagnosed the presence of etiological agents of major bacterial bee diseases (36.98 %), while the rest of the samples were negative (63.02%). Of the 51 samples of honey that were examined, we identified 39.22% positive samples and 60.78% negative ones. Of the pollen samples that were examined, 31.25% were positive and 68.75% were negative, and the honeycombs samples showed 33.33% positive and 66.66% negative. Previous researches indicated that the positive samples (honey, pollen, bee bread), from apiaries in all the regions of the country, represented the basis for the prophylaxis of major bacterial diseases so that, by avoiding using them in bee nutrition, the evolution of major bee diseases did not confirm clinically or paraclinically in the following season (January-April 2017)

Novel Reinforcement Learning Research Platform for Role-Playing Games

The latest achievements in the field of reinforcement learning have encouraged the development of vision-based learning methods that compete with human-provided results obtained on various games and training environments. Convolutional neural networks together with Q-learning-based approaches have managed to solve and outperform human players in environments such as Atari 2600, Doom or StarCraft II, but the niche of 3D realistic games with a high degree of freedom of movement and rich graphics remains unexplored, despite having the highest resemblance to real-world situations. In this paper, we propose a novel testbed to push the limits of deep learning methods, namely an OpenAI Gym-like environment based on Dark Souls III, a notoriously difficult role-playing game, where even human players have reportedly struggled. We explore two types of architectures, Deep Q-Network and Deep Recurrent Q-Network, providing the results of a first incursion into this new problem class. The source code for the training environment and baselines is made available

Novel Reinforcement Learning Research Platform for Role-Playing Games

The latest achievements in the field of reinforcement learning have encouraged the development of vision-based learning methods that compete with human-provided results obtained on various games and training environments. Convolutional neural networks together with Q-learning-based approaches have managed to solve and outperform human players in environments such as Atari 2600, Doom or StarCraft II, but the niche of 3D realistic games with a high degree of freedom of movement and rich graphics remains unexplored, despite having the highest resemblance to real-world situations. In this paper, we propose a novel testbed to push the limits of deep learning methods, namely an OpenAI Gym-like environment based on Dark Souls III, a notoriously difficult role-playing game, where even human players have reportedly struggled. We explore two types of architectures, Deep Q-Network and Deep Recurrent Q-Network, providing the results of a first incursion into this new problem class. The source code for the training environment and baselines is made available

Serum Interleukins 8, 17, and 33 as Potential Biomarkers of Colon Cancer

This research investigated the serum levels of three interleukins (IL8, IL17A, and IL33) and the possible relationships between them in healthy people and colon cancer patients at different stages. This study involved 82 participants, 42 of whom had colon cancer and 40 were healthy individuals. The cancer patients were classified into four groups according to the TNM staging classification of colon and rectal cancer. Serum levels of the interleukins were measured by the ELISA test. The data were analyzed statistically to compare the demographic characteristics, the interleukin levels across cancer stages, and the correlation between interleukins in both groups. The results showed that women had more early-stage colon cancer diagnoses, while men had more advanced-stage cancer diagnoses. Stage two colon cancer was more common in older people. Younger people, men, and those with early-stage colon cancer had higher levels of interleukins. The levels of IL8 and IL17A were higher in the cancer group, while the level of IL33 was higher in the healthy group. There was a strong correlation between IL8 and IL17A levels in both groups (p = 0.001). IL17A influenced the level of IL33 in the cancer group (p = 0.007). This study suggested that cytokine variation profiles could be useful for detecting colon cancer and predicting its outcome

Specific Septic Complications after Rectal Cancer Surgery: A Critical Multicentre Study

The postoperative septic complications in gastrointestinal surgery impact immediate as well as long-term outcomes, which lead to reinterventions and additional costs. The authors presented the experience of three surgery clinics in Romania regarding the specific septic complications occurring in patients operated on for rectal cancer. The study group comprised 2674 patients who underwent surgery over a 5-year period (2017–2021). Neoplasms of the middle and lower rectum (76%) were the majority. There were 85% rectal resections and 15% abdominoperineal excisions of the rectum. In total, 68.54% of patients were operated on laparoscopically, and 31.46% received open surgery. Without taking wound infections into account, 97 (3.67%) patients had abdominal-pelvic septic complications. The aim was to evaluate the causes of the complications. The percentage of suppurations after surgery of the rectum treated by radiochemotherapy was considerably higher than after surgery of the non-radiated upper rectum. The fatality rate was 5.15%. The risk of fistulas was significantly associated with the preoperative treatment, tumour position and type of intervention. Sex, age, TNM stage or grade were not significant at 0.05 the threshold. The risk of fistulas is reduced with low anterior resection, but the gravity of these complications is higher in the lower rectum compared with the superior rectum. Preoperative radiochemotherapy is a contributing factor to septic complications