Inhibition of poly(ADP-ribose)polymerase binding to DNA by thymidine dimer

AbstractThe ability of poly(ADP-ribose)polymerase to bind damaged DNA was assessed by electrophoretic mobility shift assay. DNA binding domain of poly(ADP-ribose)polymerase (PARPDBD) binds to synthetic deoxyribonucleotide duplex 10-mer. However, the synthetic deoxyribonucleotide duplex containing cys-syn thymidine dimer which produces the unwinding of DNA helix structure lost its affinity to PARPDBD. It was shown that the binding of PARPDBD to the synthetic deoxyribonucleotide duplex was not affected by O6-Me-dG which causes only minor distortion of DNA helix structure. This study suggests that the stabilized DNA helix structure is important for poly(ADP-ribose)polymerase binding to DNA breaks, which are known to stimulate catalytic activity of poly(ADP-ribose)polymerase

Effects of Chromium Methionine Supplementation on Blood Metabolites and Fatty Acid Profile of Beef during Late Fattening Period in Holstein Steers

The objective of this study was to determine the effects of chromium methionine (Cr-Met) chelate supplementation on blood metabolites and fatty acid profile of beef from Holstein steers during late fattening period. Fifteen Holstein steers were allotted randomly into two groups including the control (non Cr-Met feeding, NCM, ave. body weight [BW] = 483±25.7 kg) and the treatment (Cr-Met feeding for 4 months, 4CM, ave. BW = 486±27.5 kg) group. The feeding amount of Cr-Met to animals was limited to 400 ppb/cow/d and was supplemented to total mixed ration. No difference in blood albumin, alkaline phosphatase, urea-nitrogen, calcium, creatine, glucose, total protein, triglyceride, and cholesterol were observed between the treatment groups (p>0.05). The level of high density lipoprotein was higher in the 4CM group than the NCM group, whereas low density lipoprotein was lower in the 4CM group (p0.05). The arachidonic acid level tended to be higher in the 4CM than the NCM group (p = 0.07). Cr-Met had no influence (p>0.05) on the ratio of saturated, unsaturated, unsaturated/saturated, monounsaturated/saturated and polyunsaturated/saturated fatty acids whereas the ratio of polyunsaturated fatty acids (PUFA) in the 4CM group was comparatively higher than the NCM group (p<0.05). This study concluded that feeding Cr-Met supplementation in 400 ppb/d to Holstein steers for 4 months during late fattening period can improve some blood metabolites and beef quality by increasing PUFA and gamma-linoleate compositions of beef

Living donor liver transplantation for an adult patient with situs inversus totalis

This recipient with situs inversus totalis (SIT) was a 60-year-old female who had hepatitis B-related end-stage liver disease. Preoperative donor evaluation showed that the right posterior section satisfied graft volume and was space-fitting in the recipient hepatic fossa when it was rotated 180 degrees. The operation and postoperative course progressed satisfactorily. Three weeks after living donor liver transplantation (LDLT), the graft function was disturbed by compression of bottom-placed right hepatic vein. This was treated with a vascular stent and subsequently the graft function was normalized. The present case shows that LDLT for patients with SIT using a right posterior section graft is feasible

Effects of water addition to total mixed ration on water intake, nutrient digestibility, wool cortisol and blood indices in Corriedale ewes

Objective The objective of this study was to determine the effect of adding water to total mixed ration (TMR) on fresh water intake, nutrient digestibility, wool cortisol, and blood indices in Corriedale ewes under hot and humid conditions. Methods Nine non-pregnant Corriedale ewes (ave. body weight = 41±3.5 kg) were individually fed diets based on maintenance requirements in metabolic crates. Ewes were assigned to three treatment groups according to a triplicate 3×3 Latin Square design for 3 periods of 21 days duration each (9 ewes per treatment, 27 replications). Treatments were TMR (crude protein [CP] = 16.1, total digestible nutrients = 69.1%) moisture levels for 40%, 50%, and 60%. Results No differences were found in body weight gain among all treatment groups (p>0.05). Nitrogen balance including digestible N, retained N, and urinary and fecal N showed no change among the treatment groups (p>0.05). Fresh water intake was the lower in 50% TMR moisture group than in the other groups (p0.05). No significant difference was observed for serum protein, blood urea nitrogen, glucose, and triglyceride among the treatment groups (p>0.05). Wool and blood cortisol were not different among the treatment groups (p>0.05). Blood hematology including red blood cell, white blood cells, hemoglobin, hematocrit, basophils, and eosinophils were not different among the treatment groups (p>0.05). Conclusion It is concluded that TMR moisture at 40%, 50%, and 60% had no effects on N balance parameters, and nutrient digestibilities except for the ether extract under hot and humid conditions. Additionally there were no effects on stress conditions include wool cortisol, as well as blood cortisol levels of ewes

Fatty acid profile of meat, diurnal changes in volatile fatty acids, rumen fluid parameters, and growth performance in Korean native (Hanwoo) steers fed high- and low-forage diets supplemented with chromium-methionine

ABSTRACT The objective of this study was to determine the effects of forage level in diets supplemented with chromium-methionine (Cr-Met) on rumen fluid parameters, meat fatty acid composition, and performance of Korean beef (Hanwoo) steers. Twenty-three Hanwoo steers were used in this experiment. A completely randomized design and repeated measurements were used to analyze the data set. Beef steers were fed diets containing high (10 head; average body weight (BW) = 525.1±27.5; forage:concentrate (F:C) = 60:40) (60F) and low (13 head; average BW = 531.8±32.4; F:C = 40:60 ratio) (40F) forage diets supplemented with Cr-Met for 60 d. Dry matter intake, BW, and feed efficiency were not different between the two treatment groups. Fatty acid composition of meat including myristate, stearate, and gamma linoleate was not different between the two groups; however, palmitate, palimtoleate, and linoleate were higher in 60F group than 40F group. Ammonia-N showed a higher trend in 40F group, whereas pH demonstrated higher values in 60F group. Ruminal acetate was higher in 60F group than 40F group and maintained a high trend throughout the sampling time, whereas no differences were found in ruminal propionate, butyrate, and valerate between two groups. A high-forage diet (60%) improves meat quality and has no adverse effects on performance of Hanwoo steers

Effect of substrate bias on deposition behaviour of charged silicon nanoparticles in ICP-CVD process

The effect of a substrate bias on the deposition behaviour of crystalline silicon films during inductively coupled plasma chemical vapour deposition (ICP-CVD) was analysed by consideration of non-classical crystallization, in which the building block is a nanoparticle rather than an individual atom or molecule. The coexistence of positively and negatively charged nanoparticles in the plasma and their role in Si film deposition are confirmed by applying bias voltages to the substrate, which is sufficiently small as not to affect the plasma potential. The sizes of positively and negatively charged nanoparticles captured on a carbon membrane and imaged using TEM are, respectively, 2.7–5.5 nm and 6–13 nm. The film deposited by positively charged nanoparticles has a typical columnar structure. In contrast, the film deposited by negatively charged nanoparticles has a structure like a powdery compact with the deposition rate about three times higher than that for positively charged nanoparticles. All the films exhibit crystallinity even though the substrate is at room temperature, which is attributed to the deposition of crystalline nanoparticles formed in the plasma. The film deposited by negatively charged nanoparticles has the highest crystalline fraction of 0.84