Identification of Microsporum canis in cutaneous lesions of cats from Timis County

Dermatophytosis has a practical and medical importance both in the veterinary and human medicine due to its zoonotic potential causing economic problems worldwide. The aim of this study was to identify the etiological agents involved in the appearance of cutaneous lesions. 43 cats were examined, from eight locations in the Timis County, with or without cutaneous lesions at the age of 1 month to 11 years. 37 cats belonged to the European breed, one Birmanese breed, three were Persian and two were British Shorthairs. Samples of hair, squamae and crusts were collected from every animal and placed in Petri plates. Direct microscopic examination of hairs, squamae and crusts was done using the slide and coverslip method, with lactophenol and it was examined using the x10 objective. The sample were cultivated on Sabouraud agar gel and DTM (Dermatophyte test). Microsporum canis was the only one species indentified in the cutaneous lesions (group 1) in 35% out of examinated cats (7/20). In group 2 (asymptomatic cats), the only species identified was Microsporum canis in one individual (1/23 respectively 4.37%)

Chemoenzymatic synthesis of new aromatic esters of mono-and oligosaccharides

An efficient and convenient chemoenzymatic route for the synthesis of novel phenolic mono-, di-and oligosaccharide esters is described. Acetal derivatives of glucose, sucrose, lactose and inulin were obtained by chemical synthesis. The fully characterized pure sugar acetals were subjected to enzymatic esterification with 3-(4-hydroxyphenyl) propionic acid (HPPA) in the presence of Novozyme 435 lipase as a biocatalyst. The aromatic esters of alkyl glycosides and glucose acetal were obtained with good esterification yields, characterized by mass spectrometry (MALDI-TOF MS), infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (1H NMR,13C NMR). The synthesis of aromatic esters of disaccharide acetals was successful only for the enzymatic esterification of sucrose acetal. The new chemoenzymatic route allowed the synthesis of novel aromatic esters of inulin as the inulin monoacetal monoester and diester and the inulin diacetal monoester with a polymerization degree of two, as well as the inulin monoacetal monoester with a degree of polymerization of three, were obtained by enzymatic acylation of inulin acetals with HPPA. These compounds could represent a new class of sugar ester surfactants with enhanced bioactivity, antioxidative and antimicrobial properties and with potential application in drug delivery systems.</p

Rutin Linoleate Triggers Oxidative Stress-Mediated Cytoplasmic Vacuolation in Non-Small Cell Lung Cancer Cells

Lung cancer (LC) represents one of the most prevalent health issues globally and is a leading cause of tumor-related mortality. Despite being one the most attractive compounds of plant origin due to its numerous biological properties, the therapeutic applications of rutin (RUT) are limited by its disadvantageous pharmacokinetics. Thus, the present study aimed to evaluate in vitro the application of two RUT fatty acids bioconjugates, rutin oleate (RUT-O) and rutin linoleate (RUT-L), as potential improved RUT-based chemotherapeutics in non-small cell lung cancer (NSCLC) treatment. The results indicate that both compounds lacked cytotoxic potential in EpiAirway™ tissues at concentrations up to 125 µM. However, only RUT-L exerted anti-tumorigenic activity in NCI-H23 NSCLC cells after 24 h of treatment by reducing cell viability (up to 47%), proliferation, and neutral red uptake, causing cell membrane damage and lactate dehydrogenase (LDH) leakage, affecting cytoskeletal distribution, inducing cytoplasmic vacuolation, and increasing oxidative stress. The cytopathic effects triggered by RUT-L at 100 and 125 µM are indicators of a non-apoptotic cell death pathway that resembles the characteristics of paraptosis. The novel findings of this study stand as a basis for further investigations on the anti-cancer properties of RUT-L and their underlying mechanisms