Research Notes : Inheritance of presence/absence of flavonoid compounds in soybean seedcoats

In soybean plants carrying the gene T and having black or brown pigmentation of seed coats, there are numerous compounds that can be detected by thin layer chromatography (TLC). There are four spots (A, B, C and D) that appear to be related; they are yellow-orange under visible light and they fluoresce yellow-orange (duller than quercetin) under UV light after spray-ing with flavone reagent. Phenotypic positions of A and D on 2-way plates are given in Table 1. A is present in all material tested, but D varies in presence/absence (B and C also vary but have not been studied)

Research Notes : Genetics of black pigmentation of soybean seedcoats/hila

Gene T is involved in black pigmentation and w1 in the presence of t is involved in imperfect black pigmentation (Bernard and Weiss, 1973). Tis a phenolase gene resulting in quercetin formation and brown pubescence (Buttery and Buzzell, 1973); W is a flower and hypocotyl-color gene (Hartwig and Hinson, 1962). Thin layer chromatography shows two major \u27spots\u27 involved in black pigmentation of soybean seed coats

Research notes: Soybean parental lines

Seed of five improved breeding lines (Table 1) is available upon request for use in crosses or experimental work. Disease reactions are given in Table 2 and physiological attributes in Table 3. The lines are somewhat improved over the unadapted parents; however, only OX610I has been yield tested

Research Notes : Flavonol classes of cultivars in Maturity Groups 00-IV

Since the complementary action of Fg1 and Fg3 in producing kaempferol 2G-glucosyl-gentiobioside (Buttery and Buzzell, 1975) is associated with deleterious effects on chlorophyll concentration, photosynthetic rate and yield (Buttery and Buzzell, 1976), the bringing together of these two genes in crosses may necessitate selection against the Fg1-Fg3-genotype in the segregating material. For example, with the cross of \u27Corsoy\u27 (Fg1 fg3) x \u27Hawkeye\u27 (fg1 Fg3) at Iowa State University, visual selection was carried out against chlorophyll deficient types during inbreeding in order to develop lines for a physiological study. At the time of selection it was not known that flavonol-glycoside genes were involved. Advanced lines were later classified using thin layer chromatography (Buttery and Buzzell, 1973

Research Notes: Agriculture Canada and United States Department of Agriculture

In an increase plot of foundation seeds of Harosoy in 1957 at Urbana, a number of Harosoy-type plants were found with flowers of a deeper red than the normal purple (P). The color is best described as magenta (M). This mutant was added to the Genetic Type Collection as T235

Research Notes: Determinate-Dt2 Effects on Soybean Characteristics.

Bernard (1972) studied a gene, Dt2, which hastened the termination of apical stem growth and decreased both plant height and number of nodes per plant. In a \u27Harosoy\u27 background, a Dt2 isoline had a 15% reduction in height and was three days earlier maturing but was similar in yield to Harosoy . There was some reduction in weight per seed associated with the Dt2 effect

Research Notes: Agriculture Canada

Nine flavonol glycosides occur in various soybean cultivars with gene t1 resulting in kaempferol and T1 controlling the presence of quercetin plus kaempferol (Buttery and Buzzell, 1973). The sugars of these glycosides have been identified and four flavonol glycoside genes have been studied (Buzzell and Buttery, 1973, and unpublished). A monoglucoside is the basic glycoside; it is present even when the four genes are recessive

Mathematical modelling of tissue-engineering angiogenesis

We present a mathematical model for the vascularisation of a porous scaffold following implantation in vivo. The model is given as a set of coupled non-linear ordinary differential equations (ODEs) which describe the evolution in time of the amounts of the different tissue constituents inside the scaffold. Bifurcation analyses reveal how the extent of scaffold vascularisation changes as a function of the parameter values. For example, it is shown how the loss of seeded cells arising from slow infiltration of vascular tissue can be overcome using a prevascularisation strategy consisting of seeding the scaffold with vascular cells. Using certain assumptions it is shown how the system can be simplified to one which is partially tractable and for which some analysis is given. Limited comparison is also given of the model solutions with experimental data from the chick chorioallantoic membrane (CAM) assay