Определение сакситоксина в воде озера Байкал

Выполнено определение сакситоксина в пробах поверхностной воды, отобранной летом 2018 года в период массового цветения цианобактерий в прибрежной зоне заливов Ая, Тутай (западное побережье Байкала), Мухор (пролив Малое Море), Посольский сор и около села Турка (восточное побережье Байкала). Для определения сакситоксина использована методика определения, основанная на химической модификации сакситоксина 2,4-динитрофенилгидразином с последующей идентификацией гидразона сакситоксина методом жидкостной хроматографии с масс-спектрометрическим детектированием. Для проб с ожидаемой низкой концентрацией сакситоксина предложен усовершенствованный вариант методики. Показано, что содержание сакситоксина в воде во всех исследованных пробах ниже рекомендованной для питьевой воды (< 3 мкг/л)

Prevalence and Severity of Breast Arterial Calcification on Routine Mammography

Aim. To determine the frequency of detection and severity of breast arterial calcification (BAC) among women undergoing mammography on the basis of medical institutions in Moscow.Material and methods. The analysis included 4274 digital mammograms of women aged 40-93 who underwent preventive or diagnostic mammography. Standard full-format digital mammograms were performed in craniocaudal and mediolateral oblique projections. In addition to the standard diagnosis of breast disease, all mammograms were evaluated for the presence of BAC. The severity of BAC was assessed on a 12-point scale: mild 3-4 points, moderate 5-6 points, severe 7-12 points.Results. The average frequency of BAC was 10.1%, in the middle age group 50-59 years – 6.0%. The incidence of BAC increased with age, from 0.4% to 0.6% in women &lt;50 years of age to &gt;50% in women ≥80 years of age. A statistically significant and pronounced correlation was found between the woman's age and the presence of BAC r Pearson =0.769 (p&lt;0.001). There was also a less noticeable but statistically significant correlation between age and severity of BAC r Spearman =0.319 (p&lt;0.001). Regression analysis made it possible to estimate the probability of CAD depending on age. In women &lt;50 years of age, only mild to moderate calcification occurred, while those ≥65 years of age had a significant increase in the incidence of severe CAD.Conclusion. There was an expected increase with age in both prevalence and severity of BAC. Cases of severe BAC in women younger than 65 years of age and any BAC in women younger than 50 years of age are atypical and require clarification of their association with cardiovascular and other diseases

ОПРЕДЕЛЕНИЕ ПОДХОДОВ К ОПЛАТЕ МЕДИЦИНСКОЙ ПОМОЩИ ПО ПРОФИЛЮ «АНЕСТЕЗИОЛОГИЯ-РЕАНИМАТОЛОГИЯ» В УСЛОВИЯХ ПЕРЕХОДА НА НОВУЮ СИСТЕМУ ФИНАНСИРОВАНИЯ ЗДРАВООХРАНЕНИЯ

A new federal model of in-patient medical care funding provides payment for treatment basing on the average costs estimated when evaluating "completed cases" of certain diseases grouped together due to their similarity and types of the provided medical care (CSG system). The lack of differentiation does not allow full reimbursement of expenses of in-patient units occurring during treatment of the severely ill in the intensive care wards. Goal: to estimate risks of financial losses of units providing medical care to the patients with high chances of a complicated course of the disease when medical care is reimbursed within the system described above (CSG system) and to propose improvement of this system. Results. The article presents the results of joint work of the experts from Organizational Economic Committee of the Association of Anesthesiologists and Intensive Care Physicians and workers of St. Petersburg Regional Fund of Mandatory Medical Insurance aimed at the improvement of the medical care funding system based on the so-called clinical statistic groups (CSG). It was suggested splitting up certain clinical statistic groups into subgroups considering the need of patients in the intensive care and its content. The coefficients reflecting the content of costs were calculated for the identified subgroups. The offered approach was piloted during the project in St. Petersburg through estimating costs for a completed case in parallel with estimation as per the existing method of reimbursement related to medical economic standards. The obtained results proved that the offered approach allowed achieving better differentiation due to re-distribution of funds from less severely ill patients who required no treatment in the intensive care departments to the more severely ill. The data were submitted to Center of Expertise and Monitoring of Medical Care Quality in order to prepare suggestions to amend guidelines on medical care reimbursement by the Russian Ministry of Health. Conclusion. The article describes specific practical outcomes – the model was developed to be introduced for reimbursement of the in-patient care as per CSG system within mandatory medical insurance. Новая федеральная модель финансирования медицинской помощи в стационарных условиях предусматривает оплату лечения, исходя из средней стоимости затрат, установленной при оценке «законченных случаев» сгруппированных однотипных заболеваний и видов оказываемой медицинской помощи (КСГ). Отсутствие достаточной дифференциации не позволяет в полной мере компенсировать расходы стационаров, которые возникают при лечении тяжелобольных в отделениях реанимации и интенсивной терапии. Цель: оценить риски финансовых потерь организаций, оказывающих медицинскую помощь пациентам с высокой вероятностью осложненного течения заболевания, при оплате медицинской помощи по системе КСГ и подготовить предложения по совершенствованию данной системы. Результаты. Представлены результаты совместной работы экспертной группы организационно-экономического комитета Ассоциации анестезиологов-реаниматологов и сотрудников Территориального фонда обязательного медицинского страхования (ОМС) Санкт-Петербурга по совершенствованию системы оплаты медицинской помощи в условиях стационара на основе КСГ. Сформулирован подход, предусматривающий разделение отдельных КСГ на подгруппы, с учетом нуждаемости пациентов в «реанимационной» помощи и ее содержания. Для выделенных подгрупп рассчитаны коэффициенты затратоемкости. В рамках пилотного проекта в Санкт-Петербурге проведена апробация предложенной методики расчета законченного случая параллельно с существующим способом оплаты по тарифам, связанным с медико-экономическими стандартами. Результаты показали, что предложенная методика позволяет добиться большей дифференциации за счет перераспределения средств от более «легкой» группы больных, не нуждающихся в лечении в отделениях реанимации и интенсивной терапии, в сторону более «тяжелой». Данные представлены в ФГБУ «Центр экспертизы и контроля качества медицинской помощи» для подготовки предложений по внесению изменений в методические рекомендации по способам оплаты медицинской помощи Минздрава России. Заключение. Получен конкретный практический результат – создана модель для внедрения при оплате стационарной помощи по КСГ в рамках системы ОМС.

Viral ecogenomics across the Porifera

BackgroundViruses directly affect the most important biological processes in the ocean via their regulation of prokaryotic and eukaryotic populations. Marine sponges form stable symbiotic partnerships with a wide diversity of microorganisms and this high symbiont complexity makes them an ideal model for studying viral ecology. Here, we used morphological and molecular approaches to illuminate the diversity and function of viruses inhabiting nine sponge species from the Great Barrier Reef and seven from the Red Sea.ResultsViromic sequencing revealed host-specific and site-specific patterns in the viral assemblages, with all sponge species dominated by the bacteriophage order Caudovirales but also containing variable representation from the nucleocytoplasmic large DNA virus families Mimiviridae, Marseilleviridae, Phycodnaviridae, Ascoviridae, Iridoviridae, Asfarviridae and Poxviridae. Whilst core viral functions related to replication, infection and structure were largely consistent across the sponge viromes, functional profiles varied significantly between species and sites largely due to differential representation of putative auxiliary metabolic genes (AMGs) and accessory genes, including those associated with herbicide resistance, heavy metal resistance and nylon degradation. Furthermore, putative AMGs varied with the composition and abundance of the sponge-associated microbiome. For instance, genes associated with antimicrobial activity were enriched in low microbial abundance sponges, genes associated with nitrogen metabolism were enriched in high microbial abundance sponges and genes related to cellulose biosynthesis were enriched in species that host photosynthetic symbionts.ConclusionsOur results highlight the diverse functional roles that viruses can play in marine sponges and are consistent with our current understanding of sponge ecology. Differential representation of putative viral AMGs and accessory genes across sponge species illustrate the diverse suite of beneficial roles viruses can play in the functional ecology of these complex reef holobionts

Statins and the risk of cataract

Statins are one of the most commonly prescribed classes of drugs for the cardiovascular disease prevention and the treatment of hyperlipidemia. In the literature there is information about the association of statin taking and higher cataract risk. At the same time, there are studies that describe the protective effect of statins on cataracts. Due to the fact that with age the risk cataract increases, as does the likelihood of prescribing statins, the problem of side effects with its use is of particular importance. Despite the conflicting results, many authors agree that there is no convincing reason for statin refuse, since the advantages in cardiovascular risk reducing far outweigh the cataract risk and the possible need for its surgical treatment. The study presents 2 systematic reviews and 3 meta-analyzes about association of statin intake and cataract risk or its surgical treatment, as well as studies carried out over the past 5 years

APPROACHES TO REIMBURSEMENT FOR MEDICAL CARE WITHIN ANESTHESIOLOGY AND INTENSIVE CARE PROFILE DURING TRANSFER TO A NEW SYSTEM OF HEALTH CARE FUNDING

A new federal model of in-patient medical care funding provides payment for treatment basing on the average costs estimated when evaluating "completed cases" of certain diseases grouped together due to their similarity and types of the provided medical care (CSG system). The lack of differentiation does not allow full reimbursement of expenses of in-patient units occurring during treatment of the severely ill in the intensive care wards. Goal: to estimate risks of financial losses of units providing medical care to the patients with high chances of a complicated course of the disease when medical care is reimbursed within the system described above (CSG system) and to propose improvement of this system. Results. The article presents the results of joint work of the experts from Organizational Economic Committee of the Association of Anesthesiologists and Intensive Care Physicians and workers of St. Petersburg Regional Fund of Mandatory Medical Insurance aimed at the improvement of the medical care funding system based on the so-called clinical statistic groups (CSG). It was suggested splitting up certain clinical statistic groups into subgroups considering the need of patients in the intensive care and its content. The coefficients reflecting the content of costs were calculated for the identified subgroups. The offered approach was piloted during the project in St. Petersburg through estimating costs for a completed case in parallel with estimation as per the existing method of reimbursement related to medical economic standards. The obtained results proved that the offered approach allowed achieving better differentiation due to re-distribution of funds from less severely ill patients who required no treatment in the intensive care departments to the more severely ill. The data were submitted to Center of Expertise and Monitoring of Medical Care Quality in order to prepare suggestions to amend guidelines on medical care reimbursement by the Russian Ministry of Health. Conclusion. The article describes specific practical outcomes – the model was developed to be introduced for reimbursement of the in-patient care as per CSG system within mandatory medical insurance

Adherence to Antihypertensive Therapy: A Systematic Review of Russian Prospective Studies from 2000 to 2019

Russia belongs to countries with a high prevalence of arterial hypertension (AH), which is the main cause of premature death in the Russian population. The level of blood pressure (BP) is controlled in less than a third of patients, which may be due to poor adherence to medical recommendations and irregular medication. The manuscript provides a review of studies evaluating the effectiveness of measures to improve adherence to antihypertensive therapy (AHT).Aim. To prepare a systematic review of Russian studies to assess the effectiveness of measures to increase adherence to AHT, to determine/describe the main directions of the intervention and the methodological level.Material and methods. The search for full-text articles on adherence to AHT published in Russian in the period from 2000 to 2019 was carried out in the main Russian and international electronic databases eLIBRARY.ru, Embase, Russian Medicine, MEDLINE. Of the 563 publications found, 20 were included in the review.Results. In 14 studies, adherence was assessed using the 4-item Morisky Medication Adherence Scale (MMAS-4), other studies used bespoke questionnaires or pill counts. Two studies examined factors associated with adherence. The observation period was up 6 weeks to 12 months, the number of participants is 30-2435 people. A higher adherence was noted in women, people over 50 years old, with higher education, working, with concomitant diabetes mellitus and a history of myocardial infarction. Patient education was effective interventions to improve adherence (in particular, in studies, improvement on the MMAS-4 from 1.8 to 3.9 points, p=0.0002 or from 2.80 to 3.79 points, p&lt;0.0001), telephone reminders (p&lt;0.0001), training in self-measurement of blood pressure (p&lt;0.05) and fixed combinations of drugs (p&lt;0.05).Conclusion. The most effective ways to improve adherence are patient education and the use of drugs fixed combinations. In most studies, subjective methods of adherence assessing were used

Antiischemic effects of metoprolol and the risk of carbohydrate metabolism disturbances in angina patients

Aim. To study the association between antiischemic effects (AIE) of metoprolol (MP), glucose tolerance, and insulin sensitivity in patients with stable angina (SA). Material and methods. The study included 28 male patients, aged 46-68 years, with stable effort angina, Functional Class II-III, and positive exercise stress test (EST). The time of the ST segment depression by ≥1 mm defined the threshold exercise stress time. MP in a selected dose was administered twice a day, for one month. Its hemodynamic effects were assessed by the dynamics of heart rate (HR), blood pressure (BP), and double product (DP). Glucose tolerance test (GTT) was performed at baseline (before MP administration) and after one month of MP treatment. Tissue insulin sensitivity and insulin resistance (IR) were assessed by ISI0.120 and HOMA-IR parameters, respectively. Results. AIE was registered in 57% of the patients, while 43% failed to demonstrate it. Both groups did not differ by the extent of MP impact on the levels of HR, BP, and DP. The presence or absence of AIE was linked to selected parameters of glucose metabolism. In patients with AIE, the pre-treatment levels of glucose and insulin 2 hours after glucose load were higher (p=0,028 and 0,043, respectively) and ISI1,120 values lower than in patients without AIE (p=0,023). Among participants with AIE, impaired glucose tolerance (IGT) was observed in 4 at baseline and in 8 one month later; among patients without AIE, IGT was not registered. Conclusion. For the first time, the presence of AIE during MP therapy of SA patients was linked to the decreased insulin sensitivity of peripheral tissues (ISI0.120). Paired EST with a single MP dose at baseline provides an opportunity to identify the patients with a higher risk of metabolic disturbances during the longer-term MP treatment

Carbohydrate metabolism parameters in angina patients treated with metoprolol and its combination with trimetazidine

Aim. To study the effects of a β-adrenoblocker (β-AB) metoprolol (Mp) and its combination with trimetazidine (Tmd) on glucose tolerance and insulin sensitivity in patients with angina pectoris. Material and methods. In total, 28 men aged 46-68 years, with Functional Class (FC) II-III stable angina, positive exercise stress test (EST), and no prior β-AB therapy were examined. Individual Mp doses were selected based on the paired EST results. For one month, the Mp dose of 50 or 100 mg/d was administered twice a day; for the next month, participants received Mp and Tmd (70 mg/d). A standard glucose tolerance test (GTT) was performed at baseline and at the end of one-month periods of Mp or Mp + Tmd treatment. Carbohydrate metabolism disturbances were diagnosed according to the WHO criteria (1999). Insulin resistance (IR) was assessed by HOMA2-IR parameter, and tissue insulin sensitivity by ISI0,120 parameter. Results. After one month of Mp treatment, a decrease in fasting glucose levels was observed (p=0,025). At the same time, the GTT results demonstrated increased glucose levels 2 hours after glucose load, compared to baseline (p=0,049). Tissue insulin sensitivity (ISI0,120) showed some reduction (p=0,14), while the number of patients with impaired glucose tolerance (IGT) increased from 4 to 8 (p=0,006). The levels of fasting and post-load glycemia after one month of the combination therapy with Mp and Tmd were similar to those after the Mp treatment. Insulin levels at 2 hours after glucose load were higher than those observed after the Mp therapy (p=0,045). Compared to baseline, HOMA2-IR values increased, and IDI0,120 values decreased (p=0,036). The IDI0,120 dynamics suggested a reduction in insulin sensitivity for both treatment regimens. IGT was registered in 10 patients. Conclusion. In angina patients, impaired glucose control was observed as early as 1 month after the start of Mp treatment. This early impairment could be diagnosed by GTT. Although the combination therapy with Mp and Tmd did not prevent this impairment, but provided a greater antiischemic effect and, therefore, was clinically appropriat