Developing Identity: Exploring The History Of Indonesian Nationalism

This thesis examines the history of Indonesian nationalism over the course of the twentieth century. In this thesis, I argue that the country’s two main political leaders of the twentieth century, Presidents Sukarno (1945-1967) and Suharto (1967-1998) manipulated nationalist ideology to enhance and extend their executive powers. The thesis begins by looking at the ways that the nationalist movement originated during the final years of the Dutch East Indies colonial period. The first section highlights how the nationalist movement was disunified in its attempts to gain political autonomy from Dutch colonial control. It moves on to talk about the impact of the Japanese occupation period (1942-1945) on the nationalist movement, detailing how Sukarno was able to unify the various nationalist groups by presenting his form of Indonesian ideology, Pancasila. The paper briefly touches on the Indonesian Revolutionary War (1945-1949) before discussing the impact of Sukarno’s gradual move towards communist and anti-Western sentiments. The paper examines several speeches given by Sukarno during this period to emphasize the ways by which he directed national ideology in his favor. The narrative continues to explain the Indonesian public’s backlash against communism, briefly detailing the Communist Massacre of 1965-1966 and explaining how Suharto grabbed power in the ensuing chaos. An analysis of Suharto’s early speeches reveals the ways that Suharto was able to appropriate Sukarno’s Pancasila to fit his own political goals. The thesis moves on to discuss the Pancasila indoctrination programs which Suharto enacts during the late 1970s and into the 1980s and how the Suharto regime became associated with repression and state violence. The thesis concludes by examining the similarities and differences between Sukarno’s and Suharto’s manipulation of ideology to enhance their political agendas

cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG.

Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-total RNA subtractive hybridization strategy has been used that circumvents the problems both of limited availability of bacterial RNA from models of infection and the high rRNA backgrounds in total bacterial RNA. The subtraction products were used to screen a high-density gridded Mycobacterium tuberculosis genomic library. Sequence data were obtained from 19 differential clones, five of which contained overlapping sequences for the gene encoding mycocerosic acid synthase (mas). Mas is an enzyme involved in the synthesis of multi-methylated long-chain fatty acids that are part of phthiocerol dimycocerosate, a major component of the complex mycobacterial cell wall. Northern blotting and primer extension data confirmed up-regulation of mas in intracellular mycobacteria and also revealed a putative extended -10 promoter structure and a long untranslated upstream region 5' of the mas transcripts, containing predicted double-stranded structures. Furthermore, clones containing overlapping sequences for furB, groEL-2, rplE and fadD28 were identified and the up-regulation of these genes was confirmed by Northern blot analysis. The cDNA-RNA subtractive hybridization enrichment and high density gridded library screening, combined with selective extraction of bacterial mRNA represents a valuable approach to the identification of genes expressed during intra-macrophage residence for bacteria such as M. bovis BCG and the pathogenic mycobacterium, M. tuberculosis

Mycobacterium tuberculosis Expresses a Novel Ph-Dependent Divalent Cation Transporter Belonging to the Nramp Family

Mammalian natural resistance–associated macrophage protein (Nramp) homologues are important determinants of susceptibility to infection by diverse intracellular pathogens including mycobacteria. Eukaryotic Nramp homologues transport divalent cations such as Fe2+, Mn2+, Zn2+, and Cu2+. Mycobacterium tuberculosis and Mycobacterium bovis (bacillus Calmette-Guérin [BCG]) also encode an Nramp homologue (Mramp)

Searching in HI for Massive Low Surface Brightness Galaxies: Samples from HyperLeda and the UGC

A search has been made for 21 cm HI line emission in a total of 350 unique galaxies from two samples whose optical properties indicate they may be massive The first consists of 241 low surface brightness (LSB) galaxies of morphological type Sb and later selected from the HyperLeda database and the the second consists of 119 LSB galaxies from the UGC with morphological types Sd-m and later. Of the 350 unique galaxies, 239 were observed at the Nancay Radio Telescope, 161 at the Green Bank Telescope, and 66 at the Arecibo telescope. A total of 295 (84.3%) were detected, of which 253 (72.3%) appear to be uncontaminated by any other galaxies within the telescope beam. Finally, of the total detected, uncontaminated galaxies, at least 31 appear to be massive LSB galaxies, with a total HI mass $\ge$ 10$^{10}$ M$_{sol}$, for H$_0$ = 70 km/s/Mpc. If we expand the definition to also include galaxies with significant total (rather than just gas) mass, i.e., those with inclination-corrected HI line width W$_{50}$,cor > 500 km/s, this bring the total number of massive LSB galaxies to 41. There are no obvious trends between the various measured global galaxy properties, particularly between mean surface brightness and galaxy mass.Comment: 71 pages, including all tables and figures; Accepted by A

4,7-Dichloro­quinoline

The two mol­ecules in the asymmetric unit of the title compound, C9H5Cl2N, are both essentially planar (r.m.s. deviations for all non-H atoms = 0.014 and 0.026 Å). There are no close C—H⋯Cl contacts

Forelimb muscle architecture and myosin isoform composition in the groundhog (Marmota monax)

Scratch-digging mammals are commonly described as having large, powerful forelimb muscles for applying high force to excavate earth, yet studies quantifying the architectural properties of the musculature are largely unavailable. To further test hypotheses about traits that represent specializations for scratch-digging, we quantified muscle architectural properties and myosin expression in the forelimb of the groundhog (Marmota monax), a digger that constructs semi-complex burrows. Architectural properties measured were muscle moment arm, muscle mass (MM), belly length (ML), fascicle length (lF), pennation angle and physiological cross-sectional area (PCSA), and these metrics were used to estimate maximum isometric force, joint torque and power. Myosin heavy chain (MHC) isoform composition was determined in selected forelimb muscles by SDS-PAGE and densitometry analysis. Groundhogs have large limb retractors and elbow extensors that are capable of applying moderately high torque at the shoulder and elbow joints, respectively. Most of these muscles (e.g. latissimus dorsi and pectoralis superficialis) have high lF/ML ratios, indicating substantial shortening ability and moderate power. The unipennate triceps brachii long head has the largest PCSA and is capable of the highest joint torque at both the shoulder and elbow joints. The carpal and digital flexors show greater pennation and shorter fascicle lengths than the limb retractors and elbow extensors, resulting in higher PCSA/MM ratios and force production capacity. Moreover, the digital flexors have the capacity for both appreciable fascicle shortening and force production, indicating high muscle work potential. Overall, the forelimb musculature of the groundhog is capable of relatively low sustained force and power, and these properties are consistent with the findings of a predominant expression of the MHC-2A isoform. Aside from the apparent modifications to the digital flexors, the collective muscle properties observed are consistent with its behavioral classification as a less-specialized burrower and these may be more representative of traits common to numerous rodents with burrowing habits or mammals with some fossorial ability

Ageing, physical function, and the diurnal rhythms of cortisol and dehydroepiandrosterone

The present study examined the relationship between ageing, physical function and the diurnal rhythms of cortisol and dehydroepiandrosterone (DHEA). Participants were 36 community dwelling older adults aged between 65-86 years old. Salivary cortisol and DHEA were measured over the course of one day: immediately upon awakening, 30 min later, and then 3 h, 6 h, 9 h and 12 h post-awakening. Participants completed the Nottingham extended activities of daily living index, the Berg Balance Scale and their handgrip strength was assessed. Older participants had a significantly higher cortisol area under the curve (AUC), lower overall DHEA levels, lower DHEA AUC, a decreased diurnal slope of decline and increased cortisol:DHEA ratio. Lower diurnal cortisol levels were associated with poorer performance on the Berg Balance Scale and lower handgrip strength, and those with a flattened DHEA diurnal profile reported less independence in carrying out daily tasks. These associations withstood adjustment for age. In conclusion, this study suggests an association between cortisol, DHEA, ageing and physical function