230 research outputs found
Prevalence of antibodies to hepatitis B core antigen in blood donors in the middle west region of Brazil
Conventional transarterial chemoembolization versus drug-eluting bead transarterial chemoembolization for the treatment of hepatocellular carcinoma
Genetic variability in the region encompassing reiteration VII of herpes simplex virus type 1, including deletions and multiplications related to recombination between direct repeats
Applications of yeast flocculation in biotechnological processes
A review on the main aspects associated with yeast flocculation and its application
in biotechnological processes is presented. This subject is addressed following three
main aspects – the basics of yeast flocculation, the development of “new” flocculating yeast
strains and bioreactor development. In what concerns the basics of yeast flocculation, the
state of the art on the most relevant aspects of mechanism, physiology and genetics of yeast
flocculation is reported. The construction of flocculating yeast strains includes not only the
recombinant constitutive flocculent brewer’s yeast, but also recombinant flocculent yeast
for lactose metabolisation and ethanol production. Furthermore, recent work on the
heterologous β-galactosidase production using a recombinant flocculent Saccharomyces
cerevisiae is considered. As bioreactors using flocculating yeast cells have particular properties,
mainly associated with a high solid phase hold-up, a section dedicated to its operation is
presented. Aspects such as bioreactor productivity and culture stability as well as bioreactor
hydrodynamics and mass transfer properties of flocculating cell cultures are considered.
Finally, the paper concludes describing some of the applications of high cell density
flocculation bioreactors and discussing potential new uses of these systems.Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI - BD11306/97
A imagina(organiza)ção surrealista: rompendo a gaiola de ferro dos estudos organizacionais
Why has not it worked? An empirical application of the extended Burns and Scapens' framework within the implementation of a controlling department
Performance of two-dimensional Doppler echocardiography for the assessment of infarct size and left ventricular function in rats
Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å
Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 angstrom. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 angstrom. From our electron density map, we located all of the metal atoms of the Mn(4)CaO(5) cluster, together with all of their ligands. We found that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn(4)CaO(5) cluster; some of them may therefore serve as substrates for dioxygen formation. We identified more than 1,300 water molecules in each photosystem II monomer. Some of them formed extensive hydrogen-bonding networks that may serve as channels for protons, water or oxygen molecules. The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail
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