Algae Living in Salamanders, Friend or Foe?

Roughly speaking, our bodies use energy from the sun, but we can\u27t use sunlight directly. Instead, plants and algae collect sunlight and store it as chemical energy through the process of photosynthesis. We can access that fuel directly when we eat plants, or indirectly when we eat other animals that eat plants. However, in some invertebrate animals (those without a backbone) the relationships to algae are more intimate. Tiny single-celled algal symbionts can actually live inside the cells of living corals and small animals like hydra that live in water. The algae live in a safe environment inside animal cells and are provided with building block materials to function. They use sunlight to convert the building block materials into larger molecules to store energy and build cellular structures. At the same time some of that stored solar energy is directly transferred to the host animal, allowing it to live in otherwise nutrient poor environments. Thus the algae and their hosts depend on one another to live and thrive. These mutually beneficial relationships are called photosymbioses. [excerpt

An Application of the Autism Management Platform to Tracking Student Progress in the Special Education Environment

In the age of online courses and digital textbooks, several areas of academia, such as special education, are far behind in the technological revolution. Some teachers use long unstructured digital documents, while others maintain large physical files for students containing every piece of information or coursework they have ever received. Could these extremely unstructured approaches to data collection and aggregation be streamlined with a software platform built specifically for this purpose? Could this platform also be built to accommodate multiple integrations and practical new features? Most importantly, in terms of usability, would this software be enjoyable to use? The Autism Management Platform was initially built for parents of children with Autism to be able to post and view various events and activities throughout their child’s life. The platform now allows for communication on posts between parents, teachers, physicians, and anyone else involved in the child’s life who is authorized to do so. What started as a general platform built for Autism was able to adopt an integration specific to special education. The Autism Management Platform was then further enriched by new features, such as the addition of appointments and visual schedules, proving that it could serve beyond the platform it was intended to be. Through several semester-long user trials in high school special education programs, it was found that real people could use an integration of this platform in an enjoyable and meaningful way

Tablet computers and technological practices within and beyond the laboratory

In this thesis I examine emergent technological practices relating to tablet computers in scientific research laboratories. I ask four main questions: To what extent can tablets be considered scientific instruments? How do tablets help to construct technoscientific imaginaries? What role do tablets play in the construction of technoscientific subjectivities? Can tablets, positioned as popular everyday computing devices, be considered in terms of expertise in the context of laboratory science? To answer these questions, research is presented that examines the situated practices of scientists using tablet computers. I use textual analysis to examine the marketing discourses relating to laboratory-specific tablet apps and how their material structure defines scientific community and communication. Ethnographic research into the way that tablets are being introduced as part of a new teaching laboratory in a large UK university is presented, focusing on how institutional power affects the definition of the tablet. A second ethnographic research case study addresses how two chemists define their own scientific subjectivity by constructing the tablet as a futuristic technology. In a third large ethnographic research case, I consider the way that tablets can be used in practices of inclusion and exclusion from sites of scientific knowledge. I draw on literature from media and cultural studies and science and technology studies, arguing that the two fields intersect in ways that can be productive for research in both. This serves as a contribution to knowledge, demonstrating how research into identity, politics and technologies can benefit from a focus on materiality drawn from the two disciplines. I contribute to knowledge in both fields by developing two key concepts, ‘affordance ambiguity’ and ‘tablet imaginary’. These concepts can be applied in the analysis of uses of technology to better understand, firstly, how technologies are made meaningful for users and, secondly, how this individual meaning-making affects broader cultural trends and understandings of technologies

Doctor of Philosophy

dissertationCardiorespiratory endurance is a major component of health-related fitness testing in physical education. FITNESSGRAM recommends the 1-mile Run/Walk (1-MRW) or the Progressive Aerobic Cardiovascular Endurance Run (PACER) to assess cardiorespiratory endurance by estimating aerobic capacity, or VO2 Peak. No research to date has cross-validated prediction models from both 1-MRW and PACER using current FITNESSGRAM criterion-referenced (CR) standards. Additionally, new prediction models for 1-MRW without a body mass index (BMI) term are needed to attenuate the problems incorporating this index into an aerobic capacity model. The purpose of this dissertation was to cross-validate various prediction models using 1-MRW and PACER and to develop alternative 1-MRW aerobic capacity prediction models for adolescent youth. Participants included 90 students aged 13 to 16 years. Each student completed the 1-MRW and PACER, in addition to a maximal treadmill test to measure VO2 Peak. Multiple correlations among various models with measured VO2 Peak were considered strong (R = 0.74 to 0.78). CR validity, examined using modified kappa (Κq), percentage of agreement (Pa), and phi was considered moderate among all models (Κq = 0.25 to 0.49; Pa = 72% to 79%; phi = 0.38 to 0.65). Two new models were developed from 1-MRW times, one linear and one quadratic model. The linear and quadratic models displayed multiple correlations of R = 0.77 and R = 0.82 with measured VO2 Peak, respectively. CR validity evidence was considered moderate with (Kq = 0.38; Pa = 73%; phi = 0.57) using the linear model and (Kq = 0.34; Pa = 70%; phi = 0.54) using the quadratic model. The accuracy of these models was confirmed using k-fold cross-validation. In conclusion, the prediction models demonstrated strong linear relationships with measured VO2 Peak, acceptable prediction error, and moderate CR agreement with measured VO2 Peak using FITNESSGRAM's CR standards to categorize health groups. The new 1-MRW models displayed good predictive accuracy and moderate CR agreement with measured VO2 Peak without using a BMI predictor. Despite evidence for predictive utility of the new models, they must be externally validated to ensure they can be generalizable to larger populations of students

Transciptome Analysis Illuminates the Nature of the Intracellular Interaction in a Vertebrate-Algal Symbiosis

During embryonic development, cells of the green alga Oophila amblystomatis enter cells of the salamander Ambystoma maculatum forming an endosymbiosis. Here, using de novo dual-RNA seq, we compared the host salamander cells that harbored intracellular algae to those without algae and the algae inside the animal cells to those in the egg capsule. This two-by-two-way analysis revealed that intracellular algae exhibit hallmarks of cellular stress and undergo a striking metabolic shift from oxidative metabolism to fermentation. Culturing experiments with the alga showed that host glutamine may be utilized by the algal endosymbiont as a primary nitrogen source. Transcriptional changes in salamander cells suggest an innate immune response to the alga, with potential attenuation of NF-κB, and metabolic alterations indicative of modulation of insulin sensitivity. In stark contrast to its algal endosymbiont, the salamander cells did not exhibit major stress responses, suggesting that the host cell experience is neutral or beneficial

Junior Recital: Ryan Burns, Horn; Tiffany Ogdon, Piano; April 8, 2022

Kemp Recital HallApril 8, 2023SaturdayNoo

Co-Cultures of Oophila Amblystomatis Between Ambystoma Maculatum and Ambystoma Gracile Hosts Show Host-Symbiont Fidelity

A unique symbiosis occurs between embryos of the spotted salamander (Ambystoma maculatum) and a green alga (Oophila amblystomatis). Unlike most vertebrate host-symbiont relationships, which are ectosymbiotic, A. maculatum exhibits both an ecto- and an endo-symbiosis, where some of the green algal cells living inside egg capsules enter embryonic tissues as well as individual salamander cells. Past research has consistently categorized this symbiosis as a mutualism, making this the first example of a “beneficial” microbe entering vertebrate cells. Another closely related species of salamander, Ambystoma gracile, also harbors beneficial Oophila algae in its egg capsules. However, our sampling within the A. gracile range consistently shows this to be a strict ectosymbiotic interaction—with no sign of tissue or presumably cellular entry. In this study we swapped cultured algae derived from intracapsular fluid of different salamander hosts to test the fidelity of tissue entry in these symbioses. Both A. maculatum and A. gracile embryos were raised in cultures with their own algae or algae cultured from the other host. Under these in vitro culture conditions A. maculatum algae will enter embryonic A. maculatum tissues. Additionally, although at a much lower frequency, A. gracile derived algae will also enter A. maculatum host tissues. However, neither Oophila strain enters A. gracile hosts in these co-culture conditions. These data reveal a potential host-symbiont fidelity that allows the unique endosymbiosis to occur in A. maculatum, but not in A. gracile. However, preliminary trials in our study found that persistent endogenous A. maculatum algae, as opposed to the cultured algae used in subsequent trials, enters host tissues at a higher frequency. An analysis of previously published Oophila transcriptomes revealed dramatic differences in gene expression between cultured and intracapsular Oophila. These include a suite of genes in protein and cell wall synthesis, photosynthesis, central carbon metabolism suggesting the intracapsular algae are assimilating ammonia for nitrogen metabolism and may be undergoing a life-cycle transition. Further refinements of these co-culture conditions could help determine physiological differences between cultured and endogenous algae, as well as rate-limiting cues provided for the alga by the salamander

Senior Recital: Ryan Burns, Horn; Grace Eom, Piano; November 12, 2023

Kemp Recital HallNovember 12, 2023SundayNoo