Observation of cloud-to-ground lightning channels with high-speed video camera

Between May and October 2013 (period of sustained thunderstorm activity in France), several cloud-to-ground lightning flashes have been observed in Paris area with a high-speed video camera (14000 frames per second). The localization and the polarity of the recorded cloud-to-ground flashes have been obtained from the French lightning detection network M{\'e}t{\'e}orage which is equipped with the same low frequency sensors used by the US NLDN. In this paper we focused on 7 events (3 positive cloud-to-ground lightning flashes and 4 negative cloud-to-ground lightning flashes). The propagation velocity of the leaders and its temporal evolution have been estimated; the evolution of branching of the negative leaders have been observed during the propagation of the channel which get connected to ground and initiate the first return stroke. One aim of this preliminary study is to emphasize the differences between the characteristics of the positive and of the negative leaders

Actigraphy in Human African Trypanosomiasis as a Tool for Objective Clinical Evaluation and Monitoring: A Pilot Study

The clinical picture of the parasitic disease human African trypanosomiasis (HAT, also called sleeping sickness) is dominated by sleep alterations. We here used actigraphy to evaluate patients affected by the Gambiense form of HAT. Actigraphy is based on the use of battery-run, wrist-worn devices similar to watches, widely used in middle-high income countries for ambulatory monitoring of sleep disturbances. This pilot study was motivated by the fact that the use of polysomnography, which is the gold standard technology for the evaluation of sleep disorders and has greatly contributed to the objective identification of signs of disease in HAT, faces tangible challenges in resource-limited countries where the disease is endemic. We here show that actigraphy provides objective data on the severity of sleep-wake disturbances that characterize HAT. This technique, which does not disturb the patient's routine activities and can be applied at home, could therefore represent an interesting, non-invasive tool for objective HAT clinical assessment and long-term monitoring under field conditions. The use of this method could provide an adjunct marker of HAT severity and for treatment follow-up, or be evaluated in combination with other disease biomarkers in body fluids that are currently under investigation in many laboratories

Cerebral Changes Occurring in Arginase and Dimethylarginine Dimethylaminohydrolase (DDAH) in a Rat Model of Sleeping Sickness

Involvement of nitric oxide (NO) in the pathophysiology of human African trypanosomiasis (HAT) was analyzed in a HAT animal model (rat infected with Trypanosoma brucei brucei). With this model, it was previously reported that trypanosomes were capable of limiting trypanocidal properties carried by NO by decreasing its blood concentration. It was also observed that brain NO concentration, contrary to blood, increases throughout the infection process. The present approach analyses the brain impairments occurring in the regulations exerted by arginase and N(G), N(G)-dimethylarginine dimethylaminohydrolase (DDAH) on NO Synthases (NOS). In this respect: (i) cerebral enzymatic activities, mRNA and protein expression of arginase and DDAH were determined; (ii) immunohistochemical distribution and morphometric parameters of cells expressing DDAH-1 and DDAH-2 isoforms were examined within the diencephalon; (iii) amino acid profiles relating to NOS/arginase/DDAH pathways were established.Arginase and DDAH activities together with mRNA (RT-PCR) and protein (western-blot) expressions were determined in diencephalic brain structures of healthy or infected rats at various days post-infection (D5, D10, D16, D22). While arginase activity remained constant, that of DDAH increased at D10 (+65%) and D16 (+51%) in agreement with western-blot and amino acids data (liquid chromatography tandem-mass spectrometry). Only DDAH-2 isoform appeared to be up-regulated at the transcriptional level throughout the infection process. Immunohistochemical staining further revealed that DDAH-1 and DDAH-2 are contained within interneurons and neurons, respectively.In the brain of infected animals, the lack of change observed in arginase activity indicates that polyamine production is not enhanced. Increases in DDAH-2 isoform may contribute to the overproduction of NO. These changes are at variance with those reported in the periphery. As a whole, the above processes may ensure additive protection against trypanosome entry into the brain, i.e., maintenance of NO trypanocidal pressure and limitation of polyamine production, necessary for trypanosome growth

The neurology of COVID-19 revisited: A proposal from the environmental neurology specialty group of the world federation of neurology to implement international neurological registries

A comprehensive review of the neurological disorders reported during the current COVID-19 pandemic demonstrates that infection with SARS-CoV-2 affects the central nervous system (CNS), the peripheral nervous system (PNS) and the muscle. CNS manifestations include: headache and decreased responsiveness considered initial indicators of potential neurological involvement; anosmia, hyposmia, hypogeusia, and dysgeusia are frequent early symptoms of coronavirus infection. Respiratory failure, the lethal manifestation of COVID-19, responsible for 264,679 deaths worldwide, is probably neurogenic in origin and may result from the viral invasion of cranial nerve I, progressing into rhinencephalon and brainstem respiratory centers. Cerebrovascular disease, in particular large-vessel ischemic strokes, and less frequently cerebral venous thrombosis, intracerebral hemorrhage and subarachnoid hemorrhage, usually occur as part of a thrombotic state induced by viral attachment to ACE2 receptors in endothelium causing widespread endotheliitis, coagulopathy, arterial and venous thromboses. Acute hemorrhagic necrotizing encephalopathy is associated to the cytokine storm. A frontal hypoperfusion syndrome has been identified. There are isolated reports of seizures, encephalopathy, meningitis, encephalitis, and myelitis. The neurological diseases affecting the PNS and muscle in COVID-19 are less frequent and include Guillain-Barré syndrome; Miller Fisher syndrome; polyneuritis cranialis; and rare instances of viral myopathy with rhabdomyolysis. The main conclusion of this review is the pressing need to define the neurology of COVID-19, its frequency, manifestations, neuropathology and pathogenesis. On behalf of the World Federation of Neurology we invite national and regional neurological associations to create local databases to report cases with neurological manifestations observed during the on-going pandemic. International neuroepidemiological collaboration may help define the natural history of this worldwide problem

Human African Trypanosomiasis: a highly neglected neurological disease

Human African trypanosomiasis (HAT) or sleeping sickness is a severe vector-borne disease, with marked involvement of the peripheral and central nervous system. Still endemic in sub-Saharan Africa, HAT is caused by transmission of subspecies of the protozoan parasites Trypanosoma brucei (T. b.) through bites of tsetse flies (genus Glossina). Foci of HAT are reported mostly in remote, resource-poor settings, and areas of political instability. The disease has a chronic form caused by T. b. gambiense in Western and Central Africa, and an acute form caused by T. b. rhodesiense in Eastern and Southern Africa. Both forms, almost invariably fatal without treatment, evolve from a first, hemolymphatic stage to a second, meningoencephalitic stage due to T. b. brain invasion. Clinical features involve a constellation of sensory, motor and neuropsychiatric signs and symptoms, with a characteristic sleep disorder leading to sleep-wake cycle disorganization and sleep structure alterations. Therapy currently available to cure the second stage of both HAT forms is toxic. Stage biomarkers and safer therapy are urgently needed. Clinical objective evaluation is essential for diagnostic purposes, treatment assessment and patients\u2019 follow-up. The recent decline in the number of reported new HAT cases should not foster further neglect of this highly neglected nervous system infection

Relationships between lightning activity, microphysics and kinematics in thunderclouds: a case study observed by S and C band radars in the South of France (HyMeX SOP area)

International audienceCommunication about Relationships between lightning activity, microphysics and kinematics in thunderclouds: a case study observed by S and C band radars in the South of France (HyMeX SOP area