Tailoring drug release profile of low-molecular-weight hydrogels by supramolecular co-assembly and thiol–ene orthogonal coupling

We describe a general user-friendly platform for fine-tuning the drug release properties of low-molecular-weight hydrogels by a combination of supramolecular co-assembly of complementary molecular structures and controlled photochemical thiol–ene cross-linking. Other critical features such as thermomechanical stability and morphology of the nanostructured hydrogels are also tailored by this approach

Synthesis and studies of molecular tool-box for proteomic

L'objectif majeur de ce travail a été de développer une boite à outil moléculaire permettant la visualisation des protéines dans la cellule et leur identification par spectrométrie de masse. Ces outils ont été conçus pour réagir de façon bioorthogonale avec des sondes chimiques ciblant une classe spécifique de protéine. Le premier objectif a été de développer une approche de marquage fluorescent des protéines en conditions non-covalentes et non-dénaturantes puis de développer un système pour la purification de complexe protéique en conditions non-dénaturantes pour identifier les sous-unités protéiques.The general topic of this work was to develop new chemicals and biochemical tools box allowing proteinsvisualization in cells and identification of proteins by mass spectrometry. These tools were designed to react bioorthogonally with chemicals probes that target a specific class of protein. The first objective was to develop a non-covalent and non-denaturing fluorescent labeling approach of proteins then to develop a system for protein complex purification in non-denaturing conditions in order to identify multisubunit protein complex and functional activity

Synthesis and studies of molecular tool-box for proteomic

L’objectif majeur de ce travail a été de développer une boite à outil moléculaire permettant la visualisation des protéines dans la cellule et leur identification par spectrométrie de masse. Ces outils ont été conçus pour réagir de façon bioorthogonale avec des sondes chimiques ciblant une classe spécifique de protéine. Le premier objectif a été de développer une approche de marquage fluorescent des protéines en conditions non-covalentes et non-dénaturantes puis de développer un système pour la purification de complexe protéique en conditions non-dénaturantes pour identifier les sous-unités protéiques.The general topic of this work was to develop new chemicals and biochemical tools box allowing proteinsvisualization in cells and identification of proteins by mass spectrometry. These tools were designed to react bioorthogonally with chemicals probes that target a specific class of protein. The first objective was to develop a non-covalent and non-denaturing fluorescent labeling approach of proteins then to develop a system for protein complex purification in non-denaturing conditions in order to identify multisubunit protein complex and functional activity

Synthèse et étude d outils moléculaires pour la protéomique

L objectif majeur de ce travail a été de développer une boite à outil moléculaire permettant la visualisation des protéines dans la cellule et leur identification par spectrométrie de masse. Ces outils ont été conçus pour réagir de façon bioorthogonale avec des sondes chimiques ciblant une classe spécifique de protéine. Le premier objectif a été de développer une approche de marquage fluorescent des protéines en conditions non-covalentes et non-dénaturantes puis de développer un système pour la purification de complexe protéique en conditions non-dénaturantes pour identifier les sous-unités protéiques.The general topic of this work was to develop new chemicals and biochemical tools box allowing proteinsvisualization in cells and identification of proteins by mass spectrometry. These tools were designed to react bioorthogonally with chemicals probes that target a specific class of protein. The first objective was to develop a non-covalent and non-denaturing fluorescent labeling approach of proteins then to develop a system for protein complex purification in non-denaturing conditions in order to identify multisubunit protein complex and functional activity.STRASBOURG-Sc. et Techniques (674822102) / SudocSudocFranceF

Engineered Trehalose Permeable to Mammalian Cells

Trehalose is a naturally occurring disaccharide which is associated with extraordinary stress-tolerance capacity in certain species of unicellular and multicellular organisms. In mammalian cells, presence of intra- and extracellular trehalose has been shown to confer improved tolerance against freezing and desiccation. Since mammalian cells do not synthesize nor import trehalose, the development of novel methods for efficient intracellular delivery of trehalose has been an ongoing investigation. Herein, we studied the membrane permeability of engineered lipophilic derivatives of trehalose. Trehalose conjugated with 6 acetyl groups (trehalose hexaacetate or 6-O-Ac-Tre) demonstrated superior permeability in rat hepatocytes compared with regular trehalose, trehalose diacetate (2-O-Ac-Tre) and trehalose tetraacetate (4-O-Ac-Tre). Once in the cell, intracellular esterases hydrolyzed the 6-O-Ac-Tre molecules, releasing free trehalose into the cytoplasm. The total concentration of intracellular trehalose (plus acetylated variants) reached as high as 10 fold the extracellular concentration of 6-O-Ac-Tre, attaining concentrations suitable for applications in biopreservation. To describe this accumulation phenomenon, a diffusion-reaction model was proposed and the permeability and reaction kinetics of 6-O-Ac-Tre were determined by fitting to experimental data. Further studies suggested that the impact of the loading and the presence of intracellular trehalose on cellular viability and function were negligible. Engineering of trehalose chemical structure rather than manipulating the cell, is an innocuous, cell-friendly method for trehalose delivery, with demonstrated potential for trehalose loading in different types of cells and cell lines, and can facilitate the wide-spread application of trehalose as an intracellular protective agent in biopreservation studies

Bioluminescence imaging of small biomolecules

The invention relates to a technique to detect small molecules using Bioluminescence imaging (BLI) to image and quantify non-invasively, in vitro and in vivo,intracellular metabolite fluxes and which can be applied to azido-modified compounds, such as azido-modified biomolecules

A chemical labeling strategy for proteomics under nondenaturing conditions

No abstract availabl

Assessment of hepatocyte functional viability post-incubation with acetylated trehalose.

<p>(A) Overall morphology 1 and 4 days after incubation with 30 mM 6-O-Ac-Tre (scale bar = 20 μm), and (B) a 14-day follow-up of metabolism in rat hepatocytes by measuring daily albumin secretion showed a slow recovery of metabolism in trehalose-loaded cells compared to the control group. (C) The MTT viability assay showed no significant difference in the viabilities of the experimental and the control groups 48 h after trehalose loading with 30 mM 6-O-Ac-Tre (p>0.05). (D) Confocal imaging of mitochondria stained using Mito-Tracker Red 12 h after incubation with 6-O-Ac-Tre showed the presence of mitochondria competent to accumulate dye (dependent on mitochondrial membrane potential, ΔΨ) in both the control (top) and the experiment (bottom) groups. Scale bar = 10 μm (E) JC-1 staining after 6 h incubation with 6-O-Ac-Tre indicates similar mitochondrial ΔΨ in experimental and control groups. A positive control group incubated with the uncoupler CCCP during staining is shown for comparison. Error bars represent SD (n = 3).</p