Investigation of atmospheric insect wing-beat frequencies and iridescence features using a multispectral kHz remote detection system

Quantitative investigation of insect activity in their natural habitat is a challenging task for entomologists. It is difficult to address questions such as flight direction, predation strength, and overall activities using the current techniques such as traps and sweep nets. A multispectral kHz remote detection system using sunlight as an illumination source is presented. We explore the possibilities of remote optical classification of insects based on their wing-beat frequencies and iridescence features. It is shown that the wing-beat frequency of the fast insect events can be resolved by implementing high-sampling frequency. The iridescence features generated from the change of color in two channels (visible and near-infrared) during wing-beat cycle are presented. We show that the shape of the wing-beat trajectory is different for different insects. The flight direction of an atmospheric insect is also determined using a silicon quadrant detector. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE

Observations of movement dynamics of flying insects using high resolution lidar

Insects are fundamental to ecosystem functioning and biodiversity, yet the study of insect movement, dispersal and activity patterns remains a challenge. Here we present results from a novel high resolution laser-radar (lidar) system for quantifying flying insect abundance recorded during one summer night in Sweden. We compare lidar recordings with data from a light trap deployed alongside the lidar. A total of 22808 insect were recorded, and the relative temporal quantities measured matched the quantities recorded with the light trap within a radius of 5 m. Lidar records showed that small insects (wing size 2.5 mm 2 in cross-section) were most abundant near the lidar beam before 22:00 and then moved towards the light trap between 22:00 and 23:30. We could distinguish three insect clusters based on morphology and found that two contained insects predominantly recorded above the field in the evening, whereas the third was formed by insects near the forest at around 21:30. Together our results demonstrate the capability of lidar for distinguishing different types of insect during flight and quantifying their movements

Rare Events in Remote Dark-Field Spectroscopy: An Ecological Case Study of Insects

In this paper, a novel detection scheme for the monitoring of insect ecosystems is presented. Our method is based on the remote acquisition of passive sunlight scattering by two insect species. Procedures to identify rare events in remote dark-field spectroscopy are explained. We further demonstrate how to reduce the spectral representation, and how to discriminate between sexes, using a hierarchical clustering analysis. One-day cycle showing the temporal activities of the two sexes as well as data on activity patterns in relation to temperature and wind is presented. We also give a few examples of the potential use of the technique for studying interactions between sexes on a time scale of milliseconds

Scheimpflug Lidar for combustion diagnostics

A portable Lidar system developed for large-scale (~1-20 m) combustion diagnostics is described and demonstrated. The system is able to perform remote backscattering measurements with range and temporal resolution. The range resolution is obtained by sharply imaging a part of the laser beam onto a CMOS-array or ICCD detector. The large focal depth required to do this is attained by placing the laser beam, the collection optics and the detector in a so-called Scheimpflug configuration. Results from simulations of the range capabilities and range resolution of the system are presented and its temporal resolution is also discussed. Various applications, important for combustion diagnostics, are also demonstrated, including Rayleigh scattering thermometry, aerosol detection and laser-induced fluorescence measurements. These measurements have been carried out using various continuous-wave GaN diode lasers, emitting in the violet-blue (405 – 450 nm) wavelength regime. It is anticipated that Scheimpflug Lidar will provide a useful and versatile diagnostic tool for combustion research, not only for fundamental studies, but in particular for applications at industrial sites

Daily Evolution of the Insect Biomass Spectrum in an Agricultural Landscape Accessed with Lidar

We present measurements of atmospheric insect fauna intercepted by a static lidar transect over arable and pastoral land over one day. We observe nearly a quarter million of events which are calibrated to optical cross section. Biomass spectra are derived from the size distribution and presented against space and time. We discuss detection limits and instrument biasing, and we relate the insect observations to relevant ecological landscape features and land use. Future directions and improvements of the technique are also outlined

Remote nocturnal bird classification by spectroscopy in extended wavelength ranges

We present optical methods at a wide range of wavelengths for remote classification of birds. The proposed methods include eye-safe fluorescence and depolarization lidar techniques, passive scattering spectroscopy, and infrared (IR) spectroscopy. In this paper we refine our previously presented method of remotely classifying birds with the help of laser-induced beta-keratin fluorescence. Phenomena of excitation quenching are studied in the laboratory and are theoretically discussed in detail. It is shown how the ordered microstructures in bird feathers induce structural "colors" in the IR region with wavelengths of around 3-6 mu m. We show that transmittance in this region depends on the angle of incidence of the transmitted light in a species-specific way and that the transmittance exhibits a close correlation to the spatial periodicity in the arrangement of the feather barbules. We present a method by which the microstructure of feathers can be monitored in a remote fashion by utilization of thermal radiation and the wing beating of the bird. (C) 2011 Optical Society of Americ

Dissipative eigenvalue problems for a Sturm-Liouville operator with a singular potential

We present a study of optical signatures of salmon lice and the ability to distinguish them from a reference zooplankton species. This forms the basis for developing an instrument for detecting salmon lice in situ

Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring

Abstract in Undetermined ABSTRACT. Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue optical properties, and ambient light suppression. Laboratory measurements show the system is insensitive to strong ambient light. Validation measurements of tissue phantoms using nonlinear least squares support vector machines (LS-SVM) regression analysis demonstrate an error of <5% for PpIX concentration ranging from 400 to 1000 nM, even in the presence of large variations in phantom optical properties. The mean error is 3% for reduced scattering coefficient and 5% for blood concentration. Diagnostic precision of 100% was obtained by LS-SVM classification for in vivo skin tumors with topically applied 5-aminolevulinic acid during photodynamic therapy. The probe could easily be generalized to other tissue types and fluorophores for therapy guidance and monitoring