Preanalytical processing of archaeological mammal enamel apatite carbonates for stable isotope investigations: a comparative analysis of the effect of acid treatment on samples from Northwest Australia

Stable isotopic analysis of palaeontological and archaeological biogenic apatite carbonates from herbivorous mammalian species represents an important tool for worldwide palaeoecological research. Tooth enamel carbonates are more resistant to taphonomic processes than bone or dentine carbonates but are not invulnerable to diagenesis. As such, they require careful preanalytical processing that considers depositional environment and age. An established part of this process includes a weak acid treatment to remove soluble exogenous carbonates; however, published treatment times for isotopic studies of archaeological tooth enamel are variable and range from 15\ua0min to over 8\ua0hr. This study tests three different pretreatment protocols on modern and Pleistocene age archaeological kangaroo teeth (dating from contemporary to 46,000 bp) to assess the effect of acid treatment time on isotopic integrity. The results indicate that treatment time is a critical parameter for producing consistency across results and shorter pretreatments of 4\ua0hr or less are preferable for removing diagenetic carbonates while minimising alteration of the biological signal

A comparison of two methods of quantifying masticatory pressures developed under dentures with variable occlusal widths

Pressures developed during mastication with removable dental prostheses have beer quantified by two methods. This pilot study (1) compares the peak pressure method of pressure quantification with the integrated curve method of quantification and (2) continues the exploration of the effect of the width of the occlusal table on pressures developed under dentures. A set of specially designed experimental dentures was constructed for one individual. The mandibular experimental denture contained a series of pressure transducers by which masticatory pressures were recorded. These pressures were monitored between the denture base and the residual ridge tissues. Several interchangeable posterior occlusal segments, varying only in the width of the occlusal table, were fabricated for the opposing maxillary denture. Masticatory pressure and total ridge pressure were quantified by the integrated curve method and the peak pressure method. Both methods indicated a direct relationship between masticatory pressures produced and the width of the occlusal table. A similar direct relationship between total ridge pressure and occlusal width was suggested statistically by the integrated curve method, and graphically by both methods.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73368/1/j.1365-2842.1979.tb00407.x.pd

Studies on dental erosion: An in vivo-in vitro model of endogenous dental erosion - its application to testing protection by fluoride gel application

The document attached has been archived with permission from the Australian Dental Association. An external link to the publisher’s copy is included.Background: The objective in this study was to develop an in vivo-in vitro model of endogenous erosion, with a view to exploring the potential for some degree of its control by the use of topical fluoride gel application to teeth. Methods: Six volunteers each wore a small clasp retained palatal acrylic appliance to which six sterilized enamel tiles were bonded. Three tiles on each appliance were coated extra-orally with either 1.23 per cent acidulated phosphate fluoride (APF) or 2.2 per cent sodium fluoride (NaF) neutral gel for four minutes prior to multiple periods of exposure to the simulated gastric acid, cumulating in 16, 36, 80 and 150 minutes of exposure. Impressions of the enamel tiles prior to and following acid exposure permitted dies to be prepared. These were sectioned through the exposed areas and examined under a stereomicroscope to assess maximum depths of erosion. Results: The depth of erosive demineralization of enamel was found to be greatly reduced with increased frequency of APF gel application. The reduction in enamel loss was less following topical application of NaF gel. Conclusions: It was concluded that fluoride gels significantly reduced enamel erosion using this in vivo-in vitro model and therefore, if prescribed appropriately, should help reduce tooth tissue loss from endogenous erosion.L Jones, D Lekkas, D Hunt, J McIntyre and W Rafi

Dentifrices, mouthwashes, and remineralization/caries arrestment strategies

While our knowledge of the dental caries process and its prevention has greatly advanced over the past fifty years, it is fair to state that the management of this disease at the level of the individual patient remains largely empirical. Recommendations for fluoride use by patients at different levels of caries risk are mainly based on the adage that more is better. There is a general understanding that the fluoride compound, concentration, frequency of use, duration of exposure, and method of delivery can influence fluoride efficacy. Two important factors are (1) the initial interaction of relatively high concentrations of fluoride with the tooth surface and plaque during application and (2) the retention of fluoride in oral fluids after application