Padrão geográfico de diversidade genética em populações naturais de Pau-rosa (Aniba rosaeodora), na Amazônia Central

Rosewood (Aniba rosaeodora Ducke, Lauraceae) is an Amazonian evergreen tree and a source of the purest linalool, the main component of its essential oil, which is very valuable in the international perfumery market. After decades of over-exploitation it is currently considered as threatened. We evaluated the genetic diversity and its distribution in four populations in Central Amazonia. Thirty-five reliable RAPD markers were generated, of which 32 were polymorphic (91.4%). Variation was higher within the populations (76.5%; p < 0.0001) and geographic distribution contributed to population differentiation (23.4%; p < 0.0001). The Amazon River had a small influence on gene flow (3.3%; p < 0.0001), but we identified evidence of gene flow across the river. There were significant differences in marker frequencies (p < 0.05), in agreement with the low gene flow (Nm = 2.02). The correlation between genetic distance and gene flow was - 0.95 (p = 0.06) and between geographic distance and gene flow was -0.78 (p = 0.12). There was a geographic cline of variability across an East-West axis, influenced as well by the Amazon River, suggesting the river could be a barrier to gene flow. Although threatened, these Rosewood populations retain high diversity, with the highest levels in the Manaus population, which has been protected for over 42 years in a Reserve.O Pau-rosa (Aniba rosaeodora Ducke, Lauraceae) é uma árvore amazônica fonte do mais puro linalol, o qual é o principal componente do seu óleo essencial e muito valioso no mercado internacional de perfumaria. Após várias décadas de intensa exploração, a espécie foi levada à categoria de ameaçada de extinção. Quatro populações naturais distribuídas na bacia Amazônia Central foram avaliadas quanto ao nível e a distribuição da diversidade genética. Trinta e cinco marcadores RAPD reprodutíveis foram gerados, dos quais 32 foram polimórficos (91,4%). A diversidade foi maior dentro das populações (76,5%; p < 0,0001) e a distribuição geográfica contribuiu para a diferenciação entre as populações (23,4%; p < 0,0001). A AMOVA indicou que pode haver uma influência parcial do Rio Amazonas no fluxo gênico (3,3%; p < 0,0001), mas foram identificadas evidências de fluxo gênico atravessando o rio. Houve diferenças significativas nas freqüências dos marcadores (p < 0,05) e o fluxo gênico estimado foi relativamente baixo (Nm = 2,02). A correlação entre a distância genética e o fluxo gênico foi de - 0,95 (p = 0,06) e para a distância geográfica e o fluxo gênico foi de - 0,78 (p = 0,12). Houve um padrão geográfico de variabilidade ao longo do eixo Leste - Oeste, influenciado também pelo Rio Amazonas, o que sugere que o rio poderia funcionar como uma barreira para o fluxo gênico. Apesar de ameaçadas, estas populações de Pau-rosa possuem alta diversidade, com o maior valor na população de Manaus, que vem sendo protegida por 42 anos em uma reserva

Phylogenomics and the rise of the angiosperms

Angiosperms are the cornerstone of most terrestrial ecosystems and human livelihoods1,2. A robust understanding of angiosperm evolution is required to explain their rise to ecological dominance. So far, the angiosperm tree of life has been determined primarily by means of analyses of the plastid genome3,4. Many studies have drawn on this foundational work, such as classification and first insights into angiosperm diversification since their Mesozoic origins5,6,7. However, the limited and biased sampling of both taxa and genomes undermines confidence in the tree and its implications. Here, we build the tree of life for almost 8,000 (about 60%) angiosperm genera using a standardized set of 353 nuclear genes8. This 15-fold increase in genus-level sampling relative to comparable nuclear studies9 provides a critical test of earlier results and brings notable change to key groups, especially in rosids, while substantiating many previously predicted relationships. Scaling this tree to time using 200 fossils, we discovered that early angiosperm evolution was characterized by high gene tree conflict and explosive diversification, giving rise to more than 80% of extant angiosperm orders. Steady diversification ensued through the remaining Mesozoic Era until rates resurged in the Cenozoic Era, concurrent with decreasing global temperatures and tightly linked with gene tree conflict. Taken together, our extensive sampling combined with advanced phylogenomic methods shows the deep history and full complexity in the evolution of a megadiverse clade

A General Approach To First Order Phase Transitions And The Anomalous Behavior Of Coexisting Phases In The Magnetic Case

First order phase transitions for materials with exotic properties are usually believed to happen at fixed values of the intensive parameters (such as pressure, temperature, etc.) characterizing their properties.. It is also considered that the extensive properties of the phases (such as entropy, volume, etc.) have discontinuities at the transition point, but that for each phase the intensive parameters remain constant during the transition. These features are a hallmark for systems described by two thermodynamic degrees of freedom. In this work it is shown that first order phase transitions must be understood in the broader framework of thermodynamic systems described by three or more degrees of freedom. This means that the transitions occur along intervals of the intensive parameters, that the properties of the phases coexisting during the transition may show peculiar behaviors characteristic of each system, and that a generalized Clausius-Clapeyron equation must be obeyed, These features for the magnetic case are confirmed, and it is shown that experimental caforimetric data agree well with the magnetic Clausius-Clapeyron equation for MnAs. An estimate for the point in the temperature-field plane where the first order magnetic transition turns to a second order one is obtained (the critical parameters) for MnAs and Gd5Ce2Si2 compounds. Anomalous behavior of the volumes of the coexisting phases during the magnetic first order transition is measured, and it is shown that the anomalies for the individual phases are hidden in the behavior of the global properties as the volume. © 2009 WILEY-VCH Verlag GmbH &amp; Co. 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Resposta de cultivares de milho a zinco em solução nutritiva Corn cultivar responses to zinc in nutrient solution

Este trabalho, desenvolvido em solução nutritiva, em casa de vegetação, no Centro Experimental de Campinas, Instituto Agronômico, objetivou definir parâmetros para avaliação e diferenciação de cultivares de milho quanto à exigência em zinco. Desenvolveram-se 24 cultivares durante 30 dias em solução nutritiva completa, com dois níveis de zinco (0,125 e 0,350 mg/L de Zn). O experimento seguiu o delineamento em blocos ao acaso, com parcelas subdivididas, com duas repetições por parcela e quatro plantas de cada cultivar por parcela. Os níveis de zinco ocuparam as parcelas e os cultivares, as subparcelas. Determinaram-se as seguintes variáveis: altura de planta, produção de massa seca da parte aérea, teores e conteúdos de zinco na parte aérea. Observaram-se reduções na produção de massa seca, na altura das plantas e nos teores e conteúdos de zinco, para o nível inferior do micronutriente na solução nutritiva. Os cultivares D-468, ICI-791152 e C-701 mostraram as menores reduções em altura em função do baixo nível de zinco e, C-808, Hatã-2000, OC-5045-6 e BR-201, as maiores respostas ao nível mais alto de zinco. A variação na altura das plantas (sintoma característico da deficiência de zinco) revelou alta correlação (r = 0,86) com a variação no conteúdo de zinco da parte aérea dos diferentes cultivares. A altura das plantas e o conteúdo de zinco constituíram as melhores variáveis para discriminação dos cultivares; entretanto, a técnica em solução nutritiva necessita ser aperfeiçoada para permitir quantificar, com maior precisão, as diferenças detectadas na exigência em zinco.<br>This experiment was conducted in a greenhouse, in nutrient solution, at the Experimental Center of Campinas, Instituto Agronômico, State of São Paulo, Brazil, in order to define variables and parameters to evaluate and differentiate corn cultivars as to zinc requirement. Twenty four corn cultivars were grown in complete nutrient solution with two zinc levels (0.125 and 0.350 mg Zn/ L), during 30 days, in a randomized complete block design, arranged in a split-plot, with two replications and four plants/ cultivar/ plot. Plant height, top dry matter yield, top Zn concentration and content were the traits scored. Low Zn treatment gave rise to reduced dry matter yields and plant heights, as well as to low top Zn concentrations and contents. The cultivars D-468, ICI-791152 and C-701 were the best in the low zinc level. By the other hand, the cultivars C-808, Hatã-2000, OC-5045-6 and BR-201 were more responsive to the high Zn level. High and positive correlation (r = 0,86) was estimated between plant height and top zinc content, which might be useful in future selection schemes. However, more research is needed to quantify, with higher precision, genotype differences in zinc demands