Education begins Egyptian Modernizing

The modern vies with the ancient in Egypt today. Four Egyptian students at Iowa State working for their doctor\u27s degrees in agriculture represent this contrast

The Iowa Homemaker vol.26, no.2

Alma Mater, J. C. Harris, page 2 Travel With Poise, Mary Ann Hakes, page 3 Report From Athens, Joan Kelleher, page 4 Blanche Pederson Interviews an Aussie Bride, Blanche Pederson, page 5 Coeds are Veterans, Too, Mary Margaret Ryan, page 6 Future Home Economics Classroom, Helen Hochriem, page 7 Vicky Grins at the Sun, Breta Soldat, page 9 What’s New in Home Economics, Marjorie Clampitt, page 10 Wardrobe Worries? Here’s What We Wear, Textiles and Clothing Club, page 12 “I Have a Dozen Bosses”, Genevieve Callahan, page 14 Albino Rats Get in on the Ground Floor, Margaret Waterland, page 17 Alums Prove Chemistry can Pay, June Welch, page 18 Education Begins Egyptian Modernizing, Lois Bronson, page 19 Across Alumnae Desks, Mary Neff, page 21 Keeping Up With Today, Joyce Edgar, page 22 How Does Your Garden Grow?, Irene Meyer, page 23 Alums in the News, Goldie Rouse, page 2

VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P2 in yeast and mouse

The signalling lipid PI(3,5)P2 is generated on endosomes and regulates retrograde traffic to the trans-Golgi network. Physiological signals regulate rapid, transient changes in PI(3,5)P2 levels. Mutations that lower PI(3,5)P2 cause neurodegeneration in human patients and mice. The function of Vac14 in the regulation of PI(3,5)P2 was uncharacterized previously. Here, we predict that yeast and mammalian Vac14 are composed entirely of HEAT repeats and demonstrate that Vac14 exerts an effect as a scaffold for the PI(3,5)P2 regulatory complex by direct contact with the known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18. We also report that the mouse mutant ingls (infantile gliosis) results from a missense mutation in Vac14 that prevents the association of Vac14 with Fab1, generating a partial complex. Analysis of ingls and two additional mutants provides insight into the organization of the PI(3,5)P2 regulatory complex and indicates that Vac14 mediates three distinct mechanisms for the rapid interconversion of PI3P and PI(3,5)P2. Moreover, these studies show that the association of Fab1 with the complex is essential for viability in the mouse

Modelling Human Regulatory Variation in Mouse: Finding the Function in Genome-Wide Association Studies and Whole-Genome Sequencing

An increasing body of literature from genome-wide association studies and human whole-genome sequencing highlights the identification of large numbers of candidate regulatory variants of potential therapeutic interest in numerous diseases. Our relatively poor understanding of the functions of non-coding genomic sequence, and the slow and laborious process of experimental validation of the functional significance of human regulatory variants, limits our ability to fully benefit from this information in our efforts to comprehend human disease. Humanized mouse models (HuMMs), in which human genes are introduced into the mouse, suggest an approach to this problem. In the past, HuMMs have been used successfully to study human disease variants; e.g., the complex genetic condition arising from Down syndrome, common monogenic disorders such as Huntington disease and β-thalassemia, and cancer susceptibility genes such as BRCA1. In this commentary, we highlight a novel method for high-throughput single-copy site-specific generation of HuMMs entitled High-throughput Human Genes on the X Chromosome (HuGX). This method can be applied to most human genes for which a bacterial artificial chromosome (BAC) construct can be derived and a mouse-null allele exists. This strategy comprises (1) the use of recombineering technology to create a human variant–harbouring BAC, (2) knock-in of this BAC into the mouse genome using Hprt docking technology, and (3) allele comparison by interspecies complementation. We demonstrate the throughput of the HuGX method by generating a series of seven different alleles for the human NR2E1 gene at Hprt. In future challenges, we consider the current limitations of experimental approaches and call for a concerted effort by the genetics community, for both human and mouse, to solve the challenge of the functional analysis of human regulatory variation

Education begins Egyptian Modernizing

The modern vies with the ancient in Egypt today. Four Egyptian students at Iowa State working for their doctor's degrees in agriculture represent this contrast.</p

The Iowa Homemaker vol.26, no.2

Alma Mater, J. C. Harris, page 2 Travel With Poise, Mary Ann Hakes, page 3 Report From Athens, Joan Kelleher, page 4 Blanche Pederson Interviews an Aussie Bride, Blanche Pederson, page 5 Coeds are Veterans, Too, Mary Margaret Ryan, page 6 Future Home Economics Classroom, Helen Hochriem, page 7 Vicky Grins at the Sun, Breta Soldat, page 9 What’s New in Home Economics, Marjorie Clampitt, page 10 Wardrobe Worries? Here’s What We Wear, Textiles and Clothing Club, page 12 “I Have a Dozen Bosses”, Genevieve Callahan, page 14 Albino Rats Get in on the Ground Floor, Margaret Waterland, page 17 Alums Prove Chemistry can Pay, June Welch, page 18 Education Begins Egyptian Modernizing, Lois Bronson, page 19 Across Alumnae Desks, Mary Neff, page 21 Keeping Up With Today, Joyce Edgar, page 22 How Does Your Garden Grow?, Irene Meyer, page 23 Alums in the News, Goldie Rouse, page 24</p