Antifungal activity of essential oil Hyssopus officinalis L. against micopathogen Mycogone perniciosa (Mang)

The most commonly cultivated mushroom species is the Agaricus bisporus Lange (Imb). One of the major pathogenic diseases of the cultivated mushroom in Serbia is Mycogone perniciosa (Mang). Biological control systems are not much used in mushroom cultivation. Medical and aromatic plants have been placed in the focus of intense studies. Pure culture of the M. perniciosa was isolated from infected A. bisporus. The essential oil of Hyssopus officinalis L. is used as a potential antifungal agent. The most abundant components in oil are isopinocamphone (43.29%), pinocamphone (16.79%) and b-pinene (16.31%). Antifungal activity of Hyssop was investigated by the modified microatmosphere method. The minimal inhibitory quantity was 5 μL/mL and a minimal fungicidal quantity was 15-20 μL/mL. There is no report on the use of Hyssop essential oil in mushroom disease.Agaricus bisporus Lange (Imb) je najčešće komercijalno gajena jestiva gljiva Različiti mikroorganizmi gljive, bakterije i virusi su izazivači bolesti u gajilištima šampinjona. Mycogone perniciosa (Mang) je izazivač bolesti poznate pod nazivom vlažni mehur i najčešći uzročnik gubitaka u gajilištima u Srbiji. Biološka kontrola, koja je uspešno primenjivana na nekim poljoprivrednim kulturama, nije korišćena prilikom uzgoja gljiva. Jedna od mogućnosti je primena biljnih sprejova. Lekovite i aromatične vrste biljaka se intenzivno istražuju kao mogući antifungalni agensi. Uzorci obolelih šampinjona su sakupljani u gajilištima u Srbiji. Kulture M. perniciosa su izolovane sa obolelih plodonosnih tela A. bisporus. Korišćeno je etarsko ulje Hyssopus officinalis. Najzastupljenije komponente ulja su izopinokamfon (43.29%) trans-pinokamfon (16.79%) i b-pinen (16.31%). Antifungalna aktivnost etarskog ulja izopa ispitivana je modifikovanom "mikroatmosfera"- metodom. Minimalna inhibitorna količina je bila 5 μL/mL, a minimalna fungicidna količina 15-20 μL/mL. Velik broj preparata je napravljen i primenjen za kontrolisanje oboljenja pečuraka: fungicidi, primena mikrotalasa ili dejstvo nekih antagonističkih bakterija. Dosad nije bilo saopštenja o primeni etarskog ulja izopa protiv izazivača bolesti gajenih gljiva.nul

Antifungal activity of essential oil Hyssopus officinalis L. against micopathogen Mycogone perniciosa (Mang)

The most commonly cultivated mushroom species is the Agaricus bisporus Lange (Imb). One of the major pathogenic diseases of the cultivated mushroom in Serbia is Mycogone perniciosa (Mang). Biological control systems are not much used in mushroom cultivation. Medical and aromatic plants have been placed in the focus of intense studies. Pure culture of the M. perniciosa was isolated from infected A. bisporus. The essential oil of Hyssopus officinalis L. is used as a potential antifungal agent. The most abundant components in oil are isopinocamphone (43.29%), pinocamphone (16.79%) and b-pinene (16.31%). Antifungal activity of Hyssop was investigated by the modified microatmosphere method. The minimal inhibitory quantity was 5 μL/mL and a minimal fungicidal quantity was 15-20 μL/mL. There is no report on the use of Hyssop essential oil in mushroom disease.Agaricus bisporus Lange (Imb) je najčešće komercijalno gajena jestiva gljiva Različiti mikroorganizmi gljive, bakterije i virusi su izazivači bolesti u gajilištima šampinjona. Mycogone perniciosa (Mang) je izazivač bolesti poznate pod nazivom vlažni mehur i najčešći uzročnik gubitaka u gajilištima u Srbiji. Biološka kontrola, koja je uspešno primenjivana na nekim poljoprivrednim kulturama, nije korišćena prilikom uzgoja gljiva. Jedna od mogućnosti je primena biljnih sprejova. Lekovite i aromatične vrste biljaka se intenzivno istražuju kao mogući antifungalni agensi. Uzorci obolelih šampinjona su sakupljani u gajilištima u Srbiji. Kulture M. perniciosa su izolovane sa obolelih plodonosnih tela A. bisporus. Korišćeno je etarsko ulje Hyssopus officinalis. Najzastupljenije komponente ulja su izopinokamfon (43.29%) trans-pinokamfon (16.79%) i b-pinen (16.31%). Antifungalna aktivnost etarskog ulja izopa ispitivana je modifikovanom "mikroatmosfera"- metodom. Minimalna inhibitorna količina je bila 5 μL/mL, a minimalna fungicidna količina 15-20 μL/mL. Velik broj preparata je napravljen i primenjen za kontrolisanje oboljenja pečuraka: fungicidi, primena mikrotalasa ili dejstvo nekih antagonističkih bakterija. Dosad nije bilo saopštenja o primeni etarskog ulja izopa protiv izazivača bolesti gajenih gljiva.nul

Activity of essential oils of three Micromeria species (Lamiaceae) against micromycetes and bacteria

The chemical composition of essential oils from three Micromeria species: M. dalmatica Benth., M. albanica (Griceb. ex K. Mal) Silic and M. thymifolia (Scop.) Fritsch were investigated by GC and GC-MS and their antibacterial and antifungal activities against seven fungal and six bacterial species were evaluated. Biological assays showed strong fungitoxicity of oils from all three Micromeria spp., particularly M. albanica, against all fungi tested. Essential oils of these species also exerted antibacterial effect against Streptococcus aureus, Escherichia coli, Bacillus subtilis and Micrococcus luteus at low concentrations. Higher concentrations of essential oil of M. albanica and M. dalmatica were active against Gram-negative Pseudomonas aeruginosa, which could be due to the high content of piperitenone oxide. Copyright (C) 2002 John Wiley Sons, Ltd.nul

Activity of essential oils of three Micromeria species (Lamiaceae) against micromycetes and bacteria

The chemical composition of essential oils from three Micromeria species: M. dalmatica Benth., M. albanica (Griceb. ex K. Mal) Silic and M. thymifolia (Scop.) Fritsch were investigated by GC and GC-MS and their antibacterial and antifungal activities against seven fungal and six bacterial species were evaluated. Biological assays showed strong fungitoxicity of oils from all three Micromeria spp., particularly M. albanica, against all fungi tested. Essential oils of these species also exerted antibacterial effect against Streptococcus aureus, Escherichia coli, Bacillus subtilis and Micrococcus luteus at low concentrations. Higher concentrations of essential oil of M. albanica and M. dalmatica were active against Gram-negative Pseudomonas aeruginosa, which could be due to the high content of piperitenone oxide. Copyright (C) 2002 John Wiley Sons, Ltd.nul

Chemical composition and antifungal activity of Salvia desoleana Atzei & Picci essential oil and its major components

The antifungal activity of Salvia desoleana Atzei & Picci (Labiatae) essential oil and its three components was investigated against 17 micromycetes. Among the species tested were food-poisoning fungi and plant, animal and human pathogens. In order to determine minimum inhibitory concentrations (MICs) of the total essential oils and their components, mycelial growth and microdilution tests were used. The essential oil of S. desoleana and linalool possessed strong antifungal activity, 1,8-cineole showed only moderate antifungal potential, while linalyl acetate possessed a lower antifungal potential. Copyright (C) 2009 John Wiley & Sons, Ltd.nul

Chemical composition and antifungal activity of Salvia desoleana Atzei & Picci essential oil and its major components

The antifungal activity of Salvia desoleana Atzei & Picci (Labiatae) essential oil and its three components was investigated against 17 micromycetes. Among the species tested were food-poisoning fungi and plant, animal and human pathogens. In order to determine minimum inhibitory concentrations (MICs) of the total essential oils and their components, mycelial growth and microdilution tests were used. The essential oil of S. desoleana and linalool possessed strong antifungal activity, 1,8-cineole showed only moderate antifungal potential, while linalyl acetate possessed a lower antifungal potential. Copyright (C) 2009 John Wiley & Sons, Ltd.nul

Antifungal activity of essential oil Hyssopus officinalis L. against micopathogen Mycogone perniciosa (Mang)

The most commonly cultivated mushroom species is the Agaricus bisporus Lange (Imb). One of the major pathogenic diseases of the cultivated mushroom in Serbia is Mycogone perniciosa (Mang). Biological control systems are not much used in mushroom cultivation. Medical and aromatic plants have been placed in the focus of intense studies. Pure culture of the M. perniciosa was isolated from infected A. bisporus. The essential oil of Hyssopus officinalis L. is used as a potential antifungal agent. The most abundant components in oil are isopinocamphone (43.29%), pinocamphone (16.79%) and b-pinene (16.31%). Antifungal activity of Hyssop was investigated by the modified microatmosphere method. The minimal inhibitory quantity was 5 μL/mL and a minimal fungicidal quantity was 15-20 μL/mL. There is no report on the use of Hyssop essential oil in mushroom disease

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The in vivo evaluation of the toxicological and antifungal activity of the essential oil of Thymus vulgaris L. and its main component thymol was made on 2-month-old male Wistar rats. We examined the therapeutic potency against experimentally induced dermatomycoses in rats, using the most frequent dermatomycetes, Trichophyton mentagrophytes, T. rubrum, and T. tonsurans. The therapeutic efficacy of a 1% solution of the essential oil of Thymus vulgaris and thymol as well as the commercial preparation bifonazole was evaluated. During the 37-day observation period the oil-treated animals were cured.Ministry of Science of Republic of Serbia [143041, 143049