Rezultate partiale într-o cultură de busuioc în sistem de intercropping

The paper presents the behavior of basil (Ocimum basilicum L.) crop in two intercropping systems with runner bean (Phaseolus coccineus L.) and cherry tomatoes (Licopersicon esculentum L.). The obtained results were evaluated based on morphological and phenological characteristics of the chosen species, revealing the agrophitotechnological character of alimentary basil and its productivity in the two intercropping systems. Due to the decorative valences that basil presents, which were highlighted in the intercropping systems, this species brings unity and harmony to the specific ornamental vegetable garden of our country

Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research

Mass Spectrometry (MS) allows the analysis of proteins and peptides through a variety of methods, such as Electrospray Ionization-Mass Spectrometry (ESI-MS) or Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS). These methods allow identification of the mass of a protein or a peptide as intact molecules or the identification of a protein through peptide-mass fingerprinting generated upon enzymatic digestion. Tandem mass spectrometry (MS/MS) allows the fragmentation of proteins and peptides to determine the amino acid sequence of proteins (top-down and middle-down proteomics) and peptides (bottom-up proteomics). Furthermore, tandem mass spectrometry also allows the identification of post-translational modifications (PTMs) of proteins and peptides. Here, we discuss the application of MS/MS in biomedical research, indicating specific examples for the identification of proteins or peptides and their PTMs as relevant biomarkers for diagnostic and therapy

Approaching Resonant Absorption of Environmental Xenobiotics Harmonic Oscillation by Linear Structures

sustainabilit

Studii de caz referitoare la folosirea design-ului peisager în cultura legumelor

The paper presents an analysis of Romanian household vegetable gardens, based on ornamental criteria and specific vegetable growing technologies characteristic to these plant displays in cultivation. The weak and strong points of the studied vegetable gardens were marked out through a SWOT analysis and based on it solutions were elaborated to improve them on an aesthetic, technologic and workable level in accordance with specialized literature. The obtained results offered unity, balance and an increasing aesthetic value to the plant display, participating in an active mater to the enlargement of their sustainability degree

Evaluating the Thermal Pollution Caused by Wastewaters Discharged from a Chain of Coal-Fired Power Plants along a River

Reliable and safe operation of a coal-fired power plant is strongly linked to freshwater resources, and environmental problems related to water sources and wastewater discharge are challenges for power station operation. In this study, an evaluation on the basis of a wastewater thermal pollution vector is reported for the environmental impact of residual water generated and discharged in the Jiu River during the operation of thermoelectric units of the Rovinari, Turceni and Craiova coal-fired power plants in Romania. Wastewater thermal pollutant vector Plane Projection is applied for assessing the water temperature evolution in the water flow lane created downstream of each power plant wastewater outlet channel. Simulation on the basis of an Electricity of France model, and testing validation of the results for thermoelectric units of 330 MW of these power plants are presented

Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research

Mass Spectrometry (MS) allows the analysis of proteins and peptides through a variety of methods, such as Electrospray Ionization-Mass Spectrometry (ESI-MS) or Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS). These methods allow identification of the mass of a protein or a peptide as intact molecules or the identification of a protein through peptide-mass fingerprinting generated upon enzymatic digestion. Tandem mass spectrometry (MS/MS) allows the fragmentation of proteins and peptides to determine the amino acid sequence of proteins (top-down and middle-down proteomics) and peptides (bottom-up proteomics). Furthermore, tandem mass spectrometry also allows the identification of post-translational modifications (PTMs) of proteins and peptides. Here, we discuss the application of MS/MS in biomedical research, indicating specific examples for the identification of proteins or peptides and their PTMs as relevant biomarkers for diagnostic and therapy

Children’s subjective well-being: A comparability study of 3 psychometric scales in 4 Latin-language speaking countries

En la actualidad, los investigadores así como los políticos están interesados en analizar las diferentes dimensiones que componen el bienestar subjetivo de los niños y los adolescentes, grupos a los cuales se les ha prestado escasa atención si se lo compara con la literatura sobre la población adulta. En esta investigación, se analiza la comparabilidad intercultural de tres escalas de bienestar subjetivo, en estudiantes del primer curso de enseñanza secundaria en Brasil, Chile, España y Rumania. Las escalas multi-ítem muestran buen ajuste con los datos de las muestras agregadas, así como con los respectivos modelos multigrupo con cargas restringidas, avalando la comparabilidad de las correlaciones y regresiones entre las poblaciones. No obstante, los modelos no ajustan con cargas y constantes restringidas, sugiriendo que las medias no son comparables entre países. Así también los resultados muestran puntuaciones en el bienestar subjetivo más altas que las esperadas en poblaciones adultas occidentales. Los resultados del modelo de ecuaciones estructurales que incluye todas las escalas sugieren que las dos escalas por ámbitos participan de un mismo supraconstructo.Currently both, researchers and politicians, are interested in analyzing the many dimensions that are the components of subjective well-being of children and adolescents, since little attention has been paid by the literature to these groups, when compared with adults. In this research we analyze the cross-cultural comparability of three measures of subjective well-being of members of the Freshman Class of High Schools in Brazil, Chile, Spain and Romania. The multi-item scales reveal a good fit with the aggregated samples, as well as with the respective multi-group models with bounded loads, supporting the comparability across correlations and regressions performed on the populations. However, the models do not fit when both loads and constants are bounded, suggesting that the means are not comparable between countries. Also, results reveal that the scores of subjective well-being are higher than those expected from western adult populations.  

Proteomics-Based Identification of Dysregulated Proteins in Breast Cancer

Immunohistochemistry (IHC) is still widely used as a morphology-based assay for in situ analysis of target proteins as specific tumor antigens. However, as a very heterogeneous collection of neoplastic diseases, breast cancer (BC) requires an accurate identification and characterization of larger panels of candidate biomarkers, beyond ER, PR, and HER2 proteins, for diagnosis and personalized treatment, without the limited availability of antibodies that are required to identify specific proteins. Top-down, middle-down, and bottom-up mass spectrometry (MS)-based proteomics approaches complement traditional histopathological tissue analysis to examine expression, modification, and interaction of hundreds to thousands of proteins simultaneously. In this review, we discuss the proteomics-based identification of dysregulated proteins in BC that are essential for the following issues: discovery and validation of new biomarkers by analysis of solid and liquid/non-invasive biopsies, cell lines, organoids and xenograft models; identification of panels of biomarkers for early detection and accurate discrimination between cancer, benign and normal tissues; identification of subtype-specific and stage-specific protein expression profiles in BC grading and measurement of disease progression; characterization of new subtypes of BC; characterization and quantitation of post-translational modifications (PTMs) and aberrant protein–protein interactions (PPI) involved in tumor development; characterization of the global remodeling of BC tissue homeostasis, diagnosis and prognostic information; and deciphering of molecular functions, biological processes and mechanisms through which the dysregulated proteins cause tumor initiation, invasion, and treatment resistance

Dendritic cell immunotherapy versus bevacizumab plus irinotecan in recurrent malignant glioma patients: a survival gain analysis

Stefan-Alexandru Artene,1 Adina Turcu-Stiolica,2 Richard Hartley,1 Marius Eugen Ciurea,3 Oana Daianu,1 Corina Brindusa,1 Oana Alexandru,4 Ligia Gabriela Tataranu,5 Stefana Oana Purcaru,1 Anica Dricu1 1Unit of Biochemistry, 2Department of Biostatistics, 3Department of Plastic and Reconstructive Surgery, 4Department of Neurology, University of Medicine and Pharmacy of Craiova, Craiova, 5Department of Neurosurgery, “Bagdasar–Arseni” Emergency Hospital, Bucharest, Romania Background: The bevacizumab and irinotecan protocol is considered a standard treatment regimen for recurrent malignant glioma. Recent advances in immunotherapy have hinted that vaccination with dendritic cells could become an alternative salvage therapy for the treatment of recurrent malignant glioma.Methods: A search was performed on PubMed, Cochrane Library, Web of Science, ScienceDirect, and Embase in order to identify studies with patients receiving bevacizumab plus irinotecan or dendritic cell therapy for recurrent malignant gliomas. The data obtained from these studies were used to perform a systematic review and survival gain analysis.Results: Fourteen clinical studies with patients receiving either bevacizumab plus irinotecan or dendritic cell vaccination were identified. Seven studies followed patients that received bevacizumab plus irinotecan (302 patients) and seven studies included patients that received dendritic cell immunotherapy (80 patients). For the patients who received bevacizumab plus irinotecan, the mean reported median overall survival was 7.5 (95% confidence interval [CI] 4.84–10.16) months. For the patients who received dendritic cell immunotherapy, the mean reported median overall survival was 17.9 (95% CI 11.34–24.46) months. For irinotecan + bevacizumab group, the mean survival gain was -0.02±2.00, while that for the dendritic cell immunotherapy group was -0.01±4.54.Conclusion: For patients with recurrent malignant gliomas, dendritic cell immunotherapy treatment does not have a significantly different effect when compared with bevacizumab and irinotecan in terms of survival gain (P=0.535) and does not improve weighted survival gain (P=0.620). Keywords: malignant glioma, irinotecan, bevacizumab, dendritic cell, systematic analysi