Contribution à la connaissance des alcaloïdes de Sedum acre

Doctorat en Sciencesinfo:eu-repo/semantics/nonPublishe

Revised sequence of full-length complementary DNA coding for human α1-antitrypsin

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Sedum alkaloids-VI. Resolution enhanced proton NMR and 13C NMR spectroscopic studies on the solution conformation of Sedum acre alkaloids

The preferred solution conformation of 9 Sedum alkaloids and derivatives (sedinine, dihydrosedinine. 8-episedinine, diacetylsedinine, sedinone, 2-episedinone, sedacrine, 2-episedacrine and sedacryptine) was established by high resolution 1H and13C NMR spectroscopy. These compounds may be divided into three classes based on the existence or the absence of an intramolecular hydrogen bond between the nitrogen atom and the hydroxyl group at C8 or at C10. In all of them (except sedacryptine) the N-CH3 group was found to be axially oriented. © 1984.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Highly Effective Detection of Human Papillomavirus 16 and 18 DNA by a Testing Algorithm Combining Broad-Spectrum and Type-Specific PCR

The use of a single broad-spectrum human papillomavirus (HPV) DNA-based PCR test may fail to detect lower concentrations of HPV DNA due to competition between different genotypes in mixed infections. To improve HPV detection by PCR, broad-spectrum and type-specific (TS) PCRs were combined, with a focus on HPV-16 and HPV-18. Cervical and cervicovaginal cell samples were obtained from 1,113 healthy women (age range, 15 to 25 years) participating in an HPV-16/HPV-18 candidate vaccine efficacy trial. These samples were tested by a broad-spectrum SPF(10) PCR-DNA enzyme immunoassay, followed by a primer SPF(10)-based line probe assay (SPF(10) LiPA), and HPV-16- and HPV-18-TS PCRs. The results for the majority of the HPV-16/18 SPF(10) LiPA-positive samples were confirmed by TS-PCR (kappa values, 0.775 for HPV-16 and 0.785 for HPV-18). However, TS PCR revealed additional positive samples among those that contained other HPV genotypes due to competition. Conversely, SPF(10) LiPA identified HPV-16 or -18 in samples that remained negative by TS PCR as a result of sampling variation. Analysis of follow-up samples from more than 1,000 women confirmed that the combination of SPF(10)-LiPA with additional HPV-16- and HPV-18-TS PCR diminishes the rate of false-negative diagnosis. The combination of broad-spectrum and TS PCRs resulted in a novel testing algorithm. This combination of assays is more accurate than either method alone, and the novel algorithm offers a highly accurate and effective method for the analysis of HPV infections

Sedum alkaloids, IV, the X‐ray crystal and molecular structure of sederine hydrochloride and the absolute configuration of sederine

The absolute configuration of the three asymmetric atoms of sederine, a minor alkaloid isolated from Sedum acre, has been established through the use of Horeau's method together with a study by X‐ray diffraction of sederine hydrochloride. Copyright © 1983 Wiley‐VCH Verlag GmbH & Co. KGaA, WeinheimSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Sedum alkaloids. III: A revised structure for sedinine.

Sedinine is assigned the revised structure 2 -which also represents the absolute configuration- on the basis of single-crystal X-ray crystallography. © 1980.SCOPUS: ar.jinfo:eu-repo/semantics/publishe