Purinergic receptor mediated calcium signalling in urothelial cells

Non-neuronal ATP released from the urothelium in response to bladder stretch is a key modulator of bladder mechanosensation. Whilst non-neuronal ATP acts on the underlying bladder afferent nerves to facilitate sensation, there is also the potential for ATP to act in an autocrine manner, modulating urothelial cell function. The aim of this study was to systematically characterise the functional response of primary mouse urothelial cells (PMUCs) to ATP. PMUCs isolated from male mice (14-16 weeks) were used for live-cell fluorescent calcium imaging and qRT-PCR to determine the expression profile of P2X and P2Y receptors. The majority of PMUCs (74-92%) responded to ATP (1 μM-1 mM), as indicted by an increase in intracellular calcium (iCa2+). PMUCs exhibited dose-dependent responses to ATP (10 nM-1 mM) in both calcium containing (2 mM, EC50 = 3.49 ± 0.77 μM) or calcium free (0 mM, EC50 = 9.5 ± 1.5 μM) buffers. However, maximum iCa2+ responses to ATP were significantly attenuated upon repetitive applications in calcium containing but not in calcium free buffer. qRT-PCR revealed expression of P2X1-6, and P2Y1-2, P2Y4, P2Y6, P2Y11-14, but not P2X7 in PMUCs. These findings suggest the major component of ATP induced increases in iCa2+ are mediated via the liberation of calcium from intracellular stores, implicating functional P2Y receptors that are ubiquitously expressed on PMUCs.Russell Chess-Williams, Donna J. Sellers, Stuart M. Brierley, David Grundy, Luke Grund

The monomial representations of the Clifford group

We show that the Clifford group - the normaliser of the Weyl-Heisenberg group - can be represented by monomial phase-permutation matrices if and only if the dimension is a square number. This simplifies expressions for SIC vectors, and has other applications to SICs and to Mutually Unbiased Bases. Exact solutions for SICs in dimension 16 are presented for the first time.Comment: Additional author and exact solutions to the SIC problem in dimension 16 adde

The spiritual revolution and suicidal ideation: an empirical enquiry among 13- to 15-year-old adolescents in England and Wales

The association between conventional religiosity and suicide inhibition has been well explored and documented since the pioneering work of Durkheim. Commentators like Heelas and Woodhead point to ways in which conventional religiosity is giving way in England and Wales to a range of alternative spiritualities, including renewed interest in paranormal phenomena. Taking a sample of 3095 13- to 15-year-old adolescents, the present study examines the association between suicidal ideation and both conventional religiosity and paranormal beliefs, after controlling for individual differences in sex, age and personality (extraversion, neuroticism and psychoticism). The data demonstrate that, while conventional religiosity is slightly associated with lower levels of suicidal ideation, paranormal beliefs are strongly associated with higher levels of suicidal ideation

Tools for Arabic Natural Language Processing: a case study in qalqalah prosody

In this paper, we focus on the prosodic effect of qalqalah or "vibration" applied to a subset of Arabic consonants under certain constraints during correct Qur'anic recitation or taǧwīd, using our Boundary-Annotated Qur’an dataset of 77430 words (Brierley et al 2012; Sawalha et al 2014). These qalqalah events are rule-governed and are signified orthographically in the Arabic script. Hence they can be given abstract definition in the form of regular expressions and thus located and collected automatically. High frequency qalqalah content words are also found to be statistically significant discriminators or keywords when comparing Meccan and Medinan chapters in the Qur'an using a state-of-the-art Visual Analytics toolkit: Semantic Pathways. Thus we hypothesise that qalqalah prosody is one way of highlighting salient items in the text. Finally, we implement Arabic transcription technology (Brierley et al under review; Sawalha et al forthcoming) to create a qalqalah pronunciation guide where each word is transcribed phonetically in IPA and mapped to its chapter-verse ID. This is funded research under the EPSRC "Working Together" theme

Automatically generated, phonemic Arabic-IPA pronunciation tiers for the boundary annotated Qur'an dataset for machine learning (version 2.0)

In this paper, we augment the Boundary Annotated Qur?an dataset published at LREC 2012 (Brierley et al 2012; Sawalha et al 2012a) with automatically generated phonemic transcriptions of Arabic words. We have developed and evaluated a comprehensive grapheme-phoneme mapping from Standard Arabic \ensuremath> IPA (Brierley et al under review), and implemented the mapping in Arabic transcription technology which achieves 100% accuracy as measured against two gold standards: one for Qur?anic or Classical Arabic, and one for Modern Standard Arabic (Sawalha et al [1]). Our mapping algorithm has also been used to generate a pronunciation guide for a subset of Qur?anic words with heightened prosody (Brierley et al 2014). This is funded research under the EPSRC " Working Together" theme

Limited professional guidance and literature are available to guide the safe use of neuromuscular block in infants

Aim Neuromuscular blocking agents (NMBAs) are used in a range of critical illnesses in neonates and infants, despite a lack of guidelines and professional standards. This study reviewed the current evidence base and ascertained UK practice regarding the continuous use of these agents in this age range. Methods We reviewed the literature and carried out a telephone questionnaire of all tertiary units in England and specialist children's hospital neonatal units in the UK. Results No best practice guidelines or general consensus statements were found, and the only randomised trial to feature an NMBA protocol expressed concerns about its use in such young babies. Of the 56 units contacted, 54 (96.4%) shared information. Only three of the 56 (5.4%) used intermittent boluses of NMBAs, 91.1% used NMBA infusions, 11 (19.6%) routinely used regular neuromuscular blocker pause to assess depth, and only one (1.8%) used peripheral nerve stimulation monitoring. All the units carried out clinical assessments, but only one (1.8%) had a written protocol. Conclusion There is a paucity of literature and professional standards to guide the safe use of NMBAs in infants. Of the 54 units who participated in the survey, only one had a protocol for using NMBAs in babies

Health inequalities and health equity challenges for victims of modern slavery

AbstractBackgroundModern slavery is a serious organized crime, with severe consequences for the physical and mental health of victims, and so has public health implications. Anecdotally many victims of sex slavery experience difficulties accessing healthcare. Public Health England recently articulated the importance of health engagement to address modern slavery but little is known about the experiences of the survivors.MethodsWe conducted in depth interviews with Albanian female survivors of sex slavery who all displayed significant and complex health needs. Interviews were conducted between July 2017 and January 2018. Thematic analysis identified four primary themes: (i) barriers to access, (ii) negotiating access, (iii) health needs and care received and (iv) overall experience of primary care.ResultsSurvivors experienced repeated challenges accessing healthcare, for themselves and their children, and initially could not access GP services. When accompanied by an advocate they reported qualitatively and quantitatively improved experiences resulting in improved permeability. Confusion surrounding eligibility criteria and a lack of understanding of modern slavery emerged as the primary barriers, fueling biased adjudications.ConclusionsThe importance of advocates, enabling rights-based approaches, improving understanding about access to health services for vulnerable groups, and a need for education across health service settings are discussed

New class of quantum error-correcting codes for a bosonic mode

We construct a new class of quantum error-correcting codes for a bosonic mode which are advantageous for applications in quantum memories, communication, and scalable computation. These 'binomial quantum codes' are formed from a finite superposition of Fock states weighted with binomial coefficients. The binomial codes can exactly correct errors that are polynomial up to a specific degree in bosonic creation and annihilation operators, including amplitude damping and displacement noise as well as boson addition and dephasing errors. For realistic continuous-time dissipative evolution, the codes can perform approximate quantum error correction to any given order in the timestep between error detection measurements. We present an explicit approximate quantum error recovery operation based on projective measurements and unitary operations. The binomial codes are tailored for detecting boson loss and gain errors by means of measurements of the generalized number parity. We discuss optimization of the binomial codes and demonstrate that by relaxing the parity structure, codes with even lower unrecoverable error rates can be achieved. The binomial codes are related to existing two-mode bosonic codes but offer the advantage of requiring only a single bosonic mode to correct amplitude damping as well as the ability to correct other errors. Our codes are similar in spirit to 'cat codes' based on superpositions of the coherent states, but offer several advantages such as smaller mean number, exact rather than approximate orthonormality of the code words, and an explicit unitary operation for repumping energy into the bosonic mode. The binomial quantum codes are realizable with current superconducting circuit technology and they should prove useful in other quantum technologies, including bosonic quantum memories, photonic quantum communication, and optical-to-microwave up- and down-conversion.Comment: Published versio

Affine Constellations Without Mutually Unbiased Counterparts

It has been conjectured that a complete set of mutually unbiased bases in a space of dimension d exists if and only if there is an affine plane of order d. We introduce affine constellations and compare their existence properties with those of mutually unbiased constellations, mostly in dimension six. The observed discrepancies make a deeper relation between the two existence problems unlikely.Comment: 8 page