The Fetal Allograft Revisited: Does the Study of an Ancient Invertebrate Species Shed Light on the Role of Natural Killer Cells at the Maternal-Fetal Interface?

Human pregnancy poses a fundamental immunological problem because the placenta and fetus are genetically different from the host mother. Classical transplantation theory has not provided a plausible solution to this problem. Study of naturally occurring allogeneic chimeras in the colonial marine invertebrate, Botryllus schlosseri, has yielded fresh insight into the primitive development of allorecognition, especially regarding the role of natural killer (NK) cells. Uterine NK cells have a unique phenotype that appears to parallel aspects of the NK-like cells in the allorecognition system of B. schlosseri. Most notably, both cell types recognize and reject "missing self" and both are involved in the generation of a common vascular system between two individuals. Chimeric combination in B. schlosseri results in vascular fusion between two individual colonies; uterine NK cells appear essential to the establishment of adequate maternal-fetal circulation. Since human uterine NK cells appear to de-emphasize primary immunological function, it is proposed that they may share the same evolutionary roots as the B. schlosseri allorecognition system rather than a primary origin in immunity

Stress response function of a two-dimensional ordered packing of frictional beads

We study the stress profile of an ordered two-dimensional packing of beads in response to the application of a vertical overload localized at its top surface. Disorder is introduced through the Coulombic friction between the grains which gives some indeterminacy and allows the choice of one constrained random number per grain in the calculation of the contact forces. The so-called `multi-agent' technique we use, lets us deal with systems as large as $1000\times1000$ grains. We show that the average response profile has a double peaked structure. At large depth $z$, the position of these peaks grows with $cz$, while their widths scales like $\sqrt{Dz}$. $c$ and $D$ are analogous to `propagation' and `diffusion' coefficients. Their values depend on that of the friction coefficient $\mu$. At small $\mu$, we get $c_0-c \propto \mu$ and $D \propto \mu^\beta$, with $\beta \sim 2.5$, which means that the peaks get closer and wider as the disorder gets larger. This behavior is qualitatively what was predicted in a model where a stochastic relation between the stress components is assumed.Comment: 7 pages, 7 figures, accepted version to Europhys. Let

Discovery of the Optical Counterparts to Four Energetic Fermi Millisecond Pulsars

In the last few years, over 43 millisecond radio pulsars have been discovered by targeted searches of unidentified gamma-ray sources found by the Fermi Gamma-Ray Space Telescope. A large fraction of these millisecond pulsars are in compact binaries with low-mass companions. These systems often show eclipses of the pulsar signal and are commonly known as black widows and redbacks because the pulsar is gradually destroying its companion. In this paper, we report on the optical discovery of four strongly irradiated millisecond pulsar companions. All four sources show modulations of their color and luminosity at the known orbital periods from radio timing. Light curve modelling of our exploratory data shows that the equilibrium temperature reached on the companion's dayside with respect to their nightside is consistent with about 10-30% of the available spin-down energy from the pulsar being reprocessed to increase the companion's dayside temperature. This value compares well with the range observed in other irradiated pulsar binaries and offers insights about the energetics of the pulsar wind and the production of gamma-ray emission. In addition, this provides a simple way of estimating the brightness of irradiated pulsar companions given the pulsar spin-down luminosity. Our analysis also suggests that two of the four new irradiated pulsar companions are only partially filling their Roche lobe. Some of these sources are relatively bright and represent good targets for spectroscopic follow-up. These measurements could enable, among other things, mass determination of the neutron stars in these systems.Comment: 11 pages, 5 tables, 1 figure, 4 online tables. ApJ submitted and referee

The Fetal Allograft Revisited: Does the Study of an Ancient Invertebrate Species Shed Light on the Role of Natural Killer Cells at the Maternal-Fetal Interface?

Human pregnancy poses a fundamental immunological problem because the placenta and fetus are genetically different from the host mother. Classical transplantation theory has not provided a plausible solution to this problem. Study of naturally occurring allogeneic chimeras in the colonial marine invertebrate, Botryllus schlosseri, has yielded fresh insight into the primitive development of allorecognition, especially regarding the role of natural killer (NK) cells. Uterine NK cells have a unique phenotype that appears to parallel aspects of the NK-like cells in the allorecognition system of B. schlosseri. Most notably, both cell types recognize and reject “missing self” and both are involved in the generation of a common vascular system between two individuals. Chimeric combination in B. schlosseri results in vascular fusion between two individual colonies; uterine NK cells appear essential to the establishment of adequate maternal-fetal circulation. Since human uterine NK cells appear to de-emphasize primary immunological function, it is proposed that they may share the same evolutionary roots as the B. schlosseri allorecognition system rather than a primary origin in immunity

Commissioning and operation of the Cherenkov detector for proton Flux Measurement of the UA9 Experiment

The UA9 Experiment at CERN-SPS investigates channeling processes in bent silicon crystals with the aim to manipulate hadron beams. Monitoring and characterization of channeled beams in the high energy accelerators environment ideally requires in-vacuum and radiation hard detectors. For this purpose the Cherenkov detector for proton Flux Measurement (CpFM) was designed and developed. It is based on thin fused silica bars in the beam pipe vacuum which intercept charged particles and generate Cherenkov light. The first version of the CpFM is installed since 2015 in the crystal-assisted collimation setup of the UA9 experiment. In this paper the procedures to make the detector operational and fully integrated in the UA9 setup are described. The most important standard operations of the detector are presented. They have been used to commission and characterize the detector, providing moreover the measurement of the integrated channeled beam profile and several functionality tests as the determination of the crystal bending angle. The calibration has been performed with Lead (Pb) and Xenon (Xe) beams and the results are applied to the flux measurement discussed here in detail.Comment: 25 pages, 14 figure

Development of a sampling ASIC for fast detector signals

International audienceIn the context of the Large Area Picosecond Photodetector (LAPPD) pro ject the motivation to measure time-of-flight at the picosecond reso- lution has pushed towards a faster signal rise-time (below 100 ps) and a higher bandwidth output (higher than 1 GHz) detector, thus, leading to a new signal development and integrity studies of Micro-Channel Plates (MCP) photo-detectors. Similarly, the signal path, is being simulated and characterized, from the anodes to the input of the readout electronics, to minimise losses. Furthermore, to acquire the detector fast pulses a new 10 Gs/s high input bandwidth, 130 nm CMOS sampling chip is being de- veloped

THERMAL DENATURATION OF MONOMERIC AND TRIMERIC PHYCOCYANINS STUDIED BY STATIC AND SPECTROSCOPY POLARIZED TIME-RESOLVED FLUORESCENCE

C-Phycocyanin (PC) and allophycocyanin (APC). as well as the a-subunit of PC. have been isolated from the blue-green alga (cyanobacterium). Spirulina platensis. The effects of partial thermal denaturation of PC and of its state of aggregation have been studied by ps time-resolved, polarized fluorescence spectroscopy. All measurements have been performed under low photon fluxes (< 10’ ’ photonsipulse x cm’) to minimize singlet-singlet annihilation processes. A complex decay is obtained under most conditions, which can be fitted satisfactorily with a bi-exponential (7’ = 70400 ps. T? = 1000-3000 ps) for both the isotropic and the polarized part, but with different intensities and time constants for the two decay curves. The data are interpreted in the frameworkof the model first developed by Teak and Dale (Biochern. J. 116, 161 (1970)], which divides the spectroscopically different chromophores in (predominantly) sensitizing (s) and fluorescing U, ones. If one assumes temperature dependent losses in the energy transfer from the s to the f and between f chromophores. both the biexponential nature of the isotropic fluorescence decay and the polarization data can be rationalized. In the isotropic emission (corresponding to the population of excited states) the short lifetime is related to the s-,f transfer. the longer one to the “free“ decay of the final acceptor(s) (= f). The polarized part is dominated by an extremely short decay time. which is related to s+f transfer, as well as to resonance transfer between the f-chromophores

LOFAR discovery of the fastest-spinning millisecond pulsar in the Galactic field

We report the discovery of PSR J0952$-$0607, a 707-Hz binary millisecond pulsar which is now the fastest-spinning neutron star known in the Galactic field (i.e., outside of a globular cluster). PSR J0952$-$0607 was found using LOFAR at a central observing frequency of 135 MHz, well below the 300 MHz to 3 GHz frequencies typically used in pulsar searches. The discovery is part of an ongoing LOFAR survey targeting unassociated Fermi Large Area Telescope $\gamma$-ray sources. PSR J0952$-$0607 is in a 6.42-hr orbit around a very low-mass companion ($M_\mathrm{c}\gtrsim0.02$ M$_\odot$) and we identify a strongly variable optical source, modulated at the orbital period of the pulsar, as the binary companion. The light curve of the companion varies by 1.6 mag from $r^\prime=22.2$ at maximum to $r^\prime>23.8$, indicating that it is irradiated by the pulsar wind. Swift observations place a 3-$\sigma$ upper limit on the $0.3-10$ keV X-ray luminosity of $L_X < 1.1 \times 10^{31}$ erg s$^{-1}$ (using the 0.97 kpc distance inferred from the dispersion measure). Though no eclipses of the radio pulsar are observed, the properties of the system classify it as a black widow binary. The radio pulsed spectrum of PSR J0952$-$0607, as determined through flux density measurements at 150 and 350 MHz, is extremely steep with $\alpha\sim-3$ (where $S \propto \nu^{\alpha}$). We discuss the growing evidence that the fastest-spinning radio pulsars have exceptionally steep radio spectra, as well as the prospects for finding more sources like PSR J0952$-$0607.Comment: 9 pages, 3 figures, 1 table, published in ApJ letter

Quasi one dimensional 4^4He inside carbon nanotubes

We report results of diffusion Monte Carlo calculations for both $^4$He absorbed in a narrow single walled carbon nanotube (R = 3.42 \AA) and strictly one dimensional $^4$He. Inside the tube, the binding energy of liquid $^4$He is approximately three times larger than on planar graphite. At low linear densities, $^4$He in a nanotube is an experimental realization of a one-dimensional quantum fluid. However, when the density increases the structural and energetic properties of both systems differ. At high density, a quasi-continuous liquid-solid phase transition is observed in both cases.Comment: 11 pages, 3ps figures, to appear in Phys. Rev. B (RC