The Effect of Vaccination on Children's Physical and Cognitive Development in the Philippines

We use data from the Cebu Longitudinal Health and Nutrition Survey in the Philippines to link vaccination in the first two years of life with later physical and cognitive development in children. We use propensity score matching to estimate the causal effect of vaccination on child development. We find no effect of vaccination on later height or weight, but full childhood vaccination for measles, polio, TB, and DPT significantly increases cognitive test scores relative to matched children who received no vaccinations. The size of the effect is large, raising test scores, on average, by about half a standard deviation.vaccination, Phiippines, cognitive development

The relationship between puberty and social emotion processing

The social brain undergoes developmental change during adolescence, and pubertal hormones are hypothesized to contribute to this development. We used fMRI to explore how pubertal indicators (salivary concentrations of testosterone, oestradiol and DHEA; pubertal stage; menarcheal status) relate to brain activity during a social emotion task. Forty-two females aged 11.1 to 13.7 years underwent fMRI scanning while reading scenarios pertaining either to social emotions, which require the representation of another person’s mental states, or to basic emotions, which do not. Pubertal stage and menarcheal status were used to assign girls to early or late puberty groups. Across the entire sample, the contrast between social versus basic emotion resulted in activity within the social brain network, including dorsomedial prefrontal cortex (DMPFC), the posterior superior temporal sulcus, and the anterior temporal cortex (ATC) in both hemispheres. Increased hormone levels (independent of age) were associated with higher left ATC activity during social emotion processing. More advanced age (independent of hormone levels) was associated with lower DMPFC activity during social emotion processing. Our results suggest functionally dissociable effects of pubertal hormones and age on the adolescent social brain

Protocol for optical clearing and imaging of fluorescently labeled ex vivo rat brain slices

Tissue clearing is commonly used for whole-brain imaging but seldom used for brain slices. Here, we present a simple protocol to slice, immunostain, and clear sections of adult rat brains for subsequent high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. We also provide instructions for culturing of rat brain slices free floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change

(RB1)-negative retinal organoids display proliferation of cone photoreceptors and loss of retinal differentiation

Retinoblastoma is a tumor of the eye in children under the age of five caused by biallelic inactivation of the (RB1) tumor suppressor gene in maturing retinal cells. Cancer models are essential for understanding tumor development and in preclinical research. Because of the complex organization of the human retina, such models were challenging to develop for retinoblastoma. Here, we present an organoid model based on differentiation of human embryonic stem cells into neural retina after inactivation of (RB1) by CRISPR/Cas9 mutagenesis. Wildtype and (RB1) heterozygous mutant retinal organoids were indistinguishable with respect to morphology, temporal development of retinal cell types and global mRNA expression. However, loss of pRB resulted in spatially disorganized organoids and aberrant differentiation, indicated by disintegration of organoids beyond day 130 of differentiation and depletion of most retinal cell types. Only cone photoreceptors were abundant and continued to proliferate, supporting these as candidate cells-of-origin for retinoblastoma. Transcriptome analysis of (RB1) knockout organoids and primary retinoblastoma revealed gain of a retinoblastoma expression signature in the organoids, characterized by upregulation of (RBL1) (p107), (MDM2), (DEK), (SYK) and (HELLS). In addition, genes related to immune response and extracellular matrix were specifically upregulated in (RB1)-negative organoids. In vitro retinal organoids therefore display some features associated with retinoblastoma and, so far, represent the only valid human cancer model for the development of this disease

A Resource Guide to Support Minority Students at the Elementary Grade in the Kent School District with Learning Difficulties in the Area of Reading

A literature review was conducted to illustrate that there is an achievement gap in education between minority and white students. Probable causes contributing to the achievement gap and possible solutions to the gap were studied. The knowledge that high standards, a challenging curriculum and good teachers (Haycock, 2001), increases student achievement of minority students, inspired a project geared toward providing teachers with strategies to support minority students with learning difficulties in the area of reading. A model reading strategy guide featuring reading strategies to meet the needs of diverse learners was developed for presentation to Springbrook Elementary School. The guide listed various methods teachers can utilize in order to better meet the needs of minority students in the classroom. The results ofthis guide actually point to the fact that good teaching is an effective way not only to meet the needs of minority students, but also meet the needs of all students

The effect of vaccination on children's physical and cognitive development in the Philippines

We use data from the Cebu Longitudinal Health and Nutrition Survey (CLHNS) in the Philippines to link vaccination in the first 2 years of life with later physical and cognitive development in children. We use propensity score matching to estimate the causal effect of vaccination on child development. We find no effect of vaccination on later height or weight, but full childhood vaccination for measles, polio, Tuberculosis (TB), Diphtheria, Pertussis and Tetanus (DPT) significantly increases cognitive test scores relative to matched children who received no vaccinations. The size of the effect is large, raising test scores, on average, by about half an SD

Rankings and higher education: reframing relationships within and between states

Global university rankings are playing an increasing role in shaping higher education’s purpose, argues Professor Ellen Hazelkorn from Dublin Institute of Technology in a paper written for the 2017 Burton R Clark annual lecture on higher education. In the paper, she argues that despite ongoing criticism of university rankings, and the appropriateness or otherwise of the methodology, they are now widely perceived and used as the international measure of quality. Surveys that she conducted in 2006 and again in 2014 show that universities are using rankings to inform strategic decisions and shape priorities. Being in the ‘top 100’ is widely formulated as a national or institutional strategy. Professor Hazelkorn contends that the dominance of rankings has arisen as a result of the changing relationship between higher education and the state. She identifies this change as resulting from: 1) growing calls for accountability, and 2) the increasingly geopolitical responsibilities of universities. She highlights the fact that in recent years there has been growing pressure on universities to justify the public interests they serve. As a result, quality has become a contested concept, transformed from being institutionally led to driven and regulated by the state. Globalisation and the massification of higher education have also led to a growing demand for internationally comparative data. University priorities are being set by governments through national strategies or performance agreements. Whereas historically the state provided for the needs of universities, today the university provides for the needs of the state. She argues that the popularity of rankings also reflects higher education’s central position in geopolitical relations. As nations compete based on their knowledge and innovation systems, higher education has become an important part of the global economic architecture. Universities face competing demands from local, national and global actors. As a consequence, the pursuit of ‘world-class’ status has become a shared strategy of transnationalising elites. Professor Hazelkorn warns that one of the dangers of global rankings is that many universities have become civically disengaged. By measuring the achievements of individual universities rather than the system or society collectively, global rankings promote world-class universities rather than world-class systems. Professor Hazelkorn concludes that universities must urgently rethink and reshape relations with their publics and the state, and work towards bridging the gap between local, national and global

Sero-Epidemiological Study of Bordetella pertussis Infection in the Italian General Population

A multicenter study was conducted to estimate the prevalence of pertussis IgG antibodies (anti-PTx) in the Italian population. Serum samples (4154) collected in the years 2019-2020 from subjects aged 6 to 90 years were tested. The anti-PTx IgG levels were determined by ELISA test. The limit of detection was 5 IU/mL (International Units per milliliter); values >= 40 IU/mL and >= 100 IU/mL indicate an infection that has occurred in recent years and a recent infection (occurred during the last year), respectively. The mean concentration of anti-PTx IgG antibodies in the tested samples was 13 IU/mL; 1.0% of subjects had a titer >= 100 IU/mL, 5.3% a titer between 40 and 100 IU/mL, and 38.9% a titer < 5 IU/mL. The mean antibody concentration was significantly higher in males than in females. The age group 25-39 years had the lowest percentage of negative subjects (36.9%) and the highest prevalence of subjects with antibody titers >= 100 IU/mL (1.3%). In the age group >= 65 years, the prevalence of subjects with titers between 40 and 100 IU/mL (6.7%) and the percentage of negative subjects (44.8%) was higher than in the other age groups. The results highlight the possible role of adolescents and adults in the transmission of B. pertussis