38 research outputs found
EXOPEPTIDASE CATALYZED SITE-SPECIFIC BONDING OF SUPPORTS, LABELS AND BIOACTIVE AGENTS TO PROTEINS
Get PDFAn auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein
The use of a glutamic acid specific endopeptidases in peptide synthesis
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Carboxypeptidase S-1 from Penicillium janthinellum: Enzymatic properties in hydrolysis and aminolysis reactions
No full textDetermination of C-terminal sequences by digestion with serine carboxypeptidases: The influence of enzyme specificity
No full textPeptide Substrates Dissolved in Dimethylformamide May Be Modified at the ϵ-Amino Group of Lysyl Residues, Causing Erroneous Kinetic Characterization of Proteolytic Enzymes
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