3 research outputs found
Ion microprobe age and geochemistry of southern Appalachian basement, with implications for Proterozoic and Palaeozoic reconstructions
No full textCytokine mRNA repertoire of peripheral blood mononuclear cells in Takayasu's arteritis
Get PDFWe have investigated constitutive and phytohaemagglutinin (PHA) + phorbol 12-myristate 13-acetate (PMA)-induced gene expression of tumour necrosis factor (TNF)-伪, interferon (IFN)-纬, interleukin (IL)-2, IL-3, IL-4, IL-10, IL-12 and granulocyte macrophage colony-stimulating factor (GM-CSF) in peripheral blood mononuclear cells (PBMCs) of 10 patients with Takayasu's arteritis (TA) and 10 healthy controls by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). The constitutive mRNA expression of TNF-伪 (69路0 卤 4路0% versus 27路5 卤 18路0%; P = 0路001) and IL-4 (60路0 卤 10路0% versus 0%; P = 0路001) was significantly higher in patients than controls; that of IL-3 was comparable in both groups (38路0 卤 6路0% versus 32路0 卤 5路0%; P = 0路651) while no constitutive mRNA expression was observed for the other cytokines studied. The stimulated PBMCs of patients, as compared with the controls, had higher mRNA gene expression of TNF-伪 (127路0 卤 16路0% versus 54路0 卤 6路0%; P = 0路001), IFN-纬 (93路0 卤 13路0% versus 57路0 卤 5路0%; P = 0路032), IL-2 (109路0 卤 13路0% versus 68路0 卤 6路0%; P = 0路015), IL-3 (60路0 卤 8路0% versus 21路2 卤 3路0%; P = 0路045) and IL-4 (68路0 卤 7路0% versus 27路0 卤 7路2%; P = 0路01) The mRNA expression of IL-10 was lower in patients than controls (35路0 卤 8路0% versus 75路0 卤 12路0%; P = 0路022). The GM-CSF mRNA was similar (102路0 卤 6路0% versus 89路0 卤 5路0%; P = 0路475) in both groups. Stimulation of cells with PHA + PMA showed no IL-12 expression but stimulation with lipopolysaccharide induced higher IL-12 mRNA in patients than controls (83路0 卤 14路0% versus 33路0 卤 4路0%; P = 0路005). Our data suggest that an inflammatory cytokine signature exists in TA with a key role for TNF-伪, IL-4, IL-10 and IL-12 in different pathological processes of the disease
