Artificial Intelligence

Contains research objectives and reports on five research projects.Computation Center, M.I.T

Software Model Checking with Explicit Scheduler and Symbolic Threads

In many practical application domains, the software is organized into a set of threads, whose activation is exclusive and controlled by a cooperative scheduling policy: threads execute, without any interruption, until they either terminate or yield the control explicitly to the scheduler. The formal verification of such software poses significant challenges. On the one side, each thread may have infinite state space, and might call for abstraction. On the other side, the scheduling policy is often important for correctness, and an approach based on abstracting the scheduler may result in loss of precision and false positives. Unfortunately, the translation of the problem into a purely sequential software model checking problem turns out to be highly inefficient for the available technologies. We propose a software model checking technique that exploits the intrinsic structure of these programs. Each thread is translated into a separate sequential program and explored symbolically with lazy abstraction, while the overall verification is orchestrated by the direct execution of the scheduler. The approach is optimized by filtering the exploration of the scheduler with the integration of partial-order reduction. The technique, called ESST (Explicit Scheduler, Symbolic Threads) has been implemented and experimentally evaluated on a significant set of benchmarks. The results demonstrate that ESST technique is way more effective than software model checking applied to the sequentialized programs, and that partial-order reduction can lead to further performance improvements.Comment: 40 pages, 10 figures, accepted for publication in journal of logical methods in computer scienc

A comparative modeling of supernova 1993J

The light curve of Supernova (SN) 1993J is calculated using two approaches to radiation transport as exemplified by the two computer codes, STELLA and EDDINGTON. Particular attention is paid to shock breakout and the photometry in the U, B, and V bands during the first 120 days. The hydrodynamical model, the explosion of a 13 Msun star which had lost most of its hydrogenic envelope to a companion, is the same in each calculation. The comparison elucidates differences between the approaches and also serves to validate the results of both. STELLA includes implicit hydrodynamics and is able to model supernova evolution at early times, before the expansion is homologous. STELLA also employs multi-group photonics and is able to follow the radiation as it decouples from the matter. EDDINGTON uses a different algorithm for integrating the transport equation, assumes homologous expansion, and uses a finer frequency resolution. Good agreement is achieved between the two codes only when compatible physical assumptions are made about the opacity. A new result for SN 1993J is a prediction of the continuum spectrum near the shock breakout (calculated by STELLA) which is superior to the results of other standard single energy group hydrocodes such as VISPHOT or TITAN. Based on the results of our independent codes, we discuss the uncertainties involved in the current time dependent models of supernova light curves.Comment: 43 pages with 22 eps figures, aaspp4.sty + epsf.sty, Accepted by ApJ, to appear in March 20, 1998 issue, Vol. 49

Variable Reuse for Efficient Image Computation

Image computation, that is, computing the set of states reachable from a given set in one step, is a crucial component in typical tools for BDD-based symbolic reachability analysis. It has been shown that the size of the intermediate BDDs during image computation can be dramatically reduced via conjunctive partitioning of the transition relation and ordering the conjuncts for facilitating early quantification. In this paper, we propose to enhance the effectiveness of these techniques by reusing the quantified variables. Given an ordered set of conjuncts, if the last conjunct that uses a variable u appears before the first conjunct that uses another variable v, then v can be renamed to u, assuming u will be quantified immediately after its last use. In general, multiple variables can share the same identifier so the BDD nodes that are inactive but not garbage collected may be activated. We give a polynomial-time algorithm for generating the optimum number of variables that are required for image computation and show how to modify the image computation accounting for variable reuse. The savings for image computation are demonstrated on ISCAS\u2789 and Texas\u2797 benchmark models

The alpha 7 nicotinic receptor agonist PHA-543613 hydrochloride inhibits <i>Porphyromonas gingivalis</i>-induced expression of interleukin-8 by oral keratinocytes

Objective: The alpha 7 nicotinic receptor (α7nAChR) is expressed by oral keratinocytes. α7nAChR activation mediates anti-inflammatory responses. The objective of this study was to determine if α7nAChR activation inhibited pathogen-induced interleukin-8 (IL-8) expression by oral keratinocytes.&lt;p&gt;&lt;/p&gt; Materials and methods: Periodontal tissue expression of α7nAChR was determined by real-time PCR. OKF6/TERT-2 oral keratinocytes were exposed to &lt;i&gt;Porphyromonas gingivalis&lt;/i&gt; in the presence and absence of a α7nAChR agonist (PHA-543613 hydrochloride) alone or after pre-exposure to a specific α7nAChR antagonist (α-bungarotoxin). Interleukin-8 (IL-8) expression was measured by ELISA and real-time PCR. Phosphorylation of the NF-κB p65 subunit was determined using an NF-κB p65 profiler assay and STAT-3 activation by STAT-3 in-cell ELISA. The release of ACh from oral keratinocytes in response to &lt;i&gt;P. gingivalis&lt;/i&gt; lipopolysaccharide was determined using a GeneBLAzer M3 CHO-K1-blacell reporter assay.&lt;p&gt;&lt;/p&gt; Results: Expression of α7nAChR mRNA was elevated in diseased periodontal tissue. PHA-543613 hydrochloride inhibited &lt;i&gt;P. Gingivalis&lt;/i&gt;-induced expression of IL-8 at the transcriptional level. This effect was abolished when cells were pre-exposed to a specific α7nAChR antagonist, α-bungarotoxin. PHA-543613 hydrochloride downregulated NF-κB signalling through reduced phosphorylation of the NF-κB p65-subunit. In addition, PHA-543613 hydrochloride promoted STAT-3 signalling by maintenance of phosphorylation. Furthermore, oral keratinocytes upregulated ACh release in response to &lt;i&gt;P. Gingivalis&lt;/i&gt; lipopolysaccharide.&lt;p&gt;&lt;/p&gt; Conclusion: These data suggest that α7nAChR plays a role in regulating the innate immune responses of oral keratinocytes.&lt;p&gt;&lt;/p&gt

Colloidal suspensions in modulated light fields

Periodically-modulated potentials in the form of light fields have previously been applied to induce reversible phase transitions in dilute colloidal systems with long-range interactions. Here we investigate whether similar transitions can be induced in very dense systems, where inter-particle contacts are important. Using microscopy we show that particles in such systems are indeed strongly affected by modulated potentials. We discuss technical aspects relevant to generating the light-induced potentials and to imaging simultaneously the particles. We also consider what happens when the particle size is comparable with the modulation wavelength. The effects of selected modulation wavelengths as well as pure radiation pressure are illustrated.Comment: Accepted by J. Phys.: Condens. Matter, CODEF II Special Issue. 23 pages, 12 figure

Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus

The aim of this study was to determine the susceptibility of murine embryonic stem (mESCs) to mouse hepatitis virus (MHV-A59) and mouse minute virus (MMVp) and the effect of these viruses on germline transmission (GLT) and the serological status of recipients and pups. When recipients received 10 blastocysts, each injected with 100 TCID50 MHV-A59, three out of five recipients and four out of 14 pups from three litters became seropositive. When blastocysts were injected with 10−5 TCID50 MMVp, all four recipients and 14 pups from four litters remained seronegative. The mESCs replicated MHV-A59 but not MMVp, MHV-A59 being cytolytic for mESCs. Exposure of mESCs to the viruses over four to five passages but not for 6 h affected GLT. Recipients were seropositive for MHV-A59 but not for MMVp when mESCs were cultured with the virus over four or five passages. The data show that GLT is affected by virus-contaminated mESCs

SAT-Based Combinational and Sequential Dependency Computation

We present an algorithm for computing both functional dependency and unateness of combinational and sequential Boolean func- tions represented as logic networks. The algorithm uses SAT-based tech- niques from Combinational Equivalence Checking (CEC) and Automatic Test Pattern Generation (ATPG) to compute the dependency matrix of multi-output Boolean functions. Additionally, the classical dependency definitions are extended to sequential functions and a fast approximation is presented to efficiently yield a sequential dependency matrix. Exten- sive experiments show the applicability of the methods and the improved robustness compared to existing approaches