El combatir el terror de estado y la evolución hacía un estado: El movimiento de los Derechos Humanos de Argentina, desde la movilización a la institucionalización

The past decade has seen a seismic shift in Argentine human rights activists’ ability to accomplish long-held goals of bringing perpetrators to justice and preserving the memory of victims of the Civil-State dictatorship of the 1970s and 1980s. Theories of courageous resistance to injustice posit that all struggles for justice bring changes to the context, networks and people, which can make future pro-social advocacy more likely. Using a variety of evidence, we assess the impact of nearly 40 years of sustained activism. We argue that there are resulting shifts in the realms of 1) context (newly created and modified legal and state institutions, cultural changes and modification of the physical landscape), 2) relationships between activists, society and the Argentine state, and 3) socialization factors that affect attitudes and behaviors of Argentines (the ways that the era is referenced, taught and memorialized). We contend that each of these enduring legacies of activism make future human rights atrocities in Argentina highly unlikely. Further, we argue that because of these enormous gains, segments of the movement struggle to define themselves relative to the state, trying to avoid being captured by governmental interests and to maintain sufficient independence to effectively pressure the state.La década pasada ha visto un cambio sísmico en la habilidad de activistas argentinos por los derechos humanos de realizar metas largamente sostenidas, como las de llevar ante la justicia a los perpetradores y preservar la memoria de las víctimas de dictadura civil-estatal de los años setenta y ochenta. Las teorías de courageous resistance a la injusticia postulan que toda lucha por justicia produce cambios en el contexto, las redes y las personas, los cuales pueden mejorar la probabilidad de incidencia política prosocial para el futuro. En base a una variedad de evidencia, evaluamos el impacto de casi 40 años de activismo sostenido. Argumentamos que hay cambios resultantes en las esferas de 1) el contexto (instituciones legales y estatales nuevamente creadas y modificadas, cambios culturales y modificaciones al paisaje físico), 2) las relaciones entre activistas, la sociedad y el Estado argentino y 3) factores en la socialización que afectan las actitudes y el comportamiento de argentinos (cambios en las formas de referenciar, enseñar y conmemorar la época). Sostenemos que cada uno de estos importantes y duraderos legados de activismo hace que futuras atrocidades de derechos humanos en la Argentina sean altamente improbables. Además, argumentamos que, a causa de estos logros, sectores del movimiento se definen difícilmente con respecto al Estado, intentando evitar la captura por los intereses gubernamentales y mantener una independencia suficiente para presionar eficazmente al Estado

Structural Basis of BRCC36 Function in DNA Repair and Immune Regulation

In mammals, ∼100 deubiquitinases act on ∼20,000 intracellular ubiquitination sites. Deubiquitinases are commonly regarded as constitutively active, with limited regulatory and targeting capacity. The BRCA1-A and BRISC complexes serve in DNA double-strand break repair and immune signaling and contain the lysine-63 linkage-specific BRCC36 subunit that is functionalized by scaffold subunits ABRAXAS and ABRO1, respectively. The molecular basis underlying BRCA1-A and BRISC function is currently unknown. Here we show that in the BRCA1-A complex structure, ABRAXAS integrates the DNA repair protein RAP80 and provides a high-affinity binding site that sequesters the tumor suppressor BRCA1 away from the break site. In the BRISC structure, ABRO1 binds SHMT2α, a metabolic enzyme enabling cancer growth in hypoxic environments, which we find prevents BRCC36 from binding and cleaving ubiquitin chains. Our work explains modularity in the BRCC36 DUB family, with different adaptor subunits conferring diversified targeting and regulatory functions.ISSN:1097-2765ISSN:1097-416

Fighting State Terror and Becoming the State: Argentina’s Human Rights Movement from Mass Mobilization to Institutionalization

The past decade has seen a seismic shift in Argentine human rights activists’ ability to accomplish long-held goals of bringing perpetrators to justice and preserving the memory of victims of the Civil-State dictatorship of the 1970s and 1980s. Theories of courageous resistance to injustice posit that all struggles for justice bring changes to the context, networks and people, which can make future pro-social advocacy more likely. Using a variety of evidence, we assess the impact of nearly 40 years of sustained activism. We argue that there are resulting shifts in the realms of 1) context (newly created and modified legal and state institutions, cultural changes and modification of the physical landscape), 2) relationships between activists, society and the Argentine state, and 3) socialization factors that affect attitudes and behaviors of Argentines (the ways that the era is referenced, taught and memorialized). We contend that each of these enduring legacies of activism make future human rights atrocities in Argentina highly unlikely. Further, we argue that because of these enormous gains, segments of the movement struggle to define themselves relative to the state, trying to avoid being captured by governmental interests and to maintain sufficient independence to effectively pressure the state.La década pasada ha visto un cambio sísmico en la habilidad de activistas argentinos por los derechos humanos de realizar metas largamente sostenidas, como las de llevar ante la justicia a los perpetradores y preservar la memoria de las víctimas de dictadura civil-estatal de los años setenta y ochenta. Las teorías de courageous resistance a la injusticia postulan que toda lucha por justicia produce cambios en el contexto, las redes y las personas, los cuales pueden mejorar la probabilidad de incidencia política prosocial para el futuro. En base a una variedad de evidencia, evaluamos el impacto de casi 40 años de activismo sostenido. Argumentamos que hay cambios resultantes en las esferas de 1) el contexto (instituciones legales y estatales nuevamente creadas y modificadas, cambios culturales y modificaciones al paisaje físico), 2) las relaciones entre activistas, la sociedad y el Estado argentino y 3) factores en la socialización que afectan las actitudes y el comportamiento de argentinos (cambios en las formas de referenciar, enseñar y conmemorar la época). Sostenemos que cada uno de estos importantes y duraderos legados de activismo hace que futuras atrocidades de derechos humanos en la Argentina sean altamente improbables. Además, argumentamos que, a causa de estos logros, sectores del movimiento se definen difícilmente con respecto al Estado, intentando evitar la captura por los intereses gubernamentales y mantener una independencia suficiente para presionar eficazmente al Estado

Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathway

DNA‐protein crosslinks (DPCs) obstruct essential DNA transactions, posing a serious threat to genome stability and functionality. DPCs are proteolytically processed in a ubiquitin‐ and DNA replication‐dependent manner by SPRTN and the proteasome but can also be resolved via targeted SUMOylation. However, the mechanistic basis of SUMO‐mediated DPC resolution and its interplay with replication‐coupled DPC repair remain unclear. Here, we show that the SUMO‐targeted ubiquitin ligase RNF4 defines a major pathway for ubiquitylation and proteasomal clearance of SUMOylated DPCs in the absence of DNA replication. Importantly, SUMO modifications of DPCs neither stimulate nor inhibit their rapid DNA replication‐coupled proteolysis. Instead, DPC SUMOylation provides a critical salvage mechanism to remove DPCs formed after DNA replication, as DPCs on duplex DNA do not activate interphase DNA damage checkpoints. Consequently, in the absence of the SUMO‐RNF4 pathway cells are able to enter mitosis with a high load of unresolved DPCs, leading to defective chromosome segregation and cell death. Collectively, these findings provide mechanistic insights into SUMO‐driven pathways underlying replication‐independent DPC resolution and highlight their critical importance in maintaining chromosome stability and cellular fitness