Сontrolled breeding of the psychrophilic strain G-034 VIZR of Trichoderma asperellum for fast crop residues’ polymers utilization and soil enhancement

Genus Trichoderma strains as the natural plant residues’ biodestructors, highly active antagonists of soil phytopathogens and phytoregulators with the widest range of optimum conditions for their development, are widely used in biologics development. Of particular importance in Russia’s northern regions, especially in winter crop cultivation, is the ability of a microorganism’s strain used in agro-technologies to maintain viability and target biological activity at low temperatures. In this connection, this work purpose is to select a psychrotolerant strain of T. asperellum for the rapid crop residues’ polymer utilization and soil enhancement at low temperature, as well as to evaluate its activity under laboratory and field conditions. In the work process, the following tasks were addressed: selecting psychrotolerant strains of T. asperellum with high cellulolytic activity; further controlled breeding of psychrophilic strains capable of rapid growth, active colonization of plant substrates and high sporulation at 4–8 °C; evaluating the target activity of the selected psychrophilic strain as a cellulolytic as well as antagonistic activity against cereal pathogens; obtaining laboratory samples of bioformulations by deep-surface cultivation on non-sterile peat and multirecycled wastes from the edible mushrooms production and assessing their efficacy in field small-plot trials. The methods for inoculum cultivation, sporulation capacity determination, modified wet chamber, estimating antagonistic activity and biologics’ quality, field small-plot trials management, quantitative estimates of biomass losses, cellulose and lignin content were used in the work. The active psychrophilic strain for the rapid crop residues’ polymer utilization and soil enhancement controlled breeding was selected during a four-step screening of 29 T. asperellum strains from All-Russian Research Institute of Plant Protection (VIZR) State Microorganisms’ Collection with high cellulolytic and antagonistic activities. In terms of linear growth rate, antagonistic and hyperparasitic activities at 4–8 °С, a high rate of wheat and maize stubble residues’ colonization, a perspective psychrophilic strain G-034 of T. asperellum was selected for developing the laboratory samples of biologics and for running field trials. In small-plot trials, the active maize crop residues’ decomposition under the T. asperellum G-034 influence was revealed, resulting in the complete loss of plant intact state in 12 months due to more than 80 % cellulose and 20 % lignin biodestruction. The maximum loss of maize crop residues biomass for 12 months was more than 70 %. The T. asperellum strain G-034 was active after field hibernation in an amount of ×104 cfu/g, resulting in a titer increase with seasonal temperature rising and the trophic base bioavailability growth

CHARACTERSTICS OF IMMUNE EFFECT OF BEMITHYL IN CHRONIC HEPATITIS

Abstract. Introduction of bemithyl into combined therapy of patients suffering chronic hepatitis allows toachieve a noticeable hepatoprotective effect. Apparently it is realized due to direct hepatotropic influence as well as through immunomodulatory action of the preparation directed at cellular immunity. At the same time, immunotropic action of bemithyl can be characterized as immunomodulatory one, which is confirmed by stimulation of T–cell immunity with some suppression of B–section and humoral immunity. An important factor of the immunotropic action of the drug is the absence of autoimmunization phenomena. Influence of bemithyl on macrophages section manifested by the decrease of liver tissue infiltration and simultaneous increase of blood monocyte count. At the same time, initial oxygen–dependent microbicidal activity of macrophages decreased a little, but stimulated indices increased. (Med. Immunol., 2005, vol.7, № 1, pp 93599

The diagnostics of single-phase capacitor induction motors by the level of external magnetic field, with allowance for technological and operational factors

Исмагилов Флюр Рашитович – докт. техн. наук, профессор, проректор, заведующий кафедрой «Электромеханика», Уфимский государственный авиационный технический университет, г. Уфа. Область научных интересов – электромеханические преобразователи энергии. Контактный телефон: 8 (347) 273-77-87. Ismagilov Flur Rashitovich – Doctor of Science (Engineering), Professor, pro-rector, Head of the Department of Electromechanics of Ufa State Aviation Technical University, Ufa. Research interests: electromechanical converters of energy. Tel.: 8 (347) 273-77-87. Хайруллин Ирек Ханифович – докт. техн. наук, профессор кафедры электромеханики, Уфимский государственный авиационный технический университет, г. Уфа. Область научных интересов – электромеханические преобразователи энергии. Контактный телефон: 8 (347) 273-77-87. Hairullin Irek Hanifovich – Doctor of Science (Engineering), Professor of the Department of Electromechanics of Ufa State Aviation Technical University. Research interests: electromechanical converters of energy. Tel.: 8 (347) 273-77-87. Пашали Диана Юрьевна – канд. техн. наук, доцент кафедры «Электромеханика», Уфимский государственный авиационный технический университет, г. Уфа. Область научных интересов – надежность электромеханических систем. Контактный телефон: 8-(917) 345-95-88. Pashali Diana Yurievna – Cand. Sc. (Engineering), an assistant professor of the Department of Electromechanics of Ufa State Aviation Technical University, Ufa. Research interests: electromechanical converters of energy. Tel.: 8 (917) 345-95-88. Бойкова Оксана Алексеевна – аспирант кафедры «Электромеханика», Уфимский государственный авиационный технический университет, г. Уфа. Область научных интересов – электромеханические преобразователи энергии. Контактный телефон: 8 (917) 400-52-30. Boykova Oksana Alexeyevna – a postgraduate student of the Department of Electromechanics of Ufa State Aviation Technical University, Ufa. Research interests: electromechanical converters of energy. Tel.: 8 (917) 400-52-30.Рассматриваются вопросы диагностики конденсаторных однофазных асинхронных двигателей по внешнему магнитному полю с учетом возможных отклонений размеров форм и взаимного расположения поверхностей активных частей, вызванных технологическими и эксплуатационными факторами. Обсуждаются полученные результаты, в том числе диагностические признаки и особенности практического применения метода. The problems of diagnostics of single-phase capacitor induction motors by the level of external magnetic field with allowance for possible deviations of the sizes of the forms and relative position of active parts of the surface, caused by technological and operational factors are analyzed. The results obtained, including diagnostic features and characteristics of the practical application of this method are discussed.Уфимский государственный авиационный технический университе

Antibiotic Activity of Actinobacteria from the Digestive Tract of Millipede <i>Nedyopus dawydoffiae</i> (Diplopoda)

Because of the spread of drug resistance, it is necessary to look for new antibiotics that are effective against pathogenic microorganisms. The purpose of this study was to analyse the species composition of actinobacteria isolated from the digestive tract of the millipedes Nedyopus dawydoffiae and to determine their antimicrobial properties. Species identification was carried out on the basis of the morphological and culture properties and the sequence of the 16S rRNA gene. Actinobacteria were grown in different liquid media. Antibiotic properties were determined against some Gram-positive and Gram-negative bacteria as well as fungi. Of the 15 isolated strains, 13 have antibiotic activity against Gram-positive bacteria (including methicillin-resistant Staphylococcus aureus&#8212;MRSA) and fungi, but there was no antibiotic activity against Gram-negative test strains Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. It was established that antibiotic-producing actinobacteria belong to eight species of the genus Streptomyces. Depending on the nutrient medium, actinobacteria demonstrate different antimicrobial activities. As an example, S. hydrogenans shows that even strains selected in one population differ by the range of antimicrobial activity and the level of biosynthesis. Since the antibiotic production is considered as a feature for species competition in the microbiota community, the variability of antibiotic production among different strains of the same species is an adaptive characteristic for the competition in millipedes&#8217; digestive tract community