Light sheet adaptive optics microscope for 3D live imaging

Optical microscopy is still the main research tool for many biological studies. Indeed with the advent of genetic manipulation and specifically, the use of fluorescent protein expressing in animals and plants it has actually seen a renaissance in the past ten years, in particular with the development of novel techniques such as CARS, PALM, STORM, STED and SPIM. In all of microscopy methods one has to look through the sample at some point. The sample thus adds an additional and uncontrolled optical path, which leads to aberrations in the final image. Adaptive optics (AO) is a way of removing these unwanted aberrations which can cause image degradation and even potentially artifacts within the image. This thesis is concerned with the implementation of AO in non scanning microscopes and presents some novel methods both in wavefront sensored and sensorless configurations. A first implementation of AO on the emission path of a light sheet microscope is also presented

Spectral index selection method for remote moisture sensing under challenging illumination conditions

Remote sensing using passive solar illumination in the Short-Wave Infrared spectrum is exposed to strong intensity variation in the spectral bands due to atmospheric changing conditions and spectral absorption. More robust spectral analysis methods, insensitive to these effects, are increasingly required to improve the accuracy of the data analysis in the field and extend the use of the system to “non ideal” illumination condition. A computational hyperspectral image analysis method (named HIAM) for deriving optimal reflectance indices for use in remote sensing of soil moisture content is detailed and demonstrated. Using histogram analysis of hyperspectral images of wet and dry soil, contrast ratios and wavelength pairings were tested to find a suitable spectral index to recover soil moisture content. Measurements of local soil samples under laboratory and field conditions have been used to demonstrate the robustness of the index to varying lighting conditions, while publicly available databases have been used to test across a selection of soil classes. In both cases, the moisture was recovered with RMS error better than 5%. As the method is independent of material type, this method has the potential to also be applied across a variety of biological and man-made samples

Integrated fiber optic spectrally resolved downwelling irradiance sensor for pushbroom spectrometers

We present an integrated fiber optic spectrally resolved downwelling irradiance sensor for pushbroom hyperspectral imagers. The system comprises of a cosine corrector and custom fiber patch cables, collecting the ambient light in a large solid angle and feeding it directly to the entrance slit of the spectrometer. The system enables simultaneous measurement of downwelling and upwelling irradiance using the main hyperspectral camera sensor. As a demonstration, the spectral reflectance of a soil sample was measured with a RMSE of 8.4%, a significant improvement on the RMSE of 54% found without correction. At a weight of approximately 10 grams, this system provides a substantial weight saving over standalone incident light sensing instruments

Topology Optimization of a Single-Point Diamond-Turning Fixture for a Deployable Primary Mirror Telescope

CubeSats, known for their compact size and cost effectiveness, have gained significant popularity. However, their limited size imposes restrictions on the optical aperture and, consequently, the Ground Resolution Distance in Earth Observation missions. To overcome this limitation, the concept of deployable optical payloads with segmented primary mirrors which can unfold like petals has emerged, enabling larger synthetic apertures and enhanced spatial resolution. This study explores the potential benefits of leveraging Additive Manufacturing (AM) and Topology Optimization (TO) in the realm of ultra-precision machining, specifically single-point diamond machining. The goal is to reduce fixture weight while improving stiffness to minimize deformations caused by rotational and cutting forces which compromise optical performance. Through Finite Element Analysis, this research compares conventionally machined fixtures with those produced using AM and TO techniques. The results reveal that concept designs created via TO can achieve a remarkable 68% reduction in weight. This reduction makes the assembly, including the machining fixture and 12 U deployable segments, manageable by a single operator without the need for specialized lifting equipment. Moreover, these innovative designs lead to substantial reductions of up to 86% and 51% in deformation induced by rotational and cutting forces, respectively

Design of freeform diffraction gratings: performance, limitations and potential applications

Spectroscopy is a key technique in astronomy and nowadays most major telescopes include at least one spectrograph in their instrument suite. The dispersive element is one of the most important components and it defines the pupil size, spectral resolution and efficiency. Different types of dispersive elements have been developed including prisms, grisms, VPH and echelle gratings. In this paper, we investigate the design and optimization possibilities offered by metallic freeform gratings using diamond machining techniques. The incorporation of power in a diffraction grating enables several functionalities within the same optical component, such as the combination of dispersion, focusing and field reformat. The resulting benefit is a reduction in the number of surfaces and therefore, an improvement in the throughput. Freeform surfaces are also interesting for their enhanced optical performance by allowing extra degree of freedom in the optimization. These degrees of freedom include the shape of the substrate but also additional parameters such as the pitch or the number of blaze angle. Freeform gratings used as single optical component systems also present some limitations such as the trade-off between optical quality versus field of view or the spectral range versus spectral resolution. This paper discusses the possibility offered by the design of freeform gratings for low to medium spectral resolution, in the visible and near-infrared, for potential applications in ultra-compact integral field spectrographs

The HR image slicer for GNIRS at Gemini North: optical design and performance

GNIRS (Gemini Near-InfraRed Spectrograph) is a multi-function spectrograph at Gemini North telescope offering four observational modes in the spectral range of 0.8 to 5.4 µm. It provides 2-pixel spectral resolutions from 1,200 up to 18,0000 and has single disperser and cross-disperser modes yielding simultaneous spectral bandwidths from 40 nm to 1,650 nm. GNIRS presented three existing modes: long-slit (50-100" slit), cross-dispersed (5-7" slit) and low resolution (LR) Integral Field Unit (IFU) (3.15" x 4.80") and it is now being upgraded with a fourth mode allowing high resolution (HR) IFU spectroscopy using an image slicer optimised for fully adaptively corrected images over a field of view of 2.25 arcsec2 (1.80" x 1.25") covered by 25 slices of 410 µm width offering a spatial sampling of 0.05 x 0.05 arscec2 with a diffraction limited optical quality. The proposed layout meets specifications and some challenging design constraints: it shall be contained within the same envelope defined by the LR image slicer (0.1 x 0.2 x 0.1 m3 ), the input and output focal-ratios of both image slicers shall be the same and at exact positions but providing different anamorphic magnifications and preserving the optical quality. The length of the generated slit will be similar to the length of the slit in long-slit mode to maximise detector use and avoid vignetting. This communication presents the optical design and performance of the high resolution image slicer compliant with all specifications and constraints and it shows some design adaptations adopted in order to facilitate its manufacturing in metal at Durham University

Design, Manufacture, and Evaluation of Prototype Telescope Windows for Use as Low Vision Aids

Pixellated Optics, a class of optical devices which preserve phase front continuity only over small sub areas of the device, allow for a range of uses that would not otherwise be possible. One potential use is as Low Vision Aids (LVAs), where they are hoped to combine the function and performance of existing devices with the size and comfort of conventional eyewear. For these devices a Generalised Confocal Lenslet Array (GCLA) is designed to magnify object space, creating the effect of traditional refracting telescope within a thin, planar device. By creating a device that is appreciably thinner than existing LVA telescopes it is hoped that the comfort for the wearer will be increased. We have developed a series of prototype GLCA-based devices to examine their real-world performance, focussing on the resolution, magnification and clarity of image attainable through the devices. It is hoped that these will form the basis for a future LVA devices. This development has required novel manufacturing techniques and a phased development approach centred on maximising performance. Presented here will be an overview of the development so far, alongside the performance of the latest devices

Steering Mirror System with Closed-Loop Feedback for Free-Space Optical Communication Terminals

Precision beam pointing plays a critical role in free-space optical communications terminals in uplink, downlink and inter-satellite link scenarios. Among the various methods of beam steering, the use of fast steering mirrors (FSM) is widely adopted, with many commercial solutions employing diverse technologies, particularly focusing on small, high-bandwidth mirrors. This paper introduces a method using lightweight, commercial off-the-shelf components to construct a custom closed-loop steering mirror platform, suitable for mirror apertures exceeding 100 mm. The approach involves integrating optical encoders into two off-the-shelf open-loop actuators. These encoders read the signal reflected on purposefully diamond-machined knurled screw knobs, providing maximum contrast between light and dark lines. The resulting steering mirror has the potential to complement or replace FSM in applications requiring a larger stroke, at the expense of motion speed. In the presented setup, the mirror tilt resolution achieved based on the encoder closed-loop signal feedback is 45 μrad, with a mean slew rate of 1.5 mrad/s. Importantly, the steering assembly is self-locking, requiring no power to maintain a steady pointing angle. Using the mirror to actively correct for a constantly moving incoming beam, a 5-fold increase in concentration of the beam spot on the center of the detector was obtained compared to a fixed position mirror, demonstrating the mirrors ability to correct for satellite platform jitter and drift

Closed loop adaptive optics with a laser guide star for biological light microscopy

We report on the development of a widefield microscope that achieves adaptive optics correction through the use of a wavefront sensor observing an artificial laser guide star induced within the sample. By generating this guide star at arbitrary positions and depths within the sample we allow the delivery of high-resolution images. This approach delivers much faster AO correction than image optimization techniques, and allows the use of AO with fluorescent imaging modalities without generating excessive photo-toxic damage in the sample, or inducing significant photo-bleaching in the flurophore molecules

Realtime wavefront sensing in a SPIM microscope, and active aberration tracking

Adaptive optics (AO) can potentially allow high resolution imaging deep inside living tissue, mitigating against the loss of resolution due to aberrations caused by overlying tissue. Closed-loop AO correction is particularly attractive for moving tissue and spatially varying aberrations, but this requires direct wavefront sensing, which in turn requires suitable "guide stars" for use as wavefront references. We present a novel method for generating an orthogonally illuminated guide star suitable for direct wavefront sensing in a wide range of fluorescent biological structures, along with results demonstrating its use for measuring time-varying aberrations, in vivo