Serendipity observations of far infrared cirrus emission in the Spitzer Infrared Nearby Galaxies Survey: Analysis of far-infrared correlations

We present an analysis of far-infrared dust emission from diffuse cirrus clouds. This study is based on serendipitous observations at 160 microns at high galactic latitude with the Multiband Imaging Photometer (MIPS) onboard the Spitzer Space Telescope by the Spitzer Infrared Nearby Galaxies Survey (SINGS). These observations are complemented with IRIS data at 100 and 60 microns and constitute one of the most sensitive and unbiased samples of far infrared observations at small scale of diffuse interstellar clouds. Outside regions dominated by the cosmic infrared background fluctuations, we observe a substantial scatter in the 160/100 colors from cirrus emission. We compared the 160/100 color variations to 60/100 colors in the same fields and find a trend of decreasing 60/100 with increasing 160/100. This trend can not be accounted for by current dust models by changing solely the interstellar radiation field. It requires a significant change of dust properties such as grain size distribution or emissivity or a mixing of clouds in different physical conditions along the line of sight. These variations are important as a potential confusing foreground for extragalactic studies.Comment: 25 pages, 7 figures, 2 tables, accepted to Ap

Are we seeing accretion flows in a 250kpc-sized Ly-alpha halo at z=3?

Using MUSE on the ESO-VLT, we obtained a 4 hour exposure of the z=3.12 radio galaxy MRC0316-257. We detect features down to ~10^-19 erg/s/cm^2/arcsec^2 with the highest surface brightness regions reaching more than a factor of 100 higher. We find Ly-alpha emission out to ~250 kpc in projection from the active galactic nucleus (AGN). The emission shows arc-like morphologies arising at 150-250 kpc from the nucleus in projection with the connected filamentary structures reaching down into the circum-nuclear region. The most distant arc is offset by 700 km/s relative to circum-nuclear HeII 1640 emission, which we assume to be at the systemic velocity. As we probe emission closer to the nucleus, the filamentary emission narrows in projection on the sky, the relative velocity decreases to ~250 km/s, and line full-width at half maximum range from 300-700 km/s. From UV line ratios, the emission on scales of 10s of kpc from the nucleus along a wide angle in the direction of the radio jets is clearly excited by the radio jets and ionizing radiation of the AGN. Assuming ionization equilibrium, the more extended emission outside of the axis of the jet direction would require 100% or more illumination to explain the observed surface brightness. High speed (>300 km/s) shocks into rare gas would provide sufficiently high surface brightness. We discuss the possibility that the arcs of Ly-alpha emission represent accretion shocks and the filamentary emission represent gas flows into the halo, and compare our results with gas accretion simulations.Comment: 4 pages, 2 figures, 1 table, A&A letters accepte

Magnetic field morphology in nearby molecular clouds as revealed by starlight and submillimetre polarization

Within four nearby (d < 160 pc) molecular clouds, we statistically evaluate the structure of the interstellar magnetic field, projected on the plane of the sky and integrated along the line of sight, as inferred from the polarized thermal emission of Galactic dust observed by Planck at 353 GHz and from the optical and NIR polarization of background starlight. We compare the dispersion of the field orientation directly in vicinities with an area equivalent to that subtended by the Planck effective beam at 353 GHz (10') and using the second-order structure functions of the field orientation angles. We find that the average dispersion of the starlight-inferred field orientations within 10'-diameter vicinities is less than 20 deg, and that at these scales the mean field orientation is on average within 5 deg of that inferred from the submillimetre polarization observations in the considered regions. We also find that the dispersion of starlight polarization orientations and the polarization fractions within these vicinities are well reproduced by a Gaussian model of the turbulent structure of the magnetic field, in agreement with the findings reported by the Planck collaboration at scales greater than 10' and for comparable column densities. At scales greater than 10', we find differences of up to 14.7 deg between the second-order structure functions obtained from starlight and submillimetre polarization observations in the same positions in the plane of the sky, but comparison with a Gaussian model of the turbulent structure of the magnetic field indicates that these differences are small and are consistent with the difference in angular resolution between both techniques.Comment: 15 pages, 10 figures, submitted to A&

Structure formation in a colliding flow: The Herschel view of the Draco nebula

The Draco nebula is a high Galactic latitude interstellar cloud likely to have been formed by the collision of a Galactic halo cloud entering the disk of the Milky Way. Such conditions are ideal to study the formation of cold and dense gas in colliding flows of warm gas. We present Herschel-SPIRE observations that reveal the fragmented structure of the interface between the infalling cloud and the Galactic layer. This front is characterized by a Rayleigh-Taylor instability structure. From the determination of the typical length of the periodic structure (2.2 pc) we estimated the gas kinematic viscosity and the turbulence dissipation scale (0.1 pc) that is compatible with that expected if ambipolar diffusion is the main mechanism of energy dissipation in the WNM. The small-scale structures of the nebula are typical of that seen in some molecular clouds. The gas density has a log-normal distribution with an average value of $10^3$ cm$^{-3}$. The size of the structures is 0.1-0.2 pc but this estimate is limited by the resolution of the observations. The mass ranges from 0.2 to 20 M$_{\odot}$ and the distribution of the more massive clumps follows a power law $dN/d\log(M) \sim M^{-1.4}$. We identify a mass-size relation with the same exponent as that found in GMCs ($M\sim L^{2.3}$) but only 15% of the mass of the cloud is in gravitationally bound structures. We conclude that the increase of pressure in the collision is strong enough to trigger the WNM-CNM transition caused by the interplay between turbulence and thermal instability as self-gravity is not dominating the dynamics.Comment: 16 pages, A&A, in pres

Accurate Galactic 21-cm H I measurements with the NRAO Green Bank Telescope

Aims: We devise a data reduction and calibration system for producing highly-accurate 21-cm H I spectra from the Green Bank Telescope (GBT) of the NRAO. Methods: A theoretical analysis of the all-sky response of the GBT at 21 cm is made, augmented by extensive maps of the far sidelobes. Observations of radio sources and the Moon are made to check the resulting aperture and main beam efficiencies. Results: The all-sky model made for the response of the GBT at 21 cm is used to correct for "stray" 21-cm radiation reaching the receiver through the sidelobes rather than the main beam. This reduces systematic errors in 21-cm measurements by about an order of magnitude, allowing accurate 21-cm H I spectra to be made at about 9' angular resolution with the GBT. At this resolution the procedures discussed here allow for measurement of total integrated Galactic H I line emission, W, with errors of 3 K km s^-1, equivalent to errors in optically thin N_HI of 5 x 10^18 cm^-2.Comment: 49 pages, 25 figures; A&A, in pres

Sensorimotor Transformations in the Zebrafish Auditory System

Organisms use their sensory systems to acquire information from their environment and integrate this information to produce relevant behaviors. Nevertheless, how sensory information is converted into adequate motor patterns in the brain remains an open question. Here, we addressed this question using two-photon and light-sheet calcium imaging in intact, behaving zebrafish larvae. We monitored neural activity elicited by auditory stimuli while simultaneously recording tail movements. We observed a spatial organization of neural activity according to four different response profiles (frequency tuning curves), suggesting a low-dimensional representation of frequency information, maintained throughout the development of the larvae. Low frequencies (150–450 Hz) were locally processed in the hindbrain and elicited motor behaviors. In contrast, higher frequencies (900–1,000 Hz) rarely induced motor behaviors and were also represented in the midbrain. Finally, we found that the sensorimotor transformations in the zebrafish auditory system are a continuous and gradual process that involves the temporal integration of the sensory response in order to generate a motor behavior.Fil: Privat, Martin. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Romano, Sebastián Alejo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Pietri, Thomas. Centre National de la Recherche Scientifique; Francia. Inserm; FranciaFil: Jouary, Adrien. Champalimaud Centre For The Unknown; Portugal. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Boulanger Weill, Jonathan. Centre National de la Recherche Scientifique; Francia. Inserm; FranciaFil: Elbaz, Nicolas. Inserm; Francia. Centre National de la Recherche Scientifique; FranciaFil: Duchemin, Auriane. Centre National de la Recherche Scientifique; Francia. Inserm; FranciaFil: Soares, Daphne. New Jersey Institute of Technology; Estados UnidosFil: Sumbre, Germán. Centre National de la Recherche Scientifique; Francia. Inserm; Franci

Protein crystals in adenovirus type 5-infected cells: requirements for intranuclear crystallogenesis, structural and functional analysis

Intranuclear crystalline inclusions have been observed in the nucleus of epithelial cells infected with Adenovirus serotype 5 (Ad5) at late steps of the virus life cycle. Using immuno-electron microscopy and confocal microscopy of cells infected with various Ad5 recombinants modified in their penton base or fiber domains, we found that these inclusions represented crystals of penton capsomers, the heteromeric capsid protein formed of penton base and fiber subunits. The occurrence of protein crystals within the nucleus of infected cells required the integrity of the fiber knob and part of the shaft domain. In the knob domain, the region overlapping residues 489–492 in the FG loop was found to be essential for crystal formation. In the shaft, a large deletion of repeats 4 to 16 had no detrimental effect on crystal inclusions, whereas deletion of repeats 8 to 21 abolished crystal formation without altering the level of fiber protein expression. This suggested a crucial role of the five penultimate repeats in the crystallisation process. Chimeric pentons made of Ad5 penton base and fiber domains from different serotypes were analyzed with respect to crystal formation. No crystal was found when fiber consisted of shaft (S) from Ad5 and knob (K) from Ad3 (heterotypic S5-K3 fiber), but occurred with homotypic S3K3 fiber. However, less regular crystals were observed with homotypic S35-K35 fiber. TB5, a monoclonal antibody directed against the Ad5 fiber knob was found by immunofluorescence microscopy to react with high efficiency with the intranuclear protein crystals in situ. Data obtained with Ad fiber mutants indicated that the absence of crystalline inclusions correlated with a lower infectivity and/or lower yields of virus progeny, suggesting that the protein crystals might be involved in virion assembly. Thus, we propose that TB5 staining of Ad-infected 293 cells can be used as a prognostic assay for the viability and productivity of fiber-modified Ad5 vectors

Some empirical estimates of the H2 formation rate in photon-dominated regions

We combine recent ISO observations of the vibrational ground state lines of H2 towards Photon-Dominated Regions (PDRs) with observations of vibrationally excited states made with ground-based telescopes in order to constrain the formation rate of H2 on grain surfaces under the physical conditions in the layers responsible for H2 emission. We use steady state PDR models in order to examine the sensitivity of different H2 line ratios to the H2 formation rate Rf. We show that the ratio of the 0-0 S(3) to the 1-0 S(1) line increases with Rf but that one requires independent estimates of the radiation field incident upon the PDR and the density in order to infer Rf from the H2 line data. We confirm the earlier result of Habart et al. (2003) that the H2 formation rate in regions of moderate excitation such as Oph W, S140 and IC 63 is a factor of 5 times larger than the standard rate inferred from UV observations of diffuse clouds. On the other hand, towards regions of higher radiation field such as the Orion Bar and NGC 2023, we derive H2 formation rates consistent with the standard value. We find also a correlation between the H2 1-0 S(1) line and PAH emission suggesting that Rf scales with the PAH abundance. With the aim of explaining these results, we consider some empirical models of the H2 formation process. Here we consider both formation on big (a~0.1 microns) and small (a~10 Angstroms) grains by either direct recombination from the gas phase or recombination of physisorbed H atoms with atoms in a chemisorbed site. We conclude that indirect chemisorption is most promising in PDRs. Moreover small grains which dominate the total grain surface and spend most of their time at relatively low temperatures may be the most promising surface for forming H2 in PDRs.Comment: A&A in press, 16 pages, 5 figure

Properties of dust in the high-latitude translucent cloud L1780 I: Spatially distinct dust populations and increased dust emissivity from ISO observations

We have analyzed the properties of dust in the high galactic latitude translucent cloud Lynds 1780 using ISOPHOT maps at 100 and 200 micrometers and raster scans at 60, 80, 100, 120, 150 and 200 micrometers. In far-infrared (FIR) emission, the cloud has a single core that coincides with the maxima of visual extinction and 200um optical depth. At the resolution of 3.0 arcmin, the maximum visual extinction is 4.0 mag. At the cloud core, the minimum temperature and the maximum 200um optical depth are 14.9+/-0.4 K and 2.0+/-0.2x10^{-3}, respectively, at the resolution of 1.5 arcmin. The cloud mass is estimated to be 18M_{SUN}. The FIR observations, combined with IRAS observations, suggest the presence of different, spatially distinct dust grain populations in the cloud: the FIR core region is the realm of the "classical" large grains, whereas the very small grains and the PAHs have separate maxima on the Eastern side of the cold core, towards the "tail" of this cometary-shaped cloud. The color ratios indicate an overabundance of PAHs and VSGs in L1780. Our FIR observations combined with the optical extinction data indicate an increase of the emissivity of the big grain dust component in the cold core, suggesting grain coagulation or some other change in the properties of the large grains. Based on our observations, we also address the question, to what extent the 80um emission and even the 100um and the 120um emission contain a contribution from the small-grain component.Comment: 12 pages, 9 figures, minor changes, one table adde