Diversity of Lebanese royal irises, illustrated by some of the studied taxa.

<p>A: <i>Iris sofarana</i> subsp. <i>sofarana</i>, B: <i>I</i>. <i>sofarana</i> subsp. <i>kasruwana</i>, C: <i>I</i>. <i>cedreti</i>, D: <i>I</i>. <i>westii</i>, E: <i>I</i>. <i>bismarckiana</i>, F: <i>I</i>. <i>lortetii</i>, G: <i>I</i>. <i>antilibanotica</i>. Photographs from M. Bou Dagher-Kharrat</p

Main data on karyotype features of investigated <i>Iris</i> taxa.

<p>Main data on karyotype features of investigated <i>Iris</i> taxa.</p

Unlocking the Karyological and Cytogenetic Diversity of <i>Iris</i> from Lebanon: <i>Oncocyclus</i> Section Shows a Distinctive Profile and Relative Stasis during Its Continental Radiation - Fig 2

<p><b>Metaphase chromosome plates and Idiograms of <i>Iris</i> taxa A</b>–<b>K</b>: Metaphase chromosome plates of <i>Iris</i> taxa after double target FISH with 5S (green signals) and 18-26S rDNA (red signals) probes. C1 represents CMA staining (yellow signals). <b>A’</b>–<b>K’</b>: Idiograms with location of 5S (green) and 18-26S rDNA (red) rRNA genes. <b>A’</b>: <i>I</i>. <i>sofarana</i> subsp. <i>sofarana</i> (Falougha), <b>B’</b>: <i>I</i>. <i>sofarana</i> subsp. <i>sofarana</i> (Hazzerta) <b>C’</b>: <i>I</i>. <i>sofarana</i> subsp. <i>kasruwana</i>, <b>D’</b>: <i>I</i>. <i>cedreti</i>, <b>E’</b>: <i>I</i>. <i>westii</i>, <b>F’</b>: <i>I</i>. <i>bismarckiana</i>, <b>G’</b>: <i>I</i>. <i>lortetii</i>, <b>H’</b>: <i>I</i>. <i>antilibanotica</i>, <b>I’</b>: <i>I</i>. <i>persica</i>, <b>J’</b>: <i>I</i>. <i>unguicularis</i> var. <i>cretensis</i>, <b>K’</b>: <i>I</i>. <i>mesopotamica</i>. Scale bar 10 <i>μ</i>m.</p

Synthesis of results concerning molecular cytogenetic approach of investigated <i>Iris</i> taxa.

<p>Synthesis of results concerning molecular cytogenetic approach of investigated <i>Iris</i> taxa.</p

Geographic distribution of mtDNA haplogroups.

<p>Frequencies distribution from the current study and from the published data <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054616#pone.0054616-Gonzalez1" target="_blank">[30]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054616#pone.0054616-AlZahery1" target="_blank">[31]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054616#pone.0054616-Pereira1" target="_blank">[35]</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054616#pone.0054616-Cerny1" target="_blank">[48]</a> as reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054616#pone-0054616-t001" target="_blank">Table 1</a>.</p

mtDNA vs. Y-chromosome AMOVA results contrasting mtDNA and Y dendrogram-based classifications.

<p>mtDNA vs. Y-chromosome AMOVA results contrasting mtDNA and Y dendrogram-based classifications.</p

mtDNA Haplogroup frequencies of 1509 newly sequenced Levantine samples and 3665 samples collected from the literature.

<p>mtDNA Haplogroup frequencies of 1509 newly sequenced Levantine samples and 3665 samples collected from the literature.</p

Heatmap of the Y(Y+mtDNA) distances.

<p>The heatmap shows the normalized ratio of the Y <i>F_ST</i> distance with respect to the total distance (Y <i>R_ST</i>+mtDNA <i>F_ST</i> distances). The dendrograms are obtained using complete linkage hierarchical clustering with the Euclidean distance measure.</p

Populations comparison based on mtDNA haplogroups.

<p>a) Principal Component Analysis of relative frequencies of haplogroups within populations, b) with mean-linkage (UPGMA) dendrogram determined from Euclidean distances.</p

Nonmetric Multidimensional Scaling.

<p>a) mtDNA <i>F_ST</i> and b) Y-STR <i>R_ST</i> distances with c) mtDNA <i>F_ST</i> and d) Y-STR <i>R_ST</i> mean-linkage dendrogram.</p