Priprava vina na niskoj temperaturi s pomoću kvasca imobiliziranog na pivskom tropu bez lignina

Preservation, risk of contamination, transportation and storage costs (e.g. refrigeration) are the main problems associated with wet, active starter cultures such as yeast. To deal with these aspects, drying of commercial cultures is required. A thermally dried biocatalyst has been prepared by immobilization of the psychrotolerant yeast strain Saccharomyces cerevisiae AXAZ-1 on delignified brewer’s spent grains, followed by simple thermal drying or by air stream at 30 and 35 °C. Repeated batch fermentations of grape must (11.5 °Be, corresponding to 196 g/L of fermentable sugar) using the dried biocatalysts were performed at successively reduced temperatures (15, 10 and 5 °C). The fermented samples were analyzed for residual sugar, alcohol and free cell concentrations. The alcohol yield, alcohol productivity and sugar conversion were calculated in order to estimate the optimum conditions of drying. The volatile constituents in the produced wines were analysed by GC analysis. The results showed that drying of immobilized cells by the proposed techniques did not affect their viability and fermentative activity. High alcohol productivity, increased esters and lower concentrations of higher alcohols obtained by low-temperature fermentation using the dried biocatalysts indicate a potential improvement of product quality.Konzerviranje, rizik od onečišćenja, te cijena transporta i skladištenja (npr. hlađenja) najčešći su problemi pri korištenju vlažnih, aktivnih starter-kultura, tj. kvasaca. Stoga komercijalne kulture kvasaca treba osušiti. Sušeni je biokatalizator pripremljen imobilizacijom psihrotolerantnoga soja kvasca Saccharomyces cerevisiae AXAZ-1 na pivskom tropu, iz kojeg je prethodno uklonjen lignin, a zatim sušenjem toplinom ili strujom zraka pri 30 i 35 °C. Pomoću tako pripremljenog kvasca uspješno je provedeno nekoliko uzastopnih šaržnih postupaka vrenja mošta (11,5 °Be, proporcionalno 196 g/L fermentabilnog šećera) pri nižim temperaturama (15, 10 i 5 °C). Ispitana je koncentracija preostalog šećera, dobivenog alkohola i slobodnih stanica u fermentiranim uzorcima. Da bi se procijenili optimalni uvjeti sušenja, izračunati su ovi parametri: udio i produktivnost alkohola te konverzija šećera. Volatilni su sastojci vina analizirani plinskom kromatografijom. Rezultati pokazuju da sušenje imobiliziranih stanica kvasca predloženim metodama ne utječe na njegovu vitalnost i fermentacijsku aktivnost. Kakvoća se proizvoda može poboljšati korištenjem sušenih stanica kvasca pri nižim temperaturama, što je uočeno iz većeg udjela alkohola, veće koncentracije estera i manje koncentracije viših alkohola u vinu

Novel cheese production by incorporation of sea buckthorn berries (Hippophae rhamnoides L.) supported probiotic cells

Sea buckhorn berries (SBB, Hippophae rhamnoides L.) were used as a novel support for immobilisation of the probiotic strain L. casei ATCC 393. The biocatalyst was employed for the development of a bioprocess for feta-type cheese production that leads to the improvement of quality and nutritional characteristics. Specifically, the effect of SBB supported biocatalyst on the microbiological safety and aroma profile of cheeses was compared with control samples and cheeses containing free L. casei cells showing superior properties with increased content of esters, alcohols and terpenes. The presence of probiotic culture, either in free or immobilised form, affected positively the physicochemical characteristics of cheeses during ripening. Cheeses with SBB had enriched aroma with terpenes and carbonyl compounds and higher probiotic cell population. The proposed bioprocess of employing SBB as immobilisation carrier shows great potential for commercialisation and application in manufacturing of probiotic functional dairy food.Fil: Terpou, Antonia. University of Patras; GreciaFil: Gialleli, Angelika Ioanna. University of Patras; GreciaFil: Bosnea, Loulouda. University of Patras; GreciaFil: Kanellaki, Maria. University of Patras; GreciaFil: Koutinas, Athanasios A.. University of Patras; GreciaFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin

Low-Temperature winemaking by thermally dried immobilized yeast on delignified brewer's spent grains

Preservation, risk of contamination, transportation and storage costs (e.g. refrigeration) are the main problems associated with wet, active starter cultures such as yeast. To deal with these aspects, drying of commercial cultures is required. A thermally dried biocatalyst has been prepared by immobilization of the psychrotolerant yeast strain Saccharomyces cerevisiae AXAZ-1 on delignified brewer's spent grains, followed by simple thermal drying or by air stream at 30 and 35 °C. Repeated batch fermentations of grape must (11.5 °Be, corresponding to 196 g/L of fermentable sugar) using the dried biocatalysts were performed at successively reduced temperatures (15, 10 and 5 °C). The fermented samples were analyzed for residual sugar, alcohol and free cell concentrations. The alcohol yield, alcohol productivity and sugar conversion were calculated in order to estimate the optimum conditions of drying. The volatile constituents in the produced wines were analysed by GC analysis. The results showed that drying of immobilized cells by the proposed techniques did not affect their viability and fermentative activity. High alcohol productivity, increased esters and lower concentrations of higher alcohols obtained by low-temperature fermentation using the dried biocatalysts indicate a potential improvement of product qualit

Effect of Dough-Related Parameters on the Antimold Activity of <i>Wickerhamomyces anomalus</i> Strains and Mold-Free Shelf Life of Bread

The aim of the present study was to assess the antimold capacity of three Wickerhamomyces anomalus strains, both in vitro and in situ, and to identify the responsible volatile organic compounds. For that purpose, two substrates were applied; the former included brain heart infusion broth, adjusted to six initial pH values (3.5, 4.0, 4.5, 5.0, 5.5, 6.0) and supplemented with six different NaCl concentrations (0.0%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%), while the latter was a liquid dough, fortified with the six aforementioned NaCl concentrations. After a 24 h incubation at 30 °C, the maximum antimold activity was quantified for all strains at 5120 AU/mL, obtained under different combinations of initial pH value and NaCl concentration. A total of twelve volatile compounds were detected; ethanol, ethyl acetate, isoamyl alcohol and isoamyl acetate were produced by all strains. On the contrary, butanoic acid-ethyl ester, acetic acid-butyl ester, ethyl caprylate, 3-methyl-butanoic acid, 2,4-di-tert-butyl-phenol, benzaldehyde, nonanal and octanal were occasionally produced. All compounds exhibited antimold activity; the lower MIC was observed for 2,4-di-tert-butyl-phenol and benzaldehyde (0.04 and 0.06 μL/mL of headspace, respectively), while the higher MIC was observed for butanoic acid-ethyl ester and ethyl caprylate (5.14 and 6.24 μL/mL of headspace, respectively). The experimental breads made with W. anomalus strains LQC 10353, 10346 and 10360 gained an additional period of 9, 10 and 30 days of mold-free shelf life, compared to the control made by commercially available baker’s yeast. Co-culture of the W. anomalus strains with baker’s yeast did not alter the shelf-life extension, indicating the suitability of these strains as adjunct cultures