DYNAMICS OF SORPTION OF TRANSITION AND ALKALINE EARTH METALS IONS BY SULFOETHYLPOLYSTYRENE 1.0

In this research, the degrees of extraction of transition and alkaline-earth metal ions by sulfoethylpolyaminostyrene with a degree of substitution 1.0 were determined under dynamic conditions. The dynamic capacitance of the ions is estimated. It is shown that the sorbent is selective for silver (I) and copper (II) ions.Работа выполнена при финансовой поддержке постановления № 211 Правительства Российской Федерации, контракт № 02.A03.21.0006

EFFECT OF THE WEIGHT OF SULFOETHYLPOLYSTYRENE ON THE SORPTION OF SILVER(I)AND COPPER(II) IN DYNAMIC CONDITIONS

In this research influence of weight on sorption of sulfoethylpolystyrene in dynamic conditions was studied. Selectivity coefficients KAg/Cuwere determined for sorbents with degree of substitution equal to 0.7. It was showed,that the dynamic capacity of the sorbent for ions of copper (II) and silver (I) increased with increasing of sorbent mass, but the selectivity of silver ions (I) and copper (II) was reduced.Исследование выполнено при финансовой поддержке РФФИ в рамках научного проекта № 16-33-00110 мол_а

Уровень нейротрофинов в головном мозге у мышей с нокаутом гена урокиназы при экспериментальной меланоме и коморбидной патологии

The objective was to evaluate the levels of neurotrophins in the brain of mice with urokinase (uPA) gene knockout, carriers of B16/F10 melanoma developing in presence of comorbid pathology – chronic neurogenic pain (CNP).Methods and materials. The study included female mice of two strains: С57ВL/6 (n=40) and C57BL/6-PlautmI.IBug-ThisPlau6FDhu/GFDhu (n=28). In the main groups, CNP was created by the bilateral sciatic nerve ligation, with В16/F10 melanoma transplanted under the skin of the back 2 weeks after. The comparison groups included sham operated animals with melanoma transplantation, the control groups – sham operated animals and animals with CNP. Mice were decapitated on day 21 of the tumor growth, and the brain levels of brain neurotrophic factor (BDNF); nerve growth factor (NGF), neurotrophins 3 (NT3) and 4 (NT4) were studied by ELISA.Results. The brain of mice with uPA gene knockout demonstrated higher levels of NT3 (by 1.3 times (p=0.0146)), NT4 (by 2.6 times) and NGF-β (by 1.9 times (p=0.0021)) and lower BDNF (by 1.7 times (p=0.0203)), compared to mice without knockout. Cerebral reduction of NGF-β was a nonspecific brain response to CNP and neoplastic growth in female mice, enhanced in the combination of the pathological factors. Greater stimulation of subcutaneous melanoma growth in female mice with uPA knockout under the influence of CNP combined with a 2-fold decrease in levels of NT3 and BDNF in the brain, along with 2.2 times higher cerebral levels of NGF-β, compared to female mice without knockout.Conclusions. In female mice with uPA gene knockout compared to mice without knockout, we revealed background differences and other dynamics of neurotrophin levels in the brain at melanoma growth both alone and in combination with comorbid pathology – CNP.Цель – изучить уровень нейротрофинов в головном мозге мышей с нокаутом гена урокиназы (uPA), носителей меланомы B16/F10, растущей на фоне коморбидной патологии – хронической нейрогенной боли (ХНБ).Методы и материалы. Работа выполнена на самках мышей линий С57ВL/6 (n=40) и C57BL/6-PlautmI.IBugThisPlau6FDhu/GFDhu (n=28). В основных группах моделировали ХНБ двусторонней перевязкой седалищных нервов и через 2 недели под кожу спины перевивали меланому В16/F10. Группы сравнения – ложнооперированные животные с перевивкой меланомы. Контрольные группы – ложнооперированные животные и животные с ХНБ. На 21-е сутки опухолевого роста мышей декапитировали и в головном мозге методом иммуноферментного анализа определяли содержание нейротрофического фактора мозга (BDNF); фактора роста нервов (NGF), нейротрофинов-3 (NT3) и -4 (NT4).Результаты. У мышей с нокаутом по uPA было больше NT3 (в 1,3 раза (р=0,0146)), NT4 (в 2,6 раза) и NGF-β (в 1,9 раза (р=0,0021)) и меньше BDNF (в 1,7 раза (р=0,0203)). Неспецифическим ответом головного мозга самок мышей на ХНБ и неопластический рост являлась церебральная редукция NGF-β, выраженность которой увеличивалась при сочетании патологических факторов. Бóльшая стимуляция подкожного роста меланомы у самок мышей с нокаутом uPA под влиянием ХНБ сочеталась с двухкратным уменьшением содержания NT3 и BDNF в мозге на фоне в 2,2 раза бóльшего, чем у самок без нокаута, церебрального уровня NGF-β.Заключение. У самок мышей с нокаутом гена uPA, в отличие от мышей без нокаута, выявлены фоновые отличия и иная динамика уровней нейротрофинов в головном мозге при росте меланомы в самостоятельном варианте и на фоне коморбидной патологии – ХНБ

Role of clinical and morphological data in prediction of regional lymph node involvement in early-stage breast cancer

Objective: to evaluate the frequency of axillary lymph node metastasis (detected at routine pathomorphological examination) in order to determine the group of patients who do not require lymph node surgery.Materials and methods. We analyzed medical records of 485 patients with breast cancer and no signs (either clinical or instrumental) of regional lymph node involvement who underwent surgery at the first stage of their treatment.Results and conclusion. In patients with cN0 cancer, only the size of primary tumor ≤1 cm was found to be a significant factor indicating that regional lymph nodes are not involved (in addition to palpation, ultrasound, mammography, and spiral x-ray computed tomography of the chest). In patients with сТ1c cancer, the majority of standard immunohistochemical markers cannot yet be used as predictors of regional lymph node involvement. However, tumor differentiation grade G1 is rather an exclusion, which in combination with the size of the primary tumor сТ1c can also be considered as a significant prognostic factor

Refinement of drainage-free radical mastectomy as a way to improve direct results of surgical treatment

A procedure for drainage-free radical mastectomy is proposed, which can significantly reduce not only the incidence of postoperative lymphorrhea (from 10 to 4.2%), but also its average duration (from 9 to 4 days). No postoperative wound complications accelerate healing and accordingly decrease the length of bed rest. This determines the economic benefits of the proposed procedure and makes it possible to continue combined or complex treatment