3 research outputs found
The effect of intravenous butaphosphan, B12 and C vitamins on metritis, mastitis prevalence and reproductive performance of dairy cattle
Three groups of Holstein
Friesian cows were used to test the effect of
two intravenous solutions on metritis,
mastitis prevalence and reproductive
performance. The first solution containing
3000 mg Butaphosphan (100 mg/ml) and
1.5 mg B12 Vitamin (0.05 mg/ml) was
administered to BB12 group in the first
3 days after parturition and the second
solution containing 3000 mg Butaphosphan
(100 mg/ml), 1.5 mg B12 Vitamin (0.05
mg/ml) and 7 g of C vitamin (0.1 g/ml) was
administered to BB12C group in the same
period. The control group (C) didn’t receive
any intravenous solution. The Tukey-
Krammer multiple comparison tests were
used to compare the results. The prevalence
of clinical metritis, puerperal metritis and
clinical mastitis was lower in the BB12C
group (p< 0.05), compared to BB12 and C
groups. Regarding the prevalence of metritis
and mastitis no differences was observed in
the BB12 group, compared with the C group
(p>0.05). However, no difference was
observed in the prevalence of the clinical
endometritis (p>0.05) for the all three
groups of cows. Also, the BB12C group
registered the best calving to first
insemination interval and calving to
conception interval (p< 0.05). In conclusion,
the intravenous cocktail containing
Butaphosphan, B12 and C Vitamin can
reduce the prevalence of some uterus and
udder infection in the first 7 days after
parturition in dairy cows
In vitro production of embryos at Research and Development Station for Cattle Breeding Dancu, Iasi – first bovine embryos produced in vitro in North-Eastern Romania
The in vitro production (IVP)
of bovine embryos increases the selection
intensity in cattle and reduces the generation
interval, which is very important in the
genetic gain. In Romania, this reproductive
biotechnology has shown a timid evolution
in the last years, although the need for
genetic improvement in the area is present.
The aim of this paper is to describe the work
that resulted in first bovine embryos
obtained through IVP in North-Eastern
Romania. Oocytes were collected by
slashing ovaries from slaughtered cows,
matured in a TCM199-based medium and
fertilized in TL-based medium microdrops
with sperm processed by swim-up
procedure. The presumptive embryos were
cultured one day in TCM199 and 8 days in
SOF-based medium and evaluated in days 7,
8 and 9 after fertilization. We retrieved an
average number of 83 usable oocytes/IVF
session, which represents 73.8% from the
total harvested oocytes. The average number
of cleaved embryos was 50.8 per IVF,
reflecting an average cleavage rate of
61.2%. An average of 8.6 blastocysts/IVF
session was obtained, representing 10.4% of
the selected oocytes or 16.9% of the number
of cleaved embryos. Although suboptimal,
the results were comparable with other
reports on IVP in cattle. The adapted IVP
protocol, based on maturation with
TCM199, fertilization in microdrops of TL
and culture of presumptive embryos one day
in TCM199 and afterwards in SOF seems to
offer acceptable results and will be used for
further attempts to produce bovine embryos
Cryopreservation of Bălțată cu Negru Românească cattle immature oocytes by slow freezing method: preliminary research
Research on bovine oocytes
cryopreservation is important for successful
preservation of genetically valuable animal.
The transvaginal ultrasound-guided
follicular puncture coupled with in vitro
embryo production has become competitive
and alternative method for MOET (Multiple
ovulation and embryo transfer) in dairy
cattle. The aim of this preliminary research
is to presents the result of Bălțată cu Negru
Românească (BNR) cows oocytes recovery
by two different protocols and its
cryopreservation by slow freezing method.
By applying the recovery oocytes from
slaughterhouse ovary we obtained an
average of 16.34 ± 6.71 oocytes per cow,
much higher compared with the Ovum Pick
Up (OPU) method, which reveals an
average of 2.75 ± 0.2 oocytes per cow. After
applying the slow freezing procedures using
the ethylene glycol cryoprotectant we
observed the oocytes with cumulus cells
normal with the spherical shape and normal
zone pellucida