The STACEE-32 Ground Based Gamma-ray Detector

We describe the design and performance of the Solar Tower Atmospheric Cherenkov Effect Experiment detector in its initial configuration (STACEE-32). STACEE is a new ground-based gamma ray detector using the atmospheric Cherenkov technique. In STACEE, the heliostats of a solar energy research array are used to collect and focus the Cherenkov photons produced in gamma-ray induced air showers. The large Cherenkov photon collection area of STACEE results in a gamma-ray energy threshold below that of previous detectors.Comment: 45 pages, 25 figures, Accepted for publication in Nuclear Instruments and Methods

Doing synthetic biology with photosynthetic microorganisms

The use of photosynthetic microbes as synthetic biology hosts for the sustainable production of commodity chemicals and even fuels has received increasing attention over the last decade. The number of studies published, tools implemented, and resources made available for microalgae have increased beyond expectations during the last few years. However, the tools available for genetic engineering in these organisms still lag those available for the more commonly used heterotrophic host organisms. In this mini-review, we provide an overview of the photosynthetic microbes most commonly used in synthetic biology studies, namely cyanobacteria, chlorophytes, eustigmatophytes and diatoms. We provide basic information on the techniques and tools available for each model group of organisms, we outline the state-of-the-art, and we list the synthetic biology tools that have been successfully used. We specifically focus on the latest CRISPR developments, as we believe that precision editing and advanced genetic engineering tools will be pivotal to the advancement of the field. Finally, we discuss the relative strengths and weaknesses of each group of organisms and examine the challenges that need to be overcome to achieve their synthetic biology potential.Peer reviewe

A handwritten letter from D.R. Boone regarding an enclosed check for a ticket, but writing about the lack of a defense attorney.

A handwritten letter from D.R. Boone regarding an enclosed check for a ticket, but writing about the lack of a defense attorney