In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis

The antineoplastic potential of a stable monomeric Au(II) complex with hematoporphyrin IX (Hp), namely [Au(II)Hp−2H.(H2O)2], was investigated in a panel of tumor cell lines. The complex exhibits strong cytotoxicity, whereby the leukaemia- and lymphoma-derived cell lines are more sensitive, with IC50 values comparable to those of the reference anticancer drug cisplatin. In contrast, the solid tumor models are more sensitive to the platinum drug. A comparative assessment of both agents against the human kidney cell line 293T has shown that [Au(II)Hp−2H.(H2O)2] is less cytotoxic. The gold complex induces oligonucleosomal DNA fragmentation in tumour cells following 24-hour treatment and hence its cytotoxic effect is at least partly mediated by induction of apoptotic cell death. A prominent intracellular gold accumulation was detected after treating tumor cells with [Au(II)Hp−2H.(H2O)2] which shows that its putative pharmacological targets are readily accessible after a short incubation period

D10.2 – Research Data Management Plan, version 3.0:RAGE – WP10 – D10.2

What data will be collected, processed or generated during the RAGE project? Following what methodology and standards? And what data will be shared and/or made openly available, and how will it be curated and preserved? These issues are typically described in a Data Management Plan (DMP), outlining how research data is handled during the project and after the project is completed. The RAGE DMP specifically provides guidelines on ethics, data protection and open research data access to RAGE researchers involved in WP5 (Case experiments) and WP8 (Validation). Ethics and data protection are especially relevant in view of the games and audiences targeted by RAGE. Therefore, RAGE is one of the participating projects in the EU open research data pilot, an initiative under Horizon 2020 that aims at improving and maximising the access to and re-use of research data created in European projects.This document is the third and final iteration of the DMP