Investigation on the Interaction Between 111 In3+ and DTPA in Water by Electromigration Analysis

Gamma radionuclide 111In is often used in radiodiagnostics due to his suitable characteristics. Its typical clinical form is as a chelate of some polyaminopolycarboxylic acids, in most cases - DTPA. Therefore, it is of a practical interest to understand the chemical stability of the complex In-DTPA, as well as the Indium-DTPA equilibrium system kinetic. In this paper some results of investigations of behaviour of 111 In(III) in aqueous solutions in the presence of DTPA carried out by the method of horizontal zone electrophoresis in a free electrolyte are presented. Some thermodynamic parameters have been estimated as follows: effective charge of the complex In-DTPA zeff. = -1,7; stability constant β= (1,5 ±0,3). 10+29; kinetic constants for reactions of formation (k1) and degradation (k2) of chelate: lgk1 = -1,0 and lgk2 = -2,6

TM-167: An Electrophoretic Investigation of Its Properties in Ultradiluted Aqueous Solutions

The gamma-radionuclide Tm-167 is often used in the clinical radiodiagnostics and nuclear medicine. The usual form of its application is as a citrate complex. Nevertheless, there are considerable amonunts of the simple hydrated Thulim (III) species that can be expected in the blood and body tissue in some cases. Main reasons for that fact are metabolic and hydrolytic processes as well as failures in radiopharmaceutical preparation (kit engagement). Therefore, it is of a practical interest to know chemical properties as well as the behavior of the Tmaq.3+ in water solutions at microconcentrations as low as 10-7 - 10-8 mol.l-1. This will help understanding how to prevent an undesired side effect, especially for in-vivo treatment with radioactive Thulium drugs. In this radioanalytical data, including values of parameters such as ion mobilities, molar volumes, Stocks radii, hydration numbers and diffusion coefficients in water solutions is presented

Effect of Complexes of Cobalt With Aminoacids on the Replication of Herpes Simplex Virus Type 1 (HSV-1)

Cobalt, being essential metal, influences different physiological and enzymatic functions. As cobalt does not accumulate in the body, Co-compounds have relatively low toxicity. The aim of the present study is the effect of complexes of Co(II) with aminoacids - lysine, arginine, histidine and serine on HSV-1 replication. No effect of [O2Co(his)4].nH2O and [O2Co(arg)2].nH2O on HSV-1 infection in vitro was found. Both, [O2Co(lys)2].nH2O and [O2Co(ser)2].nH2O suppress the attachement of HSV-1 particles onto target cells and the viral replication as well. Moreover, the properties of the particular Co-complex (charge, stability, structure) are manifestated by their virucidal effect. Thus, [O2Co(ser)2].nH2O irreversibly inhibits the infectious activity of free HSV-1 virions, while virucidal effect of [O2Co(lys)2].nH2O is completely reversible after the 2h of contact

Effect of Complexes of Zinc, Cobalt and Copper With D-Aminosugars on the Replication of Herpes Simplex Virus Type 1 (HSV-1)

Our previous results show that Zn(pic)2 and Zn(asp)2 inhibit key steps of the replication of HSV-1. Anti-HSV effect of complexes of Co(II) with aminoacids Lys and Ser was also found. In the present study we describe the effect of complexes of Zn(II), Co(II) and Cu(II) with D-aminosugars on the replication of HSV-1 and on the infectivity of free virions. The experiments were done using primary rabbit kidney cells (r.k.), diploid human embryonal fibroblasts (F) and Vero cells. No differences in the toxicity of metal complexes on diploid cells- r.k. and F, were found. Neither metal complexes, nor ligands-galactosoxime and glucosoxime, influenced the viral replication. During 1-4h prolonged contact only Cu(Gl.NOH)2 inactivated HSV-1 virions up to 90%. The results show that D-aminosugars are not suitable ligands for Zn(II), Cu(II) and Co(II) in respect of the inhibition of viral replication. However, only Cu(Gl.NOH)2 was able to inhibit the infectivity of free virions

Complexes of Zinc With Picolinic and Aspartic Acids Inactivate Free Varicella-Zoster Virions

Zn(II) picolinate and aspartate, Zn(pic)2 and Zn(asp)2, have been shown to inhibit key steps of the replication of HSV-1. In the present study we describe the effect of Zn(pic)2 and Zn(asp)2 on the replication of VZV and on the infectivity of free virions. The experiments are done using BHK-21 cells, a clinical isolate of VZV and Zn-complexes in concentration of 10 μM. When Zn-complexes are present during the whole period of infection, the yield of infectious virus progeny decreases up to 98%. The infectivity of VZV is completely restored after the removal of zinc. The virucidal effect is manifested at the 2nd h of contact, when 90% of the virions are inactivated. The results show that both Zn(pic)2 and Zn(asp)2 specifically inactivate free VZV virions with no effect on viral replication