Mite Diet Sequences Obtained by High Throughput Sequencing of Gut Contents of Freshly Collected Water Mites

Cytochrome oxidase subunit I (COI) barcode sequences in this file were obtained from gut DNA extracted from 54 freshly collected water mites, comprising 21 Lebertia quinquemaculosa , 30 Lebertia davidcooki , 1 Limnesia , and 2 Arrenurus specimens. Methods and other details about these sequences are described in a paper by the same authors in a submitted publication (2021: URL to be given here when published). Data on collection locations, primers (mLep and LCOI), amino acid translations, etc. are included in corresponding sequences uploaded to GenBank. The right column below contains additional notes on naming the taxa of the sequences that were not included in the GenBank annotation. These notes include the highest percentage identity to a previous sequence in GenBank as determined by BLASTN in June 2018. The FASTA file name given here includes the Accession ID, followed by the best match taxon (at an appropriate taxonomic level, dependent on the percent identity, as described in the notes in the right-hand column), the phrase water mite diet isolate , a specific RamLab sequence identifier of the sequence, and then the COI gene description. Accession IDs of sequences uploaded to GenBank begin with MW; other sequences begin with RL and a RamLab sequence identifier. The RamLab sequence identifier in the FASTA name includes information as follows: RamLab ID number-location and date of collection with three location letters (e.g., BHL stands for Blue Heron Lagoon) and the date usually in a 6-character format of MMDDYY-information on the location of sequence on the Illunina sequencing plate-and a 4- to 6-character identifier of the mite species (Lq=L. quinquemaculosa ; Ldc=L. davidcooki ; Lim=Limnesia ; Arr=Arrenurus ) and the animal number in that series of experiments (2 digits)

The biodiversity of freshwater Crustaceans revealed by taxonomy and mitochondrial DNA barcodes

Cytochrome oxidase subunit I (COI) barcode sequences in this file were obtained from specimens collected by plankton net in western Lake Erie in 2012 & 2013, along with later specimens collected at various locations and times, including some collected in Belize in 2015. Methods and other details about these sequences are described in a paper by the same authors in a submitted publication (2021: URL to be given here when published). The right columns below contain additional notes on lengths of sequences, GenBank accession ID (when obtained), and annotation as to whether the sequence represents a new barcode for its genus or species taxon. According to our experience, a DNA identity of \u3e96.5% with previous GenBank barcodes is a reliable range for determining a species level barcode for that morpho species; a DNA identity of 90.5% to 96.5% with previous barcodes is sufficient to identify genus. DNA identities within these ranges are considered to be barcode confirmations. Conversely, DNA identities outside of these ranges are considered to be new barcodes for that species or genus, respectively. Contradictions with previous GenBank sequences are discussed in the manuscript. The submitted manuscript includes the highest percentage identity to a previous sequence in GenBank as determined by BLASTN in June2021. The FASTA file name given here begins with a Ram Lab ID number-location and date of collection with format varying somewhat between various collections/collectors but generally including several (usually three) location letters (e.g., BHL stands for Blue Heron Lagoon) and the date usually in a 6-character format of MMDDYY, and optionally a sample number for that date either preceding the location letters or following the date. Collection location abbreviations include the following: All sequences starting with PM, Toledo Harbor in western Lake Erie; LMUSK, Lake Muskoday, Belle Isle, Detroit; SCL, Saint Clair River; BHL, Blue Heron Lagoon, Belle Isle; LE, LakeErie; LSC, Lake St.Clair; MMLE; Metzgers Marsh, LakeErie; MM, Metzgers Marsh; LP, Leonard Preserve, Manchester, Michigan; HR, Huron River Drive, Ypsilanti, Michigan; LCL, Little Cedar Lake, Orion, MI; HLE, Harbor Lake Erie; LHLE, Lorain Harbor Lake Erie; BZEB1P, Cenote in Shipstern Reserve, Corozal, Belize, Central America

Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content

Chironomids are one of the most biodiverse and abundant members of freshwater ecosystems. They are a food source for many organisms, including fish and water mites. The accurate identification of chironomids is essential for many applications in ecological research, including determining which chironomid species are present in the diets of diverse predators. Larval and adult chironomids from diverse habitats, including lakes, rivers, inland gardens, coastal vegetation, and nearshore habitats of the Great Lakes, were collected from 2012 to 2019. After morphological identification of chironomids, DNA was extracted and cytochrome oxidase I (COI) barcodes were PCR amplified and sequenced. Here we describe an analysis of biodiverse adult and larval chironomids in the Great Lakes region of North America based on new collections to improve chironomid identification by curating a chironomid DNA barcode database, thereby expanding the diversity and taxonomic specificity of DNA reference libraries for the Chironomidae family. In addition to reporting many novel chironomid DNA barcodes, we demonstrate here the use of this chironomid COI barcode database to improve the identification of DNA barcodes of prey in the liquefied diets of water mites. The species identifications of the COI barcodes of chironomids ingested by Lebertia davidcooki and L. quinquemaculosa are more diverse for L. davidcooki and include Parachironomus abortivus, Cryptochironomus ponderosus. Parachironomus tenuicaudatus, Glyptotendipes senilis, Dicrotendipes modestus, Chironomus riparius, Chironomus entis/plumosus, Chironomus maturus, Chironomus crassicaudatus, Endochironomus subtendens, Cricotopus sylvestris, Cricotopus festivellus, Orthocladius obumbratus, Tanypus punctipennis, Rheotanytarsus exiguus gr., and Paratanytarsus nr. bituberculatus