Safe surgery – analysis of the implementation of the checklist by Swot matrix / Cirurgia segura – análise da implementação da lista de verificação através da matriz SWOT

Objetivos: Conhecer o processo de implementação da lista de verificação de cirurgia segura e Analisar o processo de implementação da lista de verificação de cirurgia segura em centro cirúrgico de um hospital federal, através da matriz SWOT. Métodos: Trata-se de um estudo descritivo e exploratório, com abordagem quanti-qualitativa. Os dados foram obtidos através de um questionário, com amostra de 56 participantes. O cenário foi o centro cirúrgico de um Hospital Federal situado no Município do Rio de Janeiro. Resultados: O processo de implementação da lista ainda não está implantado de forma sistemática e consistente; constatou-se a necessidade de implementação da lista em todos os procedimentos cirúrgicos, onde destaca-se as responsabilidades dos líderes e gestores na adoção de processo para cirurgia segura. Considerações finais: Foi evidenciada a importância atribuída à implementação da lista de verificação de cirurgia segura, considerada como estratégia para melhoria da segurança do paciente e minimização de erros

Safe surgery – analysis of the implementation of the checklist by Swot matrix / Cirurgia segura – análise da implementação da lista de verificação através da matriz SWOT

Objetivos: Conhecer o processo de implementação da lista de verificação de cirurgia segura e Analisar o processo de implementação da lista de verificação de cirurgia segura em centro cirúrgico de um hospital federal, através da matriz SWOT. Métodos: Trata-se de um estudo descritivo e exploratório, com abordagem quanti-qualitativa. Os dados foram obtidos através de um questionário, com amostra de 56 participantes. O cenário foi o centro cirúrgico de um Hospital Federal situado no Município do Rio de Janeiro. Resultados: O processo de implementação da lista ainda não está implantado de forma sistemática e consistente; constatou-se a necessidade de implementação da lista em todos os procedimentos cirúrgicos, onde destaca-se as responsabilidades dos líderes e gestores na adoção de processo para cirurgia segura. Considerações finais: Foi evidenciada a importância atribuída à implementação da lista de verificação de cirurgia segura, considerada como estratégia para melhoria da segurança do paciente e minimização de erros

Current Knowledge and Priorities for Future Research in Late Effects after Hematopoietic Cell Transplantation for Inherited Bone Marrow Failure Syndromes: Consensus Statement from the Second Pediatric Blood and Marrow Transplant Consortium International Conference on Late Effects after Pediatric Hematopoietic Cell Transplantation

Transplantation and immunomodulatio

Late Effects Screening Guidelines after Hematopoietic Cell Transplantation for Inherited Bone Marrow Failure Syndromes: Consensus Statement From the Second Pediatric Blood and Marrow Transplant Consortium International Conference on Late Effects After Pediatric HCT

Transplantation and immunomodulatio

The Porphyromonas gingivalis/host interactome shows enrichment in GWASdb genes related to Alzheimer’s disease, diabetes and cardiovascular diseases

Periodontal disease is of established aetiology in which polymicrobial synergistic ecology has become dysbiotic under the influence of Porphyromonas gingivalis. Following breakdown of the host’s protective oral tissue barriers, P. gingivalis migrates to developing inflammatory pathologies that associate with Alzheimer’s disease (AD). Periodontal disease is a risk factor for cardiovascular disorders (CVD), type II diabetes mellitus (T2DM), AD and other chronic diseases, whilst T2DM exacerbates periodontitis. This study analysed the relationship between the P. gingivalis/host interactome and the genes identified in genome-wide association studies (GWAS) for the aforementioned conditions using data from GWASdb (P<1E-03) and, in some cases, from the NCBI/EBI GWAS database (P< 1E-05). Gene expression data from periodontitis or P. gingivalis microarray was compared to microarray datasets from the AD hippocampus and/or from carotid artery plaques. The results demonstrated that the host genes of the P. gingivalis interactome were significantly enriched in genes deposited in GWASdb genes related to cognitive disorders, AD and dementia, and its co-morbid conditions T2DM, obesity, and CVD. The P. gingivalis/host interactome was also enriched in GWAS genes from the more stringent NCBI-EBI database for AD, atherosclerosis and T2DM. The misregulated genes in periodontitis tissue or P. gingivalis infected macrophages also matched those in the AD hippocampus or atherosclerotic plaques. Together, these data suggest important gene/environment interactions between P. gingivalis and susceptibility genes or gene expression changes in conditions where periodontal disease is a contributory factor

Mucopolysaccharidosis I, II, and VI: Brief review and guidelines for treatment

Mucopolysaccharidoses (MPS) are rare genetic diseases caused by the deficiency of one of the lysosomal enzymes involved in the glycosaminoglycan (GAG) breakdown pathway. This metabolic block leads to the accumulation of GAG in various organs and tissues of the affected patients, resulting in a multisystemic clinical picture, sometimes including cognitive impairment. Until the beginning of the XXI century, treatment was mainly supportive. Bone marrow transplantation improved the natural course of the disease in some types of MPS, but the morbidity and mortality restricted its use to selected cases. The identification of the genes involved, the new molecular biology tools and the availability of animal models made it possible to develop specific enzyme replacement therapies (ERT) for these diseases. At present, a great number of Brazilian medical centers from all regions of the country have experience with ERT for MPS I, II, and VI, acquired not only through patient treatment but also in clinical trials. Taking the three types of MPS together, over 200 patients have been treated with ERT in our country. This document summarizes the experience of the professionals involved, along with the data available in the international literature, bringing together and harmonizing the information available on the management of these severe and progressive diseases, thus disclosing new prospects for Brazilian patients affected by these conditions

TLR9 activation dampens the early inflammatory response to paracoccidioides brasiliensis, Impacting host survival

Background: Paracoccidioides brasiliensis causes paracoccidioidomycosis, one of the most prevalent systemic mycosis in Latin America. Thus, understanding the characteristics of the protective immune response to P. brasiliensis is of interest, as it may reveal targets for disease control. The initiation of the immune response relies on the activation of pattern recognition receptors, among which are TLRs. Both TLR2 and TLR4 have been implicated in the recognition of P. brasiliensis and regulation of the immune response. However, the role of TLR9 during the infection by this fungus remains unclear.J.F. Menino was supported by a grant from Fundacao para a Ciencia e Tecnologia (FCT), Portugal (SFRH/BD/33446/2008). This work was supported by a grant from FCT (PTDC/BIA-MIC/108309/2008). M. Saraiva is a Ciencia 2007 fellow and M. Sturme is a Ciencia 2008 fellow. We would also like to thank FAPESP (Fundacao para Amparo a Pesquisa do Estado de Sao Paulo) and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript