Lung and Heart Biology of the Dp16 Mouse Model of down Syndrome: Implications for Studying Cardiopulmonary Disease

(1) Background: We sought to investigate the baseline lung and heart biology of the Dp16 mouse model of Down syndrome (DS) as a prelude to the investigation of recurrent respiratory tract infection. (2) Methods: In controls vs. Dp16 mice, we compared peripheral blood cell and plasma analytes. We examined baseline gene expression in lungs and hearts for key parameters related to susceptibility of lung infection. We investigated lung and heart protein expression and performed lung morphometry. Finally, and for the first time each in a model of DS, we performed pulmonary function testing and a hemodynamic assessment of cardiac function. (3) Results: Dp16 mice circulate unique blood plasma cytokines and chemokines. Dp16 mouse lungs over-express the mRNA of triplicated genes, but not necessarily corresponding proteins. We found a sex-specific decrease in the protein expression of interferon α receptors, yet an increased signal transducer and activator of transcription (STAT)-3 and phospho-STAT3. Platelet-activating factor receptor protein was not elevated in Dp16 mice. The lungs of Dp16 mice showed increased stiffness and mean linear intercept and contained bronchus-associated lymphoid tissue. The heart ventricles of Dp16 mice displayed hypotonicity. Finally, Dp16 mice required more ketamine to achieve an anesthetized state. (4) Conclusions: The Dp16 mouse model of DS displays key aspects of lung heart biology akin to people with DS. As such, it has the potential to be an extremely valuable model of recurrent severe respiratory tract infection in DS

Near-infrared electroluminescence from conjugated polymer/lanthanide porphyrin blends

Near-infrared-emitting polymer light-emitting diodes (PLEDs) have been fabricated using blends of conjugated polymers and lanthanide tetraphenylporphyrin complexes. Host polymers include MEH–PPV and a bis-alkoxy-substituted poly(p-phenylene) (PPP–OR11), and the lanthanide complexes include Yb(TPP)acac and Er(TPP)acac (where TPP = 5,10,15,20-tetraphenylporphyrin and acac = acetylacetonate). Electroluminescence (EL) is observed at 977 nm from devices fabricated using MEH–PPV or PPP–OR11 blended with Yb(TPP)acac, and EL is observed at 1560 nm from a device fabricated using a blend of MEH–PPV and Er(TPP)acac. Visible EL from the host polymers is strongly suppressed in all of the devices, however, in the device fabricated using the PPP–OR11 polymer blue emission from the host is completely quenched. Very efficient quenching of the EL from the host in the PPP–OR11 device is believed to occur due to efficient Förster energy transfer, which is facilitated by the excellent spectral overlap between the PPP–OR11 fluorescence and the Soret absorption band of the TPP ligand

Near-infrared electroluminescence from conjugated polymer/lanthanide porphyrin blends

Near-infrared-emitting polymer light-emitting diodes (PLEDs) have been fabricated using blends of conjugated polymers and lanthanide tetraphenylporphyrin complexes. Host polymers include MEH-PPV and a bis-alkoxy-substituted poly(p-phenylene) (PPP–OR11), and the lanthanide complexes include Yb(TPP)acac and Er(TPP)acac (where TPP = 5,10,15,20-tetraphenylporphyrin and acac=acetylacetonate). Electroluminescence (EL) is observed at 977 nm from devices fabricated using MEH-PPV or PPP–OR11 blended with Yb(TPP)acac, and EL is observed at 1560 nm from a device fabricated using a blend of MEH-PPV and Er(TPP)acac. Visible EL from the host polymers is strongly suppressed in all of the devices, however, in the device fabricated using the PPP-OR11 polymer blue emission from the host is completely quenched. Very efficient quenching of the EL from the host in the PPP-OR11 device is believed to occur due to efficient Förster energy transfer, which is facilitated by the excellent spectral overlap between the PPP-OR11 fluorescence and the Soret absorption band of the TPP ligand