16 research outputs found
Mechanical properties of composite scaffolds from poly(3-hydroxybutyrate) and sodium alginate
Controlled release profiles of dipyridamole from biodegradable microspheres on the base of poly(3-hydroxybutyrate).
Evaluation of eliciting activity of peptidil prolyl cys/trans isomerase from <em>Pseudonomas fluorescens</em> encapsulated in sodium alginate regarding plant resistance to viral and fungal pahogens
Use of chemical pesticides poses a threat for environment and human health, so green technologies of crop protection are of high demand. Some microbial proteins able to activate plant defense mechanisms and prevent the development of resistance in plant pathogens, may be good alternative to chemicals, but practical use of such elicitors is limited due to need to protect them against adverse environment prior their delivery to target receptors of plant cells. In this study we examined a possibility to encapsulate heat resistant FKBP-type peptidyl prolyl cis-trans isomerase (PPIase) from Pseudomonas fluorescens, which possesses a significant eliciting activity in relation to a range of plant pathogens, in sodium alginate microparticles and evaluated the stability of resulted complex under long-term UV irradiation and in the presence of proteinase K, as well as its eliciting activity in three different βplant-pathogenβ models comparing to that of free PPIase. The obtained PPIase-containing microparticles consisted of 70% of sodium alginate, 20% of bovine serum albumin, and 10% of PPIase. In contrast to free PPIase, which lost its eliciting properties after 8-h UV treatment, encapsulated PPIase kept its eliciting ability unchanged; after being exposed to proteinase K, its eliciting ability twice exceeded that of free PPIase. Using βtobacco-TMVβ, βtobacco-Alternaria longipesβ, and βwheat-Stagonospora nodorumβ model systems, we showed that encapsulation process did not influence on the eliciting activity of PPIase. In the case of the βwheat-S. nodorumβ model system, we also observed a significant eliciting activity of alginate-albumin complex and almost doubled activity of encapsulated PPIase as compared to the free PPIase. As far as we know, this is the first observation of a synergistic interaction between alginate and other compound possessing any bioactive properties. The results of the study show some prospects for a PPIase use in agriculture
Biosynthesis of Alginate and Poly(3-Hydroxybutyrate) by the Bacterial Strain Azotobacter agile 12
Citrate Cycle Intermediates in the Metabolism of Aspartate and Lactate by Propionibacterium freudenreichii
Interactions between Pyruvate and Lactate Metabolism in Propionibacterium freudenreichii
Π€ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠΈΠΊΡΠΎΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΠΏΠ»Π΅Π½ΠΎΠΊ ΠΏΠΎΠ»ΠΈ-3-ΠΎΠΊΡΠΈΠ±ΡΡΠΈΡΠ°ΡΠ° Ρ ΡΠ΅Π³ΡΠ»ΠΈΡΡΠ΅ΠΌΠΎΠΉ ΡΠΎΠΏΠΎΠ³ΡΠ°ΡΠΈΠ΅ΠΉ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠΈ
The possibility of fabrication of microstructured poly-3-hydroxybutyrate films by self-assembly water microdroplets technique, using artificial templates and polymer inverse emulsions has been studied. It has been established that self-assembly water microdroplets technique allows forming ordered microstructures of poly-3-hydroxybutyrate with a hexagonal arrangement of cells with an adjustable diameter from 1 to 4 ΡΡ. It has been shown that application of inverse emulsions of poly-3-hydroxybutyrate allows us to fabricate porous films with a pore size in the range from 0.4 to 3 ^m, while the structure of the films and the pore size can be controlled by changing the polymer concentration in the dispersion medium and the volume ratio of the phases. Using spin-coating technique and artificial templates, it is possible to obtain poly-3-hydroxybutyrate microstructured replicas, which are characterized by a high degree of uniformity and the absence of defective areas. It has been shown that the formed microstructured poly-3-hydroxybutyrate films with controlled surface topography are promising for use as scaffolds for stem cells.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π° Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΈΠΊΡΠΎΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΠ»Π΅Π½ΠΎΠΊ ΠΏΠΎΠ»ΠΈ-3-ΠΎΠΊΡΠΈΠ±ΡΡΠΈΡΠ°ΡΠ° ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Β«ΡΠ°ΠΌΠΎΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈΒ» ΠΌΠΈΠΊΡΠΎΠΊΠ°ΠΏΠ΅Π»Ρ Π²ΠΎΠ΄Ρ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠ°Π±Π»ΠΎΠ½ΠΎΠ² ΠΈ ΠΎΠ±ΡΠ°ΡΠ½ΡΡ
ΡΠΌΡΠ»ΡΡΠΈΠΉ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Β«ΡΠ°ΠΌΠΎΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈΒ» ΠΌΠΎΠΆΠ½ΠΎ ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°ΡΡ ΡΠΏΠΎΡΡΠ΄ΠΎΡΠ΅Π½Π½ΡΠ΅ ΠΌΠΈΠΊΡΠΎΡΡΡΡΠΊΡΡΡΡ ΠΏΠΎΠ»ΠΈ-3-ΠΎΠΊΡΠΈΠ±ΡΡΠΈΡΠ°ΡΠ° Ρ Π³Π΅ΠΊΡΠ°Π³ΠΎΠ½Π°Π»ΡΠ½ΡΠΌ ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΠ΅Π΅ΠΊ ΡΠ΅Π³ΡΠ»ΠΈΡΡΠ΅ΠΌΠΎΠ³ΠΎ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠ° ΠΎΡ 1 Π΄ΠΎ 4 ΠΌΠΊΠΌ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΡΠ΅ΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΎΠ±ΡΠ°ΡΠ½ΡΡ
ΡΠΌΡΠ»ΡΡΠΈΠΉ ΠΏΠΎΠ»ΠΈ-3-ΠΎΠΊΡΠΈΠ±ΡΡΠΈΡΠ°ΡΠ° ΠΌΠΎΠΆΠ½ΠΎ ΠΏΠΎΠ»ΡΡΠΈΡΡ ΠΏΠΎΡΠΈΡΡΡΠ΅ ΠΏΠ»Π΅Π½ΠΊΠΈ Ρ Π·Π°Π΄Π°Π½Π½ΡΠΌ ΡΠ°Π·ΠΌΠ΅ΡΠΎΠΌ ΠΏΠΎΡ ΠΎΡ 0,4 Π΄ΠΎ 3 ΠΌΠΊΠΌ, ΠΏΡΠΈ ΡΡΠΎΠΌ ΡΡΡΡΠΊΡΡΡΡ ΠΏΠ»Π΅Π½ΠΎΠΊ ΠΈ ΡΠ°Π·ΠΌΠ΅Ρ ΠΏΠΎΡ Π² Π½ΠΈΡ
ΠΌΠΎΠΆΠ½ΠΎ ΡΠ΅Π³ΡΠ»ΠΈΡΠΎΠ²Π°ΡΡ ΠΏΡΡΠ΅ΠΌ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ° Π² Π΄ΠΈΡΠΏΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΡΠ΅Π΄Π΅ ΠΈ ΠΎΠ±ΡΠ΅ΠΌΠ½ΠΎΠ³ΠΎ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΡΠ°Π·. Π‘ ΠΏΠΎΠΌΠΎΡΡΡ ΠΌΠ΅ΡΠΎΠ΄Π° ΡΠ΅Π½ΡΡΠΈΡΡΠ³ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠ°Π±Π»ΠΎΠ½ΠΎΠ² ΠΌΠΎΠΆΠ½ΠΎ ΡΠΎΠ·Π΄Π°Π²Π°ΡΡ ΡΠΎΡΠ½ΡΠ΅ ΡΠ΅ΠΏΠ»ΠΈΠΊΠΈ ΠΏΠΎΠ»ΠΈ-3-ΠΎΠΊΡΠΈΠ±ΡΡΠΈΡΠ°ΡΠ°, ΠΊΠΎΡΠΎΡΡΠ΅ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΡΡ Π²ΡΡΠΎΠΊΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ ΠΎΠ΄Π½ΠΎΡΠΎΠ΄Π½ΠΎΡΡΠΈ ΠΏΠΎ Π²ΡΠ΅ΠΉ ΠΏΠ»ΠΎΡΠ°Π΄ΠΈ ΠΈ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠ½ΡΡ
ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΌΠΈΠΊΡΠΎΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΏΠ»Π΅Π½ΠΊΠΈ ΠΏΠΎΠ»ΠΈ-3-ΠΎΠΊΡΠΈΠ±ΡΡΠΈΡΠ°ΡΠ° Ρ ΡΠ΅Π³ΡΠ»ΠΈΡΡΠ΅ΠΌΠΎΠΉ ΡΠΎΠΏΠΎΠ³ΡΠ°ΡΠΈΠ΅ΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½Ρ Π΄Π»Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠΊΠ°ΡΡΠΎΠ»Π΄ΠΎΠ² Π΄Π»Ρ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠ²ΠΎΠ»ΠΎΠ²ΡΡ
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