Low infra red laser light irradiation on cultured neural cells: effects on mitochondria and cell viability after oxidative stress

<p>Abstract</p> <p>Background</p> <p>Considerable interest has been aroused in recent years by the well-known notion that biological systems are sensitive to visible light. With clinical applications of visible radiation in the far-red to near-infrared region of the spectrum in mind, we explored the effect of coherent red light irradiation with extremely low energy transfer on a neural cell line derived from rat pheochromocytoma. We focused on the effect of pulsed light laser irradiation vis-à-vis two distinct biological effects: neurite elongation under NGF stimulus on laminin-collagen substrate and cell viability during oxidative stress.</p> <p>Methods</p> <p>We used a 670 nm laser, with extremely low peak power output (3 mW/cm²) and at an extremely low dose (0.45 mJ/cm²). Neurite elongation was measured over three days in culture. The effect of coherent red light irradiation on cell reaction to oxidative stress was evaluated through live-recording of mitochondria membrane potential (MMP) using JC1 vital dye and laser-confocal microscopy, in the absence (photo bleaching) and in the presence (oxidative stress) of H₂O₂, and by means of the MTT cell viability assay.</p> <p>Results</p> <p>We found that laser irradiation stimulates NGF-induced neurite elongation on a laminin-collagen coated substrate and protects PC12 cells against oxidative stress.</p> <p>Conclusion</p> <p>These data suggest that red light radiation protects the viability of cell culture in case of oxidative stress, as indicated by MMP measurement and MTT assay. It also stimulates neurite outgrowth, and this effect could also have positive implications for axonal protection.</p

A study of CP violation in the decays B±→[K+K-π+π-]Dh± (h= K, π) and B±→[π+π-π+π-]Dh±

The first study of CP violation in the decay mode B±→[K+K-π+π-]Dh± , with h= K, π , is presented, exploiting a data sample of proton–proton collisions collected by the LHCb experiment that corresponds to an integrated luminosity of 9 \,fb - 1 . The analysis is performed in bins of phase space, which are optimised for sensitivity to local CP asymmetries. CP -violating observables that are sensitive to the angle γ of the Unitarity Triangle are determined. The analysis requires external information on charm-decay parameters, which are currently taken from an amplitude analysis of LHCb data, but can be updated in the future when direct measurements become available. Measurements are also performed of phase-space integrated observables for B±→[K+K-π+π-]Dh± and B±→[π+π-π+π-]Dh± decays

Measurement of τL using the Bs0 →J/ψη decay mode

Using a proton–proton collision data sample collected by the LHCb detector and corresponding to an integrated luminosity of 5.7fb-1 , the lifetime of the light Bs0 mass eigenstate, τL , is measured using the Bs0→J/ψη decay mode to be τL=1.445±0.016(stat)±0.008(syst)ps. A combination of this result with a previous LHCb analysis using an independent dataset corresponding to 3 fb - 1 of integrated luminosity gives τL=1.452±0.014±0.007±0.002ps, where the first uncertainty is statistical, the second due to the uncorrelated part of the systematic uncertainty and the third due to the correlated part of the systematic uncertainty

Amplitude analysis of the Λb0→pK−γ decay

The resonant structure of the radiative decay Λb0→pK−γ in the region of proton-kaon invariant-mass up to 2.5 GeV/c2 is studied using proton-proton collision data recorded at centre-of-mass energies of 7, 8, and 13 TeV collected with the LHCb detector, corresponding to a total integrated luminosity of 9 fb−1. Results are given in terms of fit and interference fractions between the different components contributing to this final state. Only Λ resonances decaying to pK− are found to be relevant, where the largest contributions stem from the Λ(1520), Λ(1600), Λ(1800), and Λ(1890) states

Open charm production and asymmetry in ppNe collisions at sNN=\sqrt{s_{\scriptscriptstyle\rm NN}} = 68.5 GeV

A measurement of $D^0$ meson production by the LHCb experiment in its fixed-target configuration is presented. The production of $D^0$ mesons is studied with a beam of 2.5 TeV protons colliding on a gaseous neon target at rest, corresponding to a nucleon-nucleon centre-of-mass energy of $\sqrt{s_{\rm NN}}$ = 68.5 GeV. The $D^0$ and ${\overline D^0}$ production cross-section in $p$Ne collisions in the centre-of-mass rapidity range $y^{\star}\in [-2.29, 0]$ is found to be $\sigma_{D^{0}}^{y^\star \in [-2.29, 0]} = 48.2 \pm 0.3 \pm 4.5 \,\mu\textrm{b/nucleon}$ where the first uncertainty is statistical and the second is systematic. The $D^0-{\overline D^0}$ production asymmetry is also evaluated and suggests a negative trend at large negative $y^{\star}$. The considered models do not account precisely for all the features observed in the LHCb data, but theoretical predictions including 1$\%$ intrinsic charm and 10$\%$ recombination contributions better describe the data than the other models considered.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-015.html (LHCb public pages). arXiv admin note: text overlap with arXiv:1810.0790

Charmonium production in pNe collisions at √sNN=68.5 GeV

The measurement of charmonium states produced in proton-neon (pNe) collisions by the LHCb experiment in its fixed-target configuration is presented. The production of J/ψ and ψ(2 S) mesons is studied with a beam of 2.5 TeV protons colliding on gaseous neon targets at rest, corresponding to a nucleon-nucleon centre-of-mass energy sNN=68.5GeV . The data sample corresponds to an integrated luminosity of 21.7 ± 1.4 nb - 1 . The J/ψ and ψ(2 S) hadrons are reconstructed in μ+μ- final states. The J/ψ production cross-section per target nucleon in the centre-of-mass rapidity range y⋆∈ [- 2.29 , 0] is found to be 506±8±46nb/nucleon . The ratio of J/ψ and D cross-sections is evaluated to (1.06 ± 0.02 ± 0.09) % . The ψ(2 S) to J/ψ relative production rate is found to be (1.67 ± 0.27 ± 0.10) % in good agreement with other measurements involving beam and target nuclei of similar sizes

Observation of Cabibbo-suppressed two-body hadronic decays and precision mass measurement of the Ωc0\Omega_{c}^{0} baryon

The first observation of the singly Cabibbo-suppressed $\Omega_{c}^{0}\to\Omega^{-}K^{+}$ and $\Omega_{c}^{0}\to\Xi^{-}\pi^{+}$ decays is reported, using proton-proton collision data at a centre-of-mass energy of $13\,{\rm TeV}$, corresponding to an integrated luminosity of $5.4\,{\rm fb}^{-1}$, collected with the LHCb detector between 2016 and 2018. The branching fraction ratios are measured to be $\frac{\mathcal{B}(\Omega_{c}^{0}\to\Omega^{-}K^{+})}{\mathcal{B}(\Omega_{c}^{0}\to\Omega^{-}\pi^{+})}=0.0608\pm0.0051({\rm stat})\pm 0.0040({\rm syst})$, $\frac{\mathcal{B}(\Omega_{c}^{0}\to\Xi^{-}\pi^{+})}{\mathcal{B}(\Omega_{c}^{0}\to\Omega^{-}\pi^{+})}=0.1581\pm0.0087({\rm stat})\pm0.0043({\rm syst})\pm0.0016({\rm ext})$. In addition, using the $\Omega_{c}^{0}\to\Omega^{-}\pi^{+}$ decay channel, the $\Omega_{c}^{0}$ baryon mass is measured to be $M(\Omega_{c}^{0})=2695.28\pm0.07({\rm stat})\pm0.27({\rm syst})\pm0.30({\rm ext})\,{\rm MeV}/c^{2}$, improving the precision of the previous world average by a factor of four.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2023-011.html (LHCb public pages

Studies of η\eta and η′\eta' production in pppp and ppPb collisions

The production of $\eta$ and $\eta'$ mesons is studied in proton-proton and proton-lead collisions collected with the LHCb detector. Proton-proton collisions are studied at center-of-mass energies of $5.02$ and $13~{\rm TeV}$, and proton-lead collisions are studied at a center-of-mass energy per nucleon of $8.16~{\rm TeV}$. The studies are performed in center-of-mass rapidity regions $2.5<y_{\rm c.m.}<3.5$ (forward rapidity) and $-4.0<y_{\rm c.m.}<-3.0$ (backward rapidity) defined relative to the proton beam direction. The $\eta$ and $\eta'$ production cross sections are measured differentially as a function of transverse momentum for $1.5<p_{\rm T}<10~{\rm GeV}$ and $3<p_{\rm T}<10~{\rm GeV}$, respectively. The differential cross sections are used to calculate nuclear modification factors. The nuclear modification factors for $\eta$ and $\eta'$ mesons agree at both forward and backward rapidity, showing no significant evidence of mass dependence. The differential cross sections of $\eta$ mesons are also used to calculate $\eta/\pi^0$ cross section ratios, which show evidence of a deviation from the world average. These studies offer new constraints on mass-dependent nuclear effects in heavy-ion collisions, as well as $\eta$ and $\eta'$ meson fragmentation.Comment: All figures and tables, along with machine-readable versions and any supplementary material and additional information, are available at https://lhcbproject.web.cern.ch/Publications/p/LHCb-PAPER-2023-030.html (LHCb public pages