Quantitative Ermittlung des Fermentgehaltes und der PAS-positiven Substanzen von Knochenmarkzellen <em>in vivo</em> und <em>in vitro</em> : Saure und alkalische Phosphatase, alpha-Naphtylacetat-Esterase, Peroxydase und PAS-Reaktion in der 3-Tage-Knochenma

In smears of clot cultures of human bone marrow the content of alkaline and acid phosphatase, alpha-naphthylacetate esterase, peroxydase, and PAS-positive substances is demonstrated histochemically, The intensity of the reaction is presented as an index for the different erythroid and granulocytic precursors. In the clot cultures of a certain degree pathologically affected bone marrow the average total index of both kinds of phosphatases increases significantly from 0 to 72 hours, the average total index of the &alpha;-naphthyl-acetate esterase stays the same and the indices of peroxydase and of the PAS reaction decreases significantly already in 24 hours, but keep the same level up to the 72th hour. The PMNs matured in vitro have a bigger content of alkaline and acid phosphatase than those matured in vivo, but a smaller content of peroxydase and PAS-positive substances