43 research outputs found
In the search for the low-complexity sequences in prokaryotic and eukaryotic genomes: how to derive a coherent picture from global and local entropy measures
We investigate on a possible way to connect the presence of Low-Complexity
Sequences (LCS) in DNA genomes and the nonstationary properties of base
correlations. Under the hypothesis that these variations signal a change in the
DNA function, we use a new technique, called Non-Stationarity Entropic Index
(NSEI) method, and we prove that this technique is an efficient way to detect
functional changes with respect to a random baseline. The remarkable aspect is
that NSEI does not imply any training data or fitting parameter, the only
arbitrarity being the choice of a marker in the sequence. We make this choice
on the basis of biological information about LCS distributions in genomes. We
show that there exists a correlation between changing the amount in LCS and the
ratio of long- to short-range correlation
The rat glucocorticoid receptor integration in Nicotiana langsdorffii genome affects plant responses to abiotic stresses and to arbuscular mycorrhizal symbiosis
The present study reports evidence of the pleiotropic effects caused by the insertion of the rat glucocorticoid receptor (GR) into the genome of Nicotiana langsdorffii. Transgenic N. langsdorffii-GR plants and the wild-type genotypes were analysed for their phenotypic and physiological characteristics. The integration of the GR gene affected flowering, growth habit, leaf morphology and stomatal pattern. Furthermore, GR plants showed an increased tolerance to heavy metal, drought and heat stress as evidenced by electrolyte leakage and by cell dedifferentiation and differentiation capability after recovery from stress treatments. We also monitored the establishment of the beneficial symbiosis between transgenic plants and the mycorrhizal fungus Funneliformis mosseae whose presymbiotic growth was significantly reduced by root exudates of N. langsdorffii-GR plants. The observed pleiotropic responses of transgenic plants may be a consequence of the hormonal imbalance, putatively due to the interaction of the GR receptor with the host genetic background. Our findings suggest that N. langsdorffii-GR plants can be used as a functional model system for the study of plant responses to a series of environmental stimuli